Azizi H.,Amol University of Special Modern Technologies |
Skutella T.,University of Heidelberg |
Shahverdi A.,Royan Institute for Stem Cell Biology and Technology
Cell Journal | Year: 2017
Objective: The properties of self-renewal and division in spermatogonial stem cells (SSCs) support spermatogenesis. There is a number of reported methods for in vitro SSC culture systems. The development of a culture system that effectively supports isolation and self-renewal of germline stem cells (GSCs) is of tremendous benefit for clinical trials, experimental research, and as potential treatment for male infertility. The current study aims to consider the cultivation and behavior of GSCs in a non-adherent culture system. Materials and Methods: In this experimental study, we cultured testicular cells from neonatal mice in agarose coated plates in the presence of Dulbecco's modified Eagle's medium (DMEM) medium (CTRL group), 10% fetal bovine serum (FBS)+DMEM (10% group), and growth factor (G group) that contained 2% FBS, glial cell-derived neurotrophic factor (GDNF), epidermal growth factor (EGF), and fibroblast growth factor (FGF). Mouse spermatogonial stem-like colonies were isolated approximately 3 weeks after digestion of the testis tissue. After passages 2-3, the identity of the mouse spermatogonial stem-like cells was confirmed by immunocytochemistry, reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry against the germ cell markers α6, β1, c-Kit, Thy-1, c-Ret, Plzf, and Oct4. The statistical significance between mean values in different groups was determined by one-way analysis of variance (ANOVA). Results: We observed spermatogonial stem-like colonies in the G and 10% groups, but not the CTRL group. Immunocytochemistry, flow cytometry, and RT-PCR confirmed expressions of germ cell markers in these cells. In the spermatogonial stem-like cells, we observed a significant expression (P<0.05) of germ cell markers in the G and 10% groups versus the testis cells (T). Their proliferative and apoptotic activities were examined by Ki67 and PI/annexin V-FITC. Alkaline phosphatase assay showed that mouse spermatogonial stem-like colonies were partially positive. Conclusion: A non-adherent culture system could provide a favorable method for in vitro short-term culture of spermatogonial stem-like cell colonies.
Nikpay A.,Amol University of Special Modern Technologies
Journal of Arthropod-Borne Diseases | Year: 2016
Background: Rhipicephalus (Boophilus) annulatus tick is one of the most important ectoparasite of cattle. Recently, several laboratories in the world have been concentrated on immunizing cattle against tick using various types of tissue extracts of ticks. The aim of this study was to evaluate the effect of immunization of cattle with tick salivary gland extract on biological parameters of ticks and humoral immune responses of cattle. Methods: Fourteen more dominant protein bands identified as immunogenic by Western-blot analysis were eluted from polyacrylamide gel. Test and control groups were injected three times with eluted proteins and sterile PBS (pH= 7.2) respectively with equivalent amount of adjuvant. After four weeks a tick challenge was performed. Finally, biological parameters of collected engorged female ticks were recorded and humoral immune responses to immunization measured by ELISA. Results: The results indicated immunization of cattle resulted in reduction in mean tick counts, attachment, engorgement weights, feeding index, egg mass weight, hatchability and fertility index (respectively 63.1%, 62.6%, 30.2%, 36.4%, 40%, 78.7% and 13.3%) and increased duration of feeding, pre-oviposition and incubation period of eggs (respectively 8.6%, 45 and 31.34%). All changes were statistically significant (P < 0.05). Results showed an increase in antibody production of test group from the first week after immunization. The antibody level was boosted following tick infestation. Conclusion: This investigation indicates that immunization of cattle with these antigens could induce a protective immune response against Rh. (B.) annulatus tick that would be expected to provide a safe non-chemical means of tick control.
