Amity Institute of Biotechnology

Greater Noida, India

Amity Institute of Biotechnology

Greater Noida, India
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Gautam S.,Indian Institute of Technology Delhi | Debnath N.,Amity Institute of Biotechnology | Ghosh D.,Amity University
Nanoscience and Nanotechnology - Asia | Year: 2016

Eco-friendly synthesis of nanoparticles (NP) using plant extract has become very popular among nanoscience researchers. Here, we report synthesis of silver nanoparticles (AgNPs) using leaf extracts of Helianthus annuus and Mentha longifolia. These NPs were characterized by UV-Vis, TEM, XRD, Fluorescence and FTIR spectroscopy and were found to have roughly spherical morphology with average particle size of 10-25 nm in diameter. Thermal stability of the nanomaterial was estimated using TGA/DSC analysis. Antimicrobial activity of these AgNPs was also screened against Escherichia coli and the antibacterial effect was also monitored using SEM. The AgNPs were found to be better antimicrobial agent compared to standard antibiotics like ampicillin or kanamycin. © 2016 Bentham Science Publishers.

Shukla S.,Institute of Cytology and Preventive Oncology ICMR | Mahata S.,Institute of Cytology and Preventive Oncology ICMR | Shishodia G.,Institute of Cytology and Preventive Oncology ICMR | Pande S.,Institute of Cytology and Preventive Oncology ICMR | And 8 more authors.
Indian Journal of Medical Research | Year: 2014

Background & objectives: High-risk human papilloma virus (HR-HPV) infection and its integration in host genome is a key event in malignant transformation of cervical cells. HPV16 being a dominant HR-HPV type, we undertook this study to analyze if viral load and physical state of the virus correlated with each other in the absence of other confounding variables and examined their potential as predictors of progressive cervical lesions. Methods: Both, viral load and integration status of HPV16 were determined by real time URR PCR and estimation of E2:E6 ratio in a total of 130 PGMY-RLB -confirmed, monotypic HPV16-infected cervical DNA samples from biopsies of cytology-confirmed low grade (LSIL, 30) and high grade (HSIL, 30), and invasive carcinoma, (squamous cell carcinoma SCC, 70) cases. Results: Investigation of DNA samples revealed a gradual increase in HPV16 viral load over several magnitudes and increased frequency of integration from LSIL to HSIL and HSIL to invasive cancer in relation to the severity of lesions in monotypic HPV16-infected cervical tissues. In a substantial number of precancer (11/60) and cancer cases (29/70), HPV16 was detected in concomitant mixed form. The concomitant form of HPV16 genome carried significantly higher viral load. Interpretation & conclusions: Overall, viral load and integration increased with disease severity and could be useful biomarkers in disease progression, at least, in HPV16-infected cervical pre-cancer and cancer lesions.

Banerjee M.,Indian Institute of Technology Guwahati | Siddique S.,Health Canada | Mukherjee S.,AMITY Institute of Biotechnology | Roychoudhury S.,Central Pollution Control Board | And 3 more authors.
International Journal of Hygiene and Environmental Health | Year: 2012

Exposure to poor air quality is associated with a multitude of hematological and immunological alterations. Cardio vascular diseases, rather than respiratory ailments, are the most important cause of death from air pollution exposure. Thus, hematological, immunological and cardiovascular alterations in healthy individuals exposed to vehicular pollution (one of the leading source of air pollution in growing metropolitan cities) are investigated in this study. A total number of 2218 (21-65 years old) adults residing in Delhi participated in this study. As control, 642 age and sex matched healthy subjects from the rural areas of Uttaranchal were enrolled. Arterial blood pressure (BP) was measured by a sphygmomanometer. Blood samples were collected and routine hematology was done. Lymphocyte subset analysis and platelet P-selectin expression was measured by flow cytometry. Air quality data was collected from Central and State Pollution Control Boards and was also measured onsite by portable, battery-operated laser photometer. The prevalence of hypertension was nearly 4-times higher in Delhi when compared to the control. Platelet P-selectin was remarkably upregulated in residents of Delhi. They had depleted number of CD4+ T-helper cells and CD19+ B cells but elevated level of CD56+ natural killer cells. Altered lymphocyte subtypes and increased number of P-selectin-positive platelets suggest altered immunity (that may compromise body's defense against infections) and hypercoagulable state, a risk factor for cardiovascular diseases. The current study has identified poor air quality of Delhi as a key contributor to several adverse health conditions experienced by the general population of the city, which not only makes the quality of life compromised but also put them at a greater risk of developing cardiovascular ailments later in life. © 2011 Elsevier GmbH.

