Xiamen Amoy Diagnostics Co. | Date: 2013-11-12
Bacteriological preparations for medical and veterinary use; chemico-pharmaceutical preparations, namely, for gout, cancer; chemical preparations for pharmaceutical purposes, namely, for gout, cancer; cultures of microorganisms for medical and veterinary use; chemical preparations for medical purposes, namely, for gout, cancer; diagnostic preparations for medical purposes; chemical reagents for medical or veterinary purposes; nutritive substances for microorganisms cultures; biocides; biological preparations for medical purposes, namely, for gout, cancer.
Xiamen Amoy Diagnostics Co. | Date: 2012-02-01
Loop-shaped primer used in nucleic acid amplification and the use thereof are provided. The present invention belongs to the area of biotechnology. The primer is an oligonucleotide with 3-20 bases in both 3and 5ends which can be combined together to form a double-strand under appropriate conditions, resulting in the primer forming a stem-loop structure. The double-stranded structure is opened and the stem-loop structure dissolves when the primer recognizes and hybridizes with the target sequence. If the target sequence is not present the primer can form a stem-loop structure automatically by self-annealing. The primer can comprise a universal tag sequence or not. Together with universal tag sequence primer the primer comprising the universal tag sequence can be used for a second round of amplification. The primer of present invention has high specificity and does not form a primer dimmer. The primer of the present invention is easy to design and is suitable for measuring gene expression and detecting featues of nucleic acids such as SNPs and rare mutations.