Nazari F.,Amol University of Special Modern Technologies |
Moravvej-Farshi M.K.,Tarbiat Modares University
IEEE Journal of Quantum Electronics | Year: 2016
We report the design procedure for a broadband multi-channel cavity-less optical isolator composed of a triangular perturbed nonlinear parity time (PT) symmetric lattice. The interplay between the nonlinearity and the PT-symmetric lattice that results in nonreciprocal transmission enables us to design the isolator outputs and inputs positions as desired. In the transmitting regime, a number of solitons that are simultaneously launched into individual inputs positioned appropriately, on the waveguides' gain sides in the periodic lattice, swing along the waveguide until they are self-trapped along the respective waveguides axes, where the isolator outputs are located. In the isolating regime, however, the solitons that are launched into the inputs - i.e., the outputs of the transmitting regime - propagate along the waveguides axes, until they exit from the new outputs. Simulations show that the neighboring soliton trajectories in each regime are completely isolated and so are the forward and reverse trajectories in each waveguide cell. Moreover, operation of this cavity-less PT-symmetric isolator that is designed on an inhomogeneous slab waveguide does not suffer from the narrow frequency band limitation, unlike their cavity-based counterparts. © 1965-2012 IEEE.
Mohammadi E.,University of Tehran |
Nassiri S.M.,University of Tehran |
Rahbarghazi R.,Tabriz University of Medical Sciences |
Siavashi V.,Guilan University |
Araghi A.,Amol University of Special Modern Technologies
Cell and Tissue Research | Year: 2015
Efficacy of therapeutic angiogenesis needs a comprehensive understanding of endothelial cell (EC) function and biological factors and cells that interplay with ECs. Stem cells are considered the key components of pro- and anti-angiogenic milieu in a wide variety of physiopathological states, and interactions of EC-stem cells have been the subject of controversy in recent years. In this study, the potential effects of three tissue-specific adult stem cells, namely rat marrow-derived mesenchymal stem cells (rBMSCs), rat adipose-derived stem cells (rADSCs) and rat muscle-derived satellite cells (rSCs), on the endothelial activation of key angiogenic signaling molecules, including VEGF, Ang-2, VEGFR-2, Tie-2, and Tie2-pho, were investigated. Human umbilical vein endothelial cells (HUVECs) and rat lung microvascular endothelial cells (RLMECs) were cocultured with the stem cells or incubated with the stem cell-derived conditioned media on Matrigel. Following HUVEC-stem cell coculture, CD31-positive ECs were flow sorted and subjected to western blotting to analyze potential changes in the expression of the pro-angiogenic signaling molecules. Elongation and co-alignment of the stem cells were seen along the EC tubes in the EC-stem cell cocultures on Matrigel, with cell-to-cell dye communication in the EC-rBMSC cocultures. Moreover, rBMSCs and rADSCs significantly improved endothelial tubulogenesis in both juxtacrine and paracrine manners. These two latter stem cells dynamically up-regulated VEGF, Ang-2, VREGR-2, and Tie-2 but down-regulated Tie2-pho and the Tie2-pho/Tie-2 ratio in HUVECs. Induction of pro-angiogenic signaling in ECs by marrow- and adipose-derived MSCs further indicates the significance of stem cell milieu in angiogenesis dynamics. © 2015, Springer-Verlag Berlin Heidelberg.
Nabavi S.F.,Baqiyatallah Medical Sciences University |
Daglia M.,University of Pavia |
Moghaddam A.H.,Amol University of Special Modern Technologies |
Moghaddam A.H.,University of Mazandaran |
And 2 more authors.
Comprehensive Reviews in Food Science and Food Safety | Year: 2014
Curcumin, the natural yellow-colored active principle, also called turmeric yellow, extracted from the perennial herb Curcuma longa L., has potent biological and pharmacological properties such as antioxidant, anti-inflammatory, antifungal, antibacterial, anti-ischemic, antitumor, and anticancer actions. The molecular mechanism of the hepatoprotective action of curcumin is due to its antioxidant properties and inhibitory activity against nuclear factor (NF)-κB that regulates different proinflammatory and profibrotic cytokines. Overall, scientific reports demonstrate that curcumin has high therapeutic ability for treating hepatic disorders. Here is a systematic discussion of the hepatoprotective activity of curcumin and its possible mechanisms of actions. © 2013 Institute of Food Technologists®.
