American Cancer Society, American Society for Colposcopy and Cervical Pathology, and American Society for Clinical Pathology screening guidelines for the prevention and early detection of cervical cancer
Saslow D.,Breast and Gynecologic Cancer |
Solomon D.,U.S. National Institutes of Health |
Lawson H.W.,Emory University |
Killackey M.,Sloan Kettering Cancer Center |
And 15 more authors.
CA Cancer Journal for Clinicians | Year: 2012
An update to the American Cancer Society (ACS) guideline regarding screening for the early detection of cervical precancerous lesions and cancer is presented. The guidelines are based on a systematic evidence review, contributions from 6 working groups, and a recent symposium cosponsored by the ACS, the American Society for Colposcopy and Cervical Pathology, and the American Society for Clinical Pathology, which was attended by 25 organizations. The new screening recommendations address age-appropriate screening strategies, including the use of cytology and high-risk human papillomavirus (HPV) testing, follow-up (eg, the management of screen positives and screening intervals for screen negatives) of women after screening, the age at which to exit screening, future considerations regarding HPV testing alone as a primary screening approach, and screening strategies for women vaccinated against HPV16 and HPV18 infections. CA Cancer J Clin 2012. © 2012 American Cancer Society. Copyright © 2012 American Cancer Society, Inc.
Jiang P.,Monell Chemical Senses Center |
Josue J.,Monell Chemical Senses Center |
Li X.,Monell Chemical Senses Center |
Li X.,AmeriPath |
And 7 more authors.
Proceedings of the National Academy of Sciences of the United States of America | Year: 2012
Mammalian sweet taste is primarily mediated by the type 1 taste receptor Tas1r2/Tas1r3, whereas Tas1r1/Tas1r3 act as the principal umami taste receptor. Bitter taste is mediated by a different group of G protein-coupled receptors, the Tas2rs, numbering 3 to ∼66, depending on the species. We showed previously that the behavioral indifference of cats toward sweet-tasting compounds can be explained by the pseudogenization of the Tas1r2 gene, which encodes the Tas1r2 receptor. To examine the generality of this finding, we sequenced the entire coding region of Tas1r2 from 12 species in the order Carnivora. Seven of these nonfeline species, all of which are exclusive meat eaters, also have independently pseudogenized Tas1r2 caused by ORF-disrupting mutations. Fittingly, the purifying selection pressure is markedly relaxed in these species with a pseudogenized Tas1r2. In behavioral tests, the Asian otter (defective Tas1r2) showed no preference for sweet compounds, but the spectacled bear (intact Tas1r2) did. In addition to the inactivation of Tas1r2, we found that sea lion Tas1r1 and Tas1r3 are also pseudogenized, consistent with their unique feeding behavior,which entails swallowing food whole without chewing. The extensive loss of Tas1r receptor function is not restricted to the sea lion: the bottlenose dolphin, which evolved independently from the sea lion but displays similar feeding behavior, also has all three Tas1rs inactivated, and may also lack functional bitter receptors. These data provide strong support for the view that loss of taste receptor function in mammals is widespread and directly related to feeding specializations.
Han B.,Center Hospitalier Of Luniversite Of Montreal Crchum |
Luo H.,Center Hospitalier Of Luniversite Of Montreal Crchum |
Raelson J.,PGX Services |
Huang J.,University of North Carolina at Chapel Hill |
And 5 more authors.
Human Molecular Genetics | Year: 2014
Transforming growth factor beta-induced (TGFBI/βIG-H3), also known as βig-H3, is a protein inducible by TGFβ1 and secreted by many cell types. It binds to collagen, forms part of the extracellular matrix and interacts with integrins on the cell surface. Recombinant TGFBI and transgenic TGFBI overexpression can promote both islet survival and function. In this study, we generated TGFBI KO mice and further assessed TGFBI function and signaling pathways in islets. Islets from KO mice were of normal size and quantity, and these animals were normoglycemic. However, KO islet survival and function was compromised in vitro. In vivo, KO donor islets became inferior to wild-type donor islets in achieving normoglycemia when transplanted into KO diabetic recipients. TGFBI KO mice were more prone to straptozotocin-induced diabetes than the wild-type counterpart. Phosphoprotein array analysis established that AKT1S1, a molecule linking the AKT and mTORC1 signaling pathways, wasmodulated by TGFBI in islets.Phosphorylation of four molecules in the AKT and mTORC1 signaling pathway, i.e. AKT, AKT1S1, RPS6 and EIF4EBP1, was upregulated in islets upon TGFBI stimulation. Suppression of AKT activity by a chemical inhibitor, or knockdown of AKT1S1, RPS6 and EIF4EBP1 expression by small interfering RNA, modulated is let survival, proving the relevance of these molecules in TGFBI-triggered signaling. Human genetic studies revealed that in the TGFBI gene and its vicinity, three single-nucleotide polymorphismswere significantly associated with type 1diabetes risks,andonewith type 2 diabetes risks.Ourstudy suggests that TGFBI is a potential risk gene for human diabetes. © The Author 2014. Published by Oxford University Press. All rights reserved.
