Amoy BUCT Industrial Bio Technovation Institute

Xiamen, China

Amoy BUCT Industrial Bio Technovation Institute

Xiamen, China

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Zhou Y.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Zhang X.,Beijing University of Chemical Technology | And 6 more authors.
Bioresource Technology | Year: 2014

This work investigated the capability of Rhizopus arrhizus to assimilate biodiesel-derived crude glycerol and convert it into fumaric acid. After optimizing the initial glycerol concentration, spore inoculum and yeast extract concentration, smaller pellets (0.7. mm) and higher biomass (3.11. g/L) were obtained when R. arrhizus grew on crude glycerol. It was found that crude glycerol was more suitable than glucose for smaller R. arrhizus pellet forming. When 80. g/L crude glycerol was used as carbon source, the fumaric acid production of 4.37. g/L was obtained at 192. h. With a highest concentration of 22.81. g/L achieved in the co-fermentation of crude glycerol (40. g/L) and glucose (40. g/L) at 144. h, the fumaric acid production was enhanced by 553.6%, compared to the fermentation using glycerol (80. g/L) as sole carbon source. Moreover, the production cost of fumaric acid in co-fermentation was reduced by approximately 14% compared to glucose fermentation. © 2014 Elsevier Ltd.


Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Wang F.,Beijing University of Chemical Technology | Tan T.,Beijing University of Chemical Technology | Liu L.,Beijing University of Chemical Technology
Applied Biochemistry and Biotechnology | Year: 2015

Non-edible oils are preferred raw materials for biodiesel production. However, the properties of raw materials significantly affect the synthesis process, leading to difficulties to design one process suitable for any kind of raw material. In this study, the composition of five typical non-edible oils was analyzed. The major difference was the content of free fatty acids, reflected from their acid values. The influence of different oils was investigated by using lipase from Candida sp. 99–125. At low lipase dosage and low water content, the conversion was found proportional to the acid value. However, by increasing the water content or lipase dosage, we observed that the conversions for all kinds of oils used in this study could exceed 80 %. Time course analysis indicates that the lipase used in this study catalyzed hydrolysis followed by esterification, rather than direct transesterification. Accumulation of free fatty acids at the very beginning was necessary. A high water content facilitated the hydrolysis of oils with low acid value. This lipase showed capability to transform all the oils by controlling the water content. © 2015 Springer Science+Business Media New York


Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio Technovation Institute | Wang M.,Beijing University of Chemical Technology | Zhang X.,Beijing University of Chemical Technology | And 6 more authors.
Fuel | Year: 2015

Biodiesel production from waste oil by lipase Candida sp. 99-125 assisted with cyclodextrin as additive was investigated in a solvent free system. The optimum reaction conditions was determined toward lipase dosage, agitation speed, cyclodextrin loading, water content and reuse ability of free lipase. A certain dosage of cyclodextrin showed a significant improvement of the reactions. However, the agitation speed should not be too high, to avoid breaking the weak bond between cyclodextrin and lipase. The optimal conditions were: free lipase dosage 70 U/g oil (about 0.4 wt% to the oil weight, when activity of lipase was 20,000 IU), cyclodextrin to lipase 2:1(g/g), water content 2 wt% (water/oil, g/g), and agitation speed 180 rpm. Methanol was added 30 times at 1/30 M equivalent each hour. Under the optimal conditions, the yield of FAMEs achieved 88%. After 5 cycles of reuse, no significant decrease in FAMEs yield was observed. The process has been successfully carried out at industrial scale in Shanghai, China. © 2015 Elsevier Ltd. All rights reserved.


Wang M.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Yun F.,Beijing University of Chemical Technology | And 5 more authors.
Renewable Energy | Year: 2015

Poor low temperature behavior, the critical defect of biodiesel, limits its utilization in cold districts. This research provided a new biodiesel production process by enzymatic synthesis using fusel alcohol and fatty acid esters in a solvent free system with a significant effect on the improvement of biodiesel's low temperature performance. The optimal conditions for the enzymatic reaction of fusel alcohol and waste cooking oil (WCO) esters were established: immobilized lipase 14% (w/w), total water content 5% (w/w), 40°C, three-times feeding daily, shaking speed 200rpm. The ester yield reached 90.4% under optimal conditions. The cold filter plugging point (CFPP) of the fusel alcohol esters was-11°C, 16°C lower than the methyl esters of the WCO. The green enzymatic synthesis process and the CFPP results were examined in this research. © 2015 Elsevier Ltd.


Liu H.,Beijing University of Chemical Technology | Wang W.,Beijing University of Chemical Technology | Deng L.,Beijing University of Chemical Technology | Deng L.,Amoy BUCT Industrial Bio technovation Institute | And 2 more authors.
Bioresource Technology | Year: 2015

Fumaric acid, as an important material for polymerization, is highly expected to be produced by fermentation of lignocellulosic biomass which is composed of cellulose, hemicellulose and lignin. Xylose as the main component of hemicellulose cannot be efficiently utilized by most of the common fermentation. In this study, a new strain Rhizopus arrhizus RH 7-13-9# was selected from the R. arrhizus RH 7-13 through a novel convenient and efficient selection method. Efficient production of fumaric acid (45.31. g/L) from xylose was achieved by the new strain, and the volumetric productivity was still 0.472. g/L. h. Moreover, the conversion of xylose reached 73% which is close to the theoretic yield (77%). The production of fumaric acid was increased approximate by 172%, compared with the initial strain counterpart. These results indicated that xylose, as the main component of hemicellulose, has a promising application for the production of fumaric acid on an industrial-scale. © 2015 Elsevier Ltd.


