Amoy BUCT Industrial Bio Technovation Institute

Xiamen, China

Amoy BUCT Industrial Bio Technovation Institute

Xiamen, China
SEARCH FILTERS
Time filter
Source Type

Liu H.,Beijing University of Chemical Technology | Zhao S.,Beijing University of Chemical Technology | Jin Y.,Beijing University of Chemical Technology | Yue X.,Beijing University of Chemical Technology | And 4 more authors.
Bioresource Technology | Year: 2017

Fumaric acid is an important building-block chemical. The production of fumaric acid by fermentation is possible. Loofah fiber is a natural, biodegradable, renewable polymer material with highly sophisticated and pore structure. This work investigated a new immobilization method using loofah fiber as carrier to produce fumaric acid in a stirred-tank reactor. Compared with other carriers, loofah fiber was proven to be efficiently and successfully used in the reactor. After the optimization process, 20 g addition of loofah fiber and 400 rpm agitation speed were chosen as the most suitable process conditions. 30.3 g/L fumaric acid in the broth as well as 19.16 g fumaric acid in the precipitation of solid was achieved, while the yield from glucose reached 0.211 g/g. Three batches of fermentation using the same loofah fiber carrier were conducted successfully, which meant it provided a new method to produce fumaric acid in a stirred-tank reactor. © 2017


He J.Y.,Beijing University of Chemical Technology | Wang Q.,Beijing University of Chemical Technology | Ye X.C.,Amoy BUCT Industrial Bio Technovation Institute | Wang R.Y.,Beijing University of Chemical Technology | And 3 more authors.
Material Science and Engineering - Proceedings of the 3rd Annual International Conference on Material Science and Engineering, ICMSE 2015 | Year: 2016

The influence of species and diameters of the filler on the thermal conductivities of silicone potting glue is investigated in this paper. The relationship between the species and diameters of the powder particle and the thermal conductivities of the composite material is explained on a microscopic scale. The experiment shows that the thermal conductivities of the silicone potting material are increased with the increase in the volume fraction of the filler powder. The thermal conductivities of the silicone potting material are influenced significantly by the species, diameters and different particle size complex of the thermal conductive filler when the quantity of powder filling is the same. The thermal conductivity of the potting material can be affected when the filler size is too large or too small. The complexity of the different particle size filler is conducive to improving the thermal conductivity of the potting material. © 2016 Taylor & Francis Group, London.


Liu M.,Taipei Medical University | Liu M.,Taipei Medical University Hospital | Bamodu O.A.,Taipei Medical University | Huang W.-C.,National Yang Ming University | And 13 more authors.
Toxicology and Applied Pharmacology | Year: 2017

Targeting residual self-renewing, chemoresistant cancerous cells may represent the key to overcoming therapy resistance. The entry of these quiescent cells into an activated state is associated with high metabolic demand and autophagic flux. Therefore, modulating the autophagy pathway in aggressive carcinomas may be beneficial as a therapeutic modality. In this study, we evaluated the anti-tumor activities of 4-acetylantroquinonol B (4-AAQB) in chemoresistant ovarian cancer cells, particularly its ability to modulate autophagy through autophagy-related genes (Atg). Atg-5 was overexpressed in invasive ovarian cancer cell lines and tissue (OR: 5.133; P = 0.027) and depleting Atg-5 in ES-2 cell lines significantly induced apoptosis. 4-AAQB effectively suppressed viability of various subtypes of ovarian cancer. Cells with higher cisplatin-resistance were more responsive to 4-AAQB. For the first time, we demonstrate that 4-AAQB significantly suppress Atg-5 and Atg-7 expression with decreased autophagic flux in ovarian cancer cells via inhibition of the PI3K/Akt/mTOR/p70S6K signaling pathway. Similar to Atg-5 silencing, 4-AAQB-induced autophagy inhibition significantly enhanced cell death in vitro. These results are comparable to those of hydroxychloroquine (HCQ). In addition, 4-AAQB/cisplatin synergistically induced apoptosis in ovarian cancer cells. In vivo, 4-AAQB/cisplatin also significantly induced apoptosis and autophagy in an ES-2 mouse xenografts model. This is the first report demonstrating the efficacy of 4-AAQB alone or in combination with cisplatin on the suppression of ovarian cancer via Atg-5-dependent autophagy. We believe these findings will be beneficial in the development of a novel anti-ovarian cancer therapeutic strategy. © 2017 Elsevier Inc.