Clark K.L.,University of North Florida |
Leydet B.F.,Louisiana State University |
Journal of Medical Microbiology | Year: 2014
The present study investigated the cause of illness in human patients primarily in the southern USA with suspected Lyme disease based on erythema migrans-like skin lesions and/or symptoms consistent with early localized or late disseminated Lyme borreliosis. The study also included some patients from other states throughout the USA. Several PCR assays specific for either members of the genus Borrelia or only for Lyme group Borrelia spp. (Borrelia burgdorferi sensu lato), and DNA sequence analysis, were used to identify Borrelia spp. DNA in blood and skin biopsy samples from human patients. B. burgdorferi sensu lato DNA was found in both blood and skin biopsy samples from patients residing in the southern states and elsewhere in the USA, but no evidence of DNA from other Borrelia spp. was detected. Based on phylogenetic analysis of partial flagellin (flaB) gene sequences, strains that clustered separately with B. burgdorferi sensu stricto, Borrelia americana or Borrelia andersonii were associated with Lyme disease-like signs and symptoms in patients from the southern states, as well as from some other areas of the country. Strains most similar to B. burgdorferi sensu stricto and B. americana were found most commonly and appeared to be widely distributed among patients residing throughout the USA. The study findings suggest that human cases of Lyme disease in the southern USA may be more common than previously recognized and may also be caused by more than one species of B. burgdorferi sensu lato. This study provides further evidence that B. burgdorferi sensu stricto is not the only species associated with signs and/or symptoms consistent with Lyme borreliosis in the USA. © 2014 The Authors Printed in Great Britain.
News Article | December 22, 2016
INDIANAPOLIS, IN--(Marketwired - Dec 22, 2016) - hc1.com, inventor of the world's leading healthcare relationship management platform, has achieved another major growth milestone with more than 1,000 health system, lab, ambulatory, and post acute care sites across the globe relying on its healthcare CRM, live analytics, and secure collaboration capabilities to personalize the healthcare experience. Click to Tweet. About hc1.com hc1.com enables health systems, diagnostic labs, post acute care, and ancillary service organizations to personalize the healthcare experience for patients and providers throughout the entire care journey. hc1 has been adopted across more than 1,000 locations across the globe and has processed over 2.5 billion clinical transactions to date, generating more than 90 million consumer profiles. hc1.com enables a five star service experience for industry leaders including Cleveland Clinic, Alere, Sonic Healthcare and AmeriPath. The company has received accolades from Gartner Research, was named "Best Healthcare CRM" by Frost & Sullivan, and was featured in the KLAS Healthcare CRM Report 2016. To learn more about hc1, please visit www.hc1.com and follow us on Twitter @hc1_HRM, LinkedIn, and Facebook.
News Article | November 10, 2016
INDIANAPOLIS, IN--(Marketwired - Nov 10, 2016) - hc1.com, inventor of the world's leading healthcare relationship management platform, has earned status as an Advanced Technology Partner in the AWS Partner Network (APN) and also achieved Amazon Web Services (AWS) Healthcare Competency status. Click to Tweet. "Amazon Web Services serves as a powerful foundation for the hc1 Platform that enables us to deliver secure, highly scalable, highly available solutions that personalize the healthcare experience for our clients," said Brad Bostic, founder and CEO of hc1.com. Since its founding in 2011, hc1.com has rapidly expanded to become the world leader in healthcare relationship management with nearly 1,000 healthcare client sites. hc1 is a HIPAA-compliant, cloud-based platform that was built from the ground up to personalize the healthcare experience for providers, patients, and consumers. hc1 applies advanced data science to automatically generate comprehensive patient and provider profiles combining clinical and business data from any source to drive action, accountability, and personalized engagement. APN Advanced Technology Partners and AWS Healthcare Competency Partners undergo a rigorous certification process. AWS Healthcare Competency Partners have demonstrated success in building solutions to help healthcare providers and payers securely store, process, transmit, and analyze clinical information. "An important criteria for certification is real world success, which we demonstrated by showing how hc1 is widely adopted by healthcare professionals to drive efficiencies and growth within their organizations," said Laura Breedlove, senior vice president of technology at hc1.com. "AWS was able to hear firsthand how our customers benefit from the hc1 platform's scalability, data security, and compliance standards." About hc1.com hc1.com enables health systems, diagnostic labs, post acute care, and ancillary service providers to personalize the healthcare experience for patients and providers throughout the entire care journey. hc1 has been adopted across nearly 1,000 locations around the globe and has processed over 2.5 billion clinical transactions to date, generating more than 90 million consumer profiles enabling a five star service experience for industry leaders including Cleveland Clinic, Alere, Sonic Healthcare and AmeriPath. The company has received accolades from Gartner Research, was named "Best Healthcare CRM" by Frost & Sullivan, and was featured in the KLAS Healthcare CRM Report 2016. To learn more about hc1, please visit www.hc1.com and follow us on Twitter @hc1_HRM, LinkedIn, and Facebook.