Chang T.-C.,Taipei Medical University | Yeh C.-T.,Taipei Medical University | Adebayo B.O.,Taipei Medical University | Lin Y.-C.,Taipei Medical University | And 10 more authors.
Toxicology and Applied Pharmacology | Year: 2015

4-Acetylantroquinonol B (4-AAQB), closely related to the better known antroquinonol, is a bioactive isolate of the mycelia of Antrodia camphorata, a Taiwanese mushroom with documented anti-inflammatory, hypoglycemic, vasorelaxative, and recently demonstrated, antiproliferative activity. Based on its traditional use, we hypothesized that 4-AAQB may play an active role in the suppression of cellular transformation, tumor aggression and progression, as well as chemoresistance in colorectal carcinoma (CRC). In this study, we investigated the antiproliferative role of 4-AAQB and its underlying molecular mechanism. We also compared its anticancer therapeutic potential with that of antroquinonol and the CRC combination chemotherapy of choice - folinic acid, fluorouracil and oxaliplatin (FOLFOX). Our results showed that 4-AAQB was most effective in inhibiting tumor proliferation, suppressing tumor growth and attenuating stemness-related chemoresistance. 4-AAQB negatively regulates vital oncogenic and stem cell maintenance signal transduction pathways, including the Lgr5/Wnt/β-catenin, JAK-STAT, and non-transmembrane receptor tyrosine kinase signaling pathways, as well as inducing a dose-dependent downregulation of ALDH and other stemness related factors. These results were validated in vivo, with animal studies showing 4-AAQB possessed comparable tumor-shrinking ability as FOLFOX and potentiates ability of the later to reduce tumor size. Thus, 4-AAQB, a novel small molecule, projects as a potent therapeutic agent for monotherapy or as a component of standard combination chemotherapy. © 2015 Elsevier Inc.


Zhang X.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio technovation Institute | Zheng Y.,Tianjin Bohai Vocational Technical College | And 4 more authors.
Catalysis Communications | Year: 2015

Sugar fatty acid esters are surfactants that are widely used in food, cosmetics, and pharmaceutical industries. In this study, enzymatic production of d-sorbitol monoesters with various fatty acids (C10-C18) was assessed. Reactions were performed in tert-butanol at 40°C, catalyzed by Candida sp. 99-125 lipase. Dissolution of the substrates was found to be the limiting step of the reaction, and hence preheating was performed to increase the solubility of d-sorbitol. Thus, the yield of d-sorbitol palmitate was increased from 7.34 to 31.6 mM. The results demonstrated that the specific lipase preferred long-chain fatty acids. The surface-active properties of the products were also determined. © 2015 Published by Elsevier B.V.


Wang M.,Beijing University of Chemical Technology | Nie K.,Beijing University of Chemical Technology | Nie K.,Amoy BUCT Industrial Bio Technovation Institute | Cao H.,Beijing University of Chemical Technology | And 4 more authors.
Bioresource Technology | Year: 2014

The poor low-temperature properties of biodiesel, which provokes easy crystallization at low temperature, can cause fuel line plugging and limits its blending amount with petro-diesel. This work aimed to study the production of biodiesel with a new process of improving the low temperature performance of biodiesel. Waste cooking oil was first hydrolyzed into fatty acids (FAs) by 60. g immobilized lipase and 240. g RO water in 15. h. Then, urea complexation was used to divide the FAs into saturated and unsaturated components. The conditions for complexation were: FA-to-urea ratio 1:2 (w/w), methanol to FA ratio 5:1 (v/v), duration 2. h. The saturated and unsaturated FAs were then converted to iso-propyl and methyl esters by lipase, respectively. Finally, the esters were mixed together. The CFPP of this mixture was decreased from 5. °C to -3. °C. Hydrolysis, urea complexation and enzymic catalyzed esterification processes are discussed in this paper. © 2014 Elsevier Ltd.


Zhao K.,Beijing University of Chemical Technology | Zhao K.,China National Publications Import and Export Group Corporation | Di Q.,Beijing University of Chemical Technology | Cao X.,Beijing University of Chemical Technology | And 4 more authors.
Sustainability (Switzerland) | Year: 2016

Lipase from Candida sp. 99-125 is widely employed to catalyzed transesterification and can be used for biodiesel production. In this study, the lipase was immobilized by combined adsorption and entrapment to catalyze biodiesel production from waste cooking oil (WCO) via transesterification, and investigating co-immobilizing agents as additives according to the enzyme activity. The addition of the mixed co-immobilizing agents has positive effects on the activities of the immobilized lipase. Three different immobilizing methods were compared by the conversion ratio of biodiesel and structured by Atom Force Microscopy (AFM) and Scanning Electron Microscopy (SEM), respectively. It was found that entrapment followed by adsorption was the best method. The effect of the co-immobilizing agent amount, lipase dosage, water content, and reuse ability of the immobilized lipase was investigated. By comparison with previous research, this immobilized lipase showed good reuse ability: the conversion ratio excesses 70% after 10 subsequent reactions, in particular, was better than Novozym435 and TLIM on waste cooking oil for one unit of lipase. © 2016 by the authors.

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