Zhou Y.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Zhang X.,Beijing University of Chemical Technology | And 6 more authors.
Bioresource Technology | Year: 2014

This work investigated the capability of Rhizopus arrhizus to assimilate biodiesel-derived crude glycerol and convert it into fumaric acid. After optimizing the initial glycerol concentration, spore inoculum and yeast extract concentration, smaller pellets (0.7. mm) and higher biomass (3.11. g/L) were obtained when R. arrhizus grew on crude glycerol. It was found that crude glycerol was more suitable than glucose for smaller R. arrhizus pellet forming. When 80. g/L crude glycerol was used as carbon source, the fumaric acid production of 4.37. g/L was obtained at 192. h. With a highest concentration of 22.81. g/L achieved in the co-fermentation of crude glycerol (40. g/L) and glucose (40. g/L) at 144. h, the fumaric acid production was enhanced by 553.6%, compared to the fermentation using glycerol (80. g/L) as sole carbon source. Moreover, the production cost of fumaric acid in co-fermentation was reduced by approximately 14% compared to glucose fermentation. © 2014 Elsevier Ltd.


Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Wang F.,Beijing University of Chemical Technology | Tan T.,Beijing University of Chemical Technology | Liu L.,Beijing University of Chemical Technology
Applied Biochemistry and Biotechnology | Year: 2015

Non-edible oils are preferred raw materials for biodiesel production. However, the properties of raw materials significantly affect the synthesis process, leading to difficulties to design one process suitable for any kind of raw material. In this study, the composition of five typical non-edible oils was analyzed. The major difference was the content of free fatty acids, reflected from their acid values. The influence of different oils was investigated by using lipase from Candida sp. 99–125. At low lipase dosage and low water content, the conversion was found proportional to the acid value. However, by increasing the water content or lipase dosage, we observed that the conversions for all kinds of oils used in this study could exceed 80 %. Time course analysis indicates that the lipase used in this study catalyzed hydrolysis followed by esterification, rather than direct transesterification. Accumulation of free fatty acids at the very beginning was necessary. A high water content facilitated the hydrolysis of oils with low acid value. This lipase showed capability to transform all the oils by controlling the water content. © 2015 Springer Science+Business Media New York


Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio Technovation Institute | Wang M.,Beijing University of Chemical Technology | Zhang X.,Beijing University of Chemical Technology | And 6 more authors.
Fuel | Year: 2015

Biodiesel production from waste oil by lipase Candida sp. 99-125 assisted with cyclodextrin as additive was investigated in a solvent free system. The optimum reaction conditions was determined toward lipase dosage, agitation speed, cyclodextrin loading, water content and reuse ability of free lipase. A certain dosage of cyclodextrin showed a significant improvement of the reactions. However, the agitation speed should not be too high, to avoid breaking the weak bond between cyclodextrin and lipase. The optimal conditions were: free lipase dosage 70 U/g oil (about 0.4 wt% to the oil weight, when activity of lipase was 20,000 IU), cyclodextrin to lipase 2:1(g/g), water content 2 wt% (water/oil, g/g), and agitation speed 180 rpm. Methanol was added 30 times at 1/30 M equivalent each hour. Under the optimal conditions, the yield of FAMEs achieved 88%. After 5 cycles of reuse, no significant decrease in FAMEs yield was observed. The process has been successfully carried out at industrial scale in Shanghai, China. © 2015 Elsevier Ltd. All rights reserved.