Azizkhani M.,Amol University of Special Modern Technologies |
Tooryan F.,Amol University of Special Modern Technologies
Journal of Mazandaran University of Medical Sciences | Year: 2016
Background and purpose: Since long time ago herbal products and spices have been used as powders, essential oils (EOs), and extracts. They consist of antimicrobial components and could be applied as natural food preservatives. In the present work, the antiviral effect of cinnamon, rosemary, and zataria EOs has been investigated against norovirus surrogates including feline calicivirus (FCV) and murine norovirus (MNV). Materials and methods: Different concentrations of EOs were individually mixed with each virus at titers of ca. 7-8 log TCID50/ml and incubated for 2 h at 4ºC and 37 ºC. The infectivity of the recovered viruses was evaluated by cell-culture assays. Results: At 37 ºC, 3% of cinnamon, 2.5% of rosemary and 0.1% of zataria EOs decreased the FCV titers by 2. 38, 3.38, and 4.51 log TCID50/ml, respectively. At lower concentrations they were found to be less effective. Also, different concentrations of EOs decreased MNV titers by 0.25-1.44 log TCID50/ml. The maximum titer reduction (4.51 log TCID50/ml) was achieved when the FCV was treated at 37 ºC with 0.1% of zataria EO. Conclusion: The reduction on the infectivity titers found for the tested norovirus surrogates with these EOs highlighted their potential role as biopreservatives to improve food hygiene and shelflife. © 2016, Mazandaran University of Medical Sciences. All rights reserved.
Mahmoudi A.H.,Babol Noshirvani University of Technology |
Ramiar A.,Babol Noshirvani University of Technology |
Esmaili Q.,Amol University of Special Modern Technologies
Energy Conversion and Management | Year: 2016
In this paper, effects of inhomogeneous compression of GDL at the cathode side of proton-exchange membrane (PEM) fuel cell with interdigitated flow field on water management and cell performance has been numerically investigated.A 2D, isothermal, two-phase and multi-component transport model has been used in order to simulate the transport phenomena. The model consists the gas diffusion layer and an ultra- thin layer as catalyst. The model results were in good agreement with the published data found in the literature. The results of this study prove the effects of considering GDL deformation on decreasing water removal from cell and also on decreasing reactants diffusion transports to the reaction sites, and thus dropping the cell performance as expressed by a drop in the cell limiting current density and maximum output power. Also, it has been proven that the compression level of 35% has no significant effects on the performance of the cell in single-phase regions, but effects of this level of compression are very considerable on the performance of the cell in the two-phase region. As a result, limiting current density and maximum out power of the cell were dropped by approximately 25%. Moreover, increasing the compression level from 10% to 35% led to an increase in the flooding of GDL and reactant transport was limited and performance loss increased (even in single-phase regions). At high compression level of 35%, the limiting current density and maximum output power had a very considerable drop of 25.1%, which indicates the importance of considering the effects of high clamping pressures in PEMFC modelling. © 2015 Elsevier Ltd. All rights reserved.