Chalivendra S.C.,Colorado State University | Chalivendra S.C.,Valent BioSciences Corporation | Lopez-Casado G.,Cornell University | Lopez-Casado G.,University of Malaga | And 12 more authors.
Journal of Experimental Botany | Year: 2013

Although self-incompatibility (SI) in plants has been studied extensively, far less is known about interspecifc reproductive barriers. One interspecifc barrier, known as unilateral incongruity or incompatibility (UI), occurs when species display unidirectional compatibility in interspecifc crosses. In the wild tomato species Solanum pennellii, both SI and self-compatible (SC) populations express UI when crossed with domesticated tomato, offering a useful model system to dissect the molecular mechanisms involved in reproductive barriers. In this study, the timing of reproductive barrier establishment during pistil development was determined in SI and SC accessions of S. pennellii using a semi-in vivo system to track pollen-tube growth in developing styles. Both SI and UI barriers were absent in styles 5 days prior to flower opening, but were established by 2 days before flower opening, with partial barriers detected during a transition period 3-4 days before flower opening. The developmental expression dynamics of known SI factors, S-RNases and HT proteins, was also examined. The accumulation of HT-A protein coincided temporally and spatially with UI barriers in developing pistils. Proteomic analysis of stigma/styles from key developmental stages showed a switch in protein profiles from cell-division-associated proteins in immature stigma/styles to a set of proteins in mature stigma/ styles that included S-RNases, HT-A protein and proteins associated with cell-wall loosening and defense responses, which could be involved in pollen-pistil interactions. Other prominent proteins in mature stigma/styles were those involved in lipid metabolism, consistent with the accumulation of lipid-rich material during pistil maturation. © 2012 The Author. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

PubMed | Brandeis University, Stephens College, Amity Institute of Biotechnology and Amity Institute of Nanotechnology
Type: | Journal: Journal of colloid and interface science | Year: 2016

Alginate microparticles and nanoparticles crosslinked with Ca

Sun Q.,University of Delaware | Madan B.,Amity Institute of Biotechnology | Tsai S.-L.,National Taiwan University of Science and Technology | Delisa M.P.,Cornell University | Chen W.,University of Delaware
Chemical Communications | Year: 2014

For the first time, artificial cellulosome structures were created on DNA scaffolds based on zinc finger protein (ZFP)-guided assembly. These resulting two-component cellulosome structures exhibited enhancement in cellulose hydrolysis compared to the non-complexed mixture depending on the number of CBMs and cellulases assembled. This journal is © The Royal Society of Chemistry.

Trilok-Kumar G.,University of Delhi | Arora H.,Amity Institute of Biotechnology | Rajput M.,University of Delhi | Chellani H.,Safdarjung Hospital | And 7 more authors.
European Journal of Clinical Nutrition | Year: 2012