Satelli A.,Texas Tech University Health Sciences Center |
Rao P.S.,Texas Tech University Health Sciences Center |
Thirumala S.,AmeriPath |
Rao U.S.,Texas Tech University Health Sciences Center |
Rao U.S.,1109 Amarillo Research Building
International Journal of Cancer | Year: 2011
Development of colorectal cancer (CRC) involves a series of genetic alterations with altered expression of proteins and cell signaling pathways. Here, we identified that galectin-4 (gal-4), a marker of differentiation, was down-regulated in CRC. The goal of this work was to determine the function of gal-4 in CRC. Toward this goal, the human colon biopsies and tissue microarrays containing a gradient of pathology were analyzed for gal-4 expression by immunohistochemistry. Cell proliferation, migration, motility, forced expression, knockdown, cell cycle and apoptosis assays were used to characterize gal-4 function. Immunohistochemistry identified that gal-4 expression was significantly down-regulated in adenomas and was essentially absent in invasive carcinomas. Forced expression of gal-4 in gal-4 -ve cells induced cell cycle arrest and retarded cell migration and motility. Further, gal-4 sensitized the cells to camptothecin-induced apoptosis. Gal-4 knockdown resulted in increased cell proliferation, migration and motility. Gal-4 was found to be associated with Wnt signaling proteins. Finally, gal-4 expression led to down-regulation of Wnt signaling target genes. This study demonstrates that loss of gal-4 is a common and specific event in CRC. This study also shows that gal-4 exhibits tumor suppressive effects in CRC cells in vitro. Through its ability to interact with and down-regulate the functions of Wnt signaling pathway, gal-4 reveals a new dimension in the control of the Wnt signaling pathway. Thus, gal-4 may prove to be an important molecule in understanding the biology of CRC. Copyright © 2010 UICC.
Performance differences between conventional smears and liquid-based preparations of thyroid fine-needle aspiration samples: Analysis of 47 076 responses in the college of american pathologists interlaboratory comparison program in non-gynecologic cytology
Fischer A.H.,University of Massachusetts Medical School |
Clayton A.C.,Mayo Medical School |
Bentz J.S.,Laboratory Medicine Consultants Ltd |
Wasserman P.G.,North Shore Long Island Jewish Health System |
And 3 more authors.
Archives of Pathology and Laboratory Medicine | Year: 2013
Context.-Controversy exists about whether thyroid fine-needle aspirates (FNAs) should be processed with conventional smears or liquid-based preparations (LBPs). Objective.-To compare the performance of conventional smears to LBPs for thyroid FNA slides circulated in the College of American Pathologists Interlaboratory Comparison Program in Non-Gynecologic Cytology. Design.-Participant responses for thyroid FNA slides were compared with the reference diagnosis at the level of 3 general diagnostic categories: negative, suspicious (which included only follicular and Hü rthle cell neoplasm), and malignant. For specific reference diagnoses of benign/ goiter and papillary thyroid carcinoma, the participants' specific diagnoses were analyzed and poorly performing slides were rereviewed. Results.-The 47 076 thyroid FNA slide responses, between 2001 and 2009, included 44 478 responses (94%) for conventional smears and 2598 responses (6%) for LBPs. For the general reference category negative, participant responses were discrepant in 14.9% of conventional smears compared with 5.9% for LBPs (P .001). The specific reference diagnosis of benign/goiter was misdiagnosed as a follicular neoplasm in 7.8% of conventional smears, comparedwith 1.3% of LBP. For the general reference category of malignant, participant responses were discrepant in 7.3% of conventional smears compared with 14.7% of LBPs (P .001). The specific reference diagnosis of papillary thyroid carcinoma was misdiagnosed as benign/goiter in 7.2% of LBPs, compared with 4.8% of conventional smears (p .001). Conclusions.-LBPs performed worse than conventional smears for cases with a reference diagnosis of papillary thyroid carcinoma. However, LBPs performed better than conventional smears for cases with a benign reference diagnosis. Specific features in thyroid FNAs that may improve the diagnostic accuracy of LBPs and conventional smears are described.
Neill J.S.A.,AmeriPath |
Lineaweaver W.C.,Joseph ill Burn And Reconstructive Center
American Journal of Clinical Pathology | Year: 2013
Objectives: To present the findings of the biological response and assimilation of bovine fetal collagen (BFC) as observed in biopsy specimens taken after implant. Methods: The biopsy specimens were from 9 patients with full-thickness skin wounds who received the BFC biomaterial in the first stage of a 2-stage reconstruction. Biopsy specimens were taken at the second stage of skin grafting from the wound margins. Results: The response to the BFC included neovascularization and infiltration of the collagen matrix with fibroblasts. The acellular matrix had the tinctural properties of devitalized tissue, which may be mistaken for coagulative necrosis if one is unaware of the biomaterial implant. Conclusions: The characteristics of BFC histology should be recognized by pathologists involved in patients treated for reconstruction and wound care. © American Society for Clinical Pathology.