Wang M.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Yun F.,Beijing University of Chemical Technology | And 5 more authors.
Renewable Energy | Year: 2015

Poor low temperature behavior, the critical defect of biodiesel, limits its utilization in cold districts. This research provided a new biodiesel production process by enzymatic synthesis using fusel alcohol and fatty acid esters in a solvent free system with a significant effect on the improvement of biodiesel's low temperature performance. The optimal conditions for the enzymatic reaction of fusel alcohol and waste cooking oil (WCO) esters were established: immobilized lipase 14% (w/w), total water content 5% (w/w), 40°C, three-times feeding daily, shaking speed 200rpm. The ester yield reached 90.4% under optimal conditions. The cold filter plugging point (CFPP) of the fusel alcohol esters was-11°C, 16°C lower than the methyl esters of the WCO. The green enzymatic synthesis process and the CFPP results were examined in this research. © 2015 Elsevier Ltd.


Liu H.,Beijing University of Chemical Technology | Wang W.,Beijing University of Chemical Technology | Deng L.,Beijing University of Chemical Technology | Deng L.,Amoy BUCT Industrial Bio technovation Institute | And 2 more authors.
Bioresource Technology | Year: 2015

Fumaric acid, as an important material for polymerization, is highly expected to be produced by fermentation of lignocellulosic biomass which is composed of cellulose, hemicellulose and lignin. Xylose as the main component of hemicellulose cannot be efficiently utilized by most of the common fermentation. In this study, a new strain Rhizopus arrhizus RH 7-13-9# was selected from the R. arrhizus RH 7-13 through a novel convenient and efficient selection method. Efficient production of fumaric acid (45.31. g/L) from xylose was achieved by the new strain, and the volumetric productivity was still 0.472. g/L. h. Moreover, the conversion of xylose reached 73% which is close to the theoretic yield (77%). The production of fumaric acid was increased approximate by 172%, compared with the initial strain counterpart. These results indicated that xylose, as the main component of hemicellulose, has a promising application for the production of fumaric acid on an industrial-scale. © 2015 Elsevier Ltd.


Zhang X.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Zheng Y.,Tianjin Bohai Vocational Technical College | And 4 more authors.
Catalysis Communications | Year: 2015

Sugar fatty acid esters are surfactants that are widely used in food, cosmetics, and pharmaceutical industries. In this study, enzymatic production of d-sorbitol monoesters with various fatty acids (C10-C18) was assessed. Reactions were performed in tert-butanol at 40°C, catalyzed by Candida sp. 99-125 lipase. Dissolution of the substrates was found to be the limiting step of the reaction, and hence preheating was performed to increase the solubility of d-sorbitol. Thus, the yield of d-sorbitol palmitate was increased from 7.34 to 31.6 mM. The results demonstrated that the specific lipase preferred long-chain fatty acids. The surface-active properties of the products were also determined. © 2015 Published by Elsevier B.V.


Wang M.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio Technovation Institute | Cao H.,Beijing University of Chemical Technology | And 4 more authors.
Bioresource Technology | Year: 2014

The poor low-temperature properties of biodiesel, which provokes easy crystallization at low temperature, can cause fuel line plugging and limits its blending amount with petro-diesel. This work aimed to study the production of biodiesel with a new process of improving the low temperature performance of biodiesel. Waste cooking oil was first hydrolyzed into fatty acids (FAs) by 60. g immobilized lipase and 240. g RO water in 15. h. Then, urea complexation was used to divide the FAs into saturated and unsaturated components. The conditions for complexation were: FA-to-urea ratio 1:2 (w/w), methanol to FA ratio 5:1 (v/v), duration 2. h. The saturated and unsaturated FAs were then converted to iso-propyl and methyl esters by lipase, respectively. Finally, the esters were mixed together. The CFPP of this mixture was decreased from 5. °C to -3. °C. Hydrolysis, urea complexation and enzymic catalyzed esterification processes are discussed in this paper. © 2014 Elsevier Ltd.

Loading Amoy BUCT Industrial Bio Technovation Institute collaborators
Loading Amoy BUCT Industrial Bio Technovation Institute collaborators