BACKGROUND/OBJECTIVES: Vitamin D deficiency has been associated with impaired resistance to infection, which may be mediated by alterations in cytokine responses. We investigated the effect of vitamin D supplementation to infants on whole blood in-vitro cytokine production and on the inflammatory marker, plasma C-reactive protein (CRP). SUBJECTS/METHODS: Blood samples were taken at 6 months of age from infants participating in the DIVIDS (Delhi Infant Vitamin D Supplementation) randomized controlled trial of weekly vitamin D supplements (1400IU=recommended intake) from birth to 6 months with the aim of decreasing mortality and severe morbidity. We measured plasma CRP and whole blood in-vitro production of tumour necrosis factor-α (TNFα), interferon-γ (INFγ), interleukin (IL)-10 and IL-13 following no stimulation or stimulation with lipopolysaccharide or phytohemagglutinin. RESULTS: Although the intervention improved vitamin D status in a severely deficient population, there were no differences between treatment groups in plasma CRP or in the production of any of the cytokines in either unstimulated or stimulated cultures. Recent illness had limited association with immunological markers. Plasma 25-hydroxyvitamin D levels were not associated with CRP or production of any cytokines. CONCLUSIONS: Vitamin D supplementation did not affect plasma CRP or whole blood cytokine production of vitamin D-deficient low birth weight infants. This is consistent with the lack of effect of vitamin D on mortality and severe morbidity among infants in the DIVIDS trial. © 2012 Macmillan Publishers Limited All rights reserved.

Kumar V.,Jaypee University of Information Technology | Shitiz K.,Jaypee University of Information Technology | Chauhan R.S.,Jaypee University of Information Technology | Sood H.,Jaypee University of Information Technology | Tandon C.,Amity Institute of Biotechnology
Journal of Plant Biochemistry and Biotechnology | Year: 2016

Picrosides, the terpenoids synthesized by Picrorhiza kurroa, have ample usage in medicine. Identification of the regulatory enzymes involved in picroside biosynthesis needs to be explored for improving the level of these secondary metabolites. Current efforts are based on the analysis of secondary metabolism in picroside biosynthesis but its interpretation is limited by the lack of information on the involvement of primary metabolic pathways. The present study investigated the connection of primary metabolic enzymes with the picrosides levels in P. kurroa. The results showed changes in the catalytic activities as well as in the gene expression profiles of hexokinase, pyruvate kinase, isocitrate dehydrogenase, malate dehydrogenase, and NADP+-malic enzyme in congruence with picroside-I content under different conditions of P. kurroa growth, which indicates the role of these enzymes in the accumulation of picrosides. The significant correlation coefficients (p < 0.05) observed between gene expression and enzyme activity underline the role of integrative studies for a better understanding of connecting links between metabolic pathways leading to picroside biosynthesis. This is apparently the first report on the involvement of glycolytic and TCA cycle enzymes in the accumulation of picrosides in P. kurroa. © 2015, Society for Plant Biochemistry and Biotechnology.

Kumar V.,Jaypee University of Information Technology | Sharma N.,Jaypee University of Information Technology | Shitiz K.,Jaypee University of Information Technology | Singh T.R.,Jaypee University of Information Technology | And 3 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2015

Picroside-I (P-I) is a member of the iridoid glycosides family of natural products, which are used to treat liver disorders. The growing medicinal need for this benign compound has stimulated the present study to identify genes important for the biosynthesis of P-I. In this study, molecular screens have been generated using gene expression patterns obtained by quantitative RT-PCR which have extended the knowledge of genes associated with P-I biosynthesis. A total of 13 genes encoding the rate limiting enzymes of different pathways, were analyzed by qRT-PCR in Picrorhiza kurroa shoots collected at 0, 10, 20, 30 and 40 days. The results showed that five of the genes (HK, DXPS, ISPD, HMGR and PMK) are supposed to be essential for P-I biosynthesis up to 20 days while DAHPS and G-10-H, in conjunction might assist P-I biosynthesis between 20 and 30 days of P. kurroa growth. This is apparently the first report on the molecular aspects of different pathways integrated in P-I biosynthesis. Moreover, principal component analysis prediction also corroborated the genes selection by identifying genes signatures for different samples (collected at different time intervals) and supported the link between samples and gene expression patterns. Overall, this study capitalizes on dynamic gene expression patterns obtained in response to the P-I stimuli under different stages of P. kurroa growth which are likely to define the regulatory steps in P-I biosynthesis. © 2015 Springer Science+Business Media Dordrecht

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