Kim Y.-R.,Pukyong National University |
Kim E.-Y.,Pukyong National University |
Choi S.,Pukyong National University |
Hossain M.T.,Pukyong National University |
And 5 more authors.
Journal of Microbiology and Biotechnology | Year: 2012
The present study was aimed to investigate the effect of a probiotic, Enterococcus faecium, on the immune responses against infection with the marine fish pathogen Lactococcus garvieae in olive flounder (Paralichthys olivaceus). The immune responses were assessed by lysozyme activity, complement activity, protease activity, and expression of proinflammatory cytokines by RT-PCR. The lysozyme and complement activities were increased between 9 to 15 and 9 to 13 days, respectively, and antiprotease activity was slightly elevated after 5 days of probiotic treatment. The TNF-α and IL-1β expressions were observed from kidney and spleen. The results of this study reveal that E. faecium induces immune-responsible materials and protects olive flounder from lactococcosis. © The Korean Society for Microbiology and Biotechnology.
Kim E.-H.,Gachon University |
Hong H.,Gachon University |
Hong K.-S.,Gachon University |