Rostami-Dogolsara B.,Tarbiat Modares University |
Moravvej-Farshi M.K.,Tarbiat Modares University |
Nazari F.,Amol University of Special Modern Technologies
Physical Review B - Condensed Matter and Materials Physics | Year: 2016
We report the design procedure for an acoustic add-drop filter (ADF) composed of two line-defect waveguides coupled through a ring resonator cavity (RRC) all based on a phononic crystal (PnC) platform. Using finite difference time domain and plane wave expansion methods, we study the propagation of acoustic waves through the PnC based ADF structures. Numerical results show that the quality factor for the ADF with a quasisquare ring resonator with a frequency band of 95 Hz centered about 75.21 kHz is Q ∼ 800. We show that the addition of an appropriate scatterer at each RRC corner can reduce the scattering loss, enhancing the quality factor and the transmission efficiency. Moreover, it is also shown that by increasing the coupling gaps between the RRC and waveguides the quality factor can be increased by ∼25 times, at the expense of a significant reduction in the transmission efficiency this is attributed to the enhanced selectivity in expense of weakened coupling. Finally, by varying the effective path length of the acoustic wave in the RRC, via selectively varying the inclusions physical and geometrical properties, we show how one can ultra-fine and fine-tune the resonant frequency of the ADF. © 2016 American Physical Society. ©2016 American Physical Society.
Ramiar A.,Babol Noshirvani University of Technology |
Mahmoudi A.H.,Babol Noshirvani University of Technology |
Esmaili Q.,Amol University of Special Modern Technologies |
Abdollahzadeh M.,University of Porto |
Abdollahzadeh M.,University of Beira Interior
Energy | Year: 2016
In this paper, a numerical study is conducted in order to investigate the effect of pulsation of air flow at the cathode side of Proton Exchange Membrane (PEM) fuel cell with interdigitated flow field. A two dimensional, isothermal, two-phase, unsteady multi-component transport model is used in order to simulate the transport phenomena. The obtained results are discussed in terms of the influence of flow pulsation on water management and cell performance. The results prove the effectiveness of flow pulsation on improving water removal from cell, enhancing reactants transports to the reaction sites, and increasing the cell performance expressed by increment in the cell limiting current density and maximum output power. The effects of pulsation frequency (f), amplitude (Amp), and mean inlet pressure (Pin) on the performance and the output power of the cell, are also investigated. The performance of the cell has no dependency on the frequency range considered in this study. However, as the pulsation amplitude increases the increment in the cell performance is more obvious. Moreover, applying flow pulsation at low flow rates leads to higher efficiency in water removal and performance enhancement. © 2015 Elsevier Ltd.
Rakei A.,University of Tehran |
Maali-Amiri R.,University of Tehran |
Zeinali H.,University of Tehran |
Ranjbar M.,Amol University of Special Modern Technologies
Protoplasma | Year: 2016
Cold stress (CS) signals are translated into physiological changes as products of direct and/or indirect of gene expression regulated by different factors like DNA methylation. In this study, some of these factors were comparatively studied in two chickpea (Cicer arietinum L.) genotypes (Sel96Th11439, cold-tolerant genotype, and ILC533, cold susceptible one) under control (23 °C) and days 1, 3, and 6 after exposing the seedlings to CS (4 °C). Under CS, tolerant genotype prevented H2O2 accumulation which led to a decrease in damage indices (malondialdehyde and electrolyte leakage index) compared to susceptible one. The significant activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, and polyphenol oxidase) along with a significant proportion of change in DNA methylation/demethylation patterns were often effective factors in preserving cell against cold-induced oxidative stress. Chickpea cells in response to CS changed access to their genome as the number of bands without change from day 1 to day 6 of exposure to CS particularly in tolerant genotype was decreased. During CS, the methylation level was higher compared to demethylation (29.05 vs 19.79 %) in tolerant genotype and (27.92 vs 22.09 %) in susceptible one. However, for prolonged periods of CS, changes in demethylated bands in tolerant genotype were higher than that of in susceptible one (9.24 vs 4.13 %), indicating higher potential for activation of CS responsive genes. Such a status along with higher activity of antioxidants and less damage indices could be related to cold tolerance (CT) mechanisms in chickpea. Sequencing analysis confirmed the important role of some specific DNA sequences in creating CT with possible responsive components involved in CS. Thus, dynamic assessment using multi-dimensional approaches allows us to progressively fill in the gaps between physio-biochemical and molecular events in creating CT, to comprehend better the nature of the plant stress response and molecular mechanisms behind. © 2015, Springer-Verlag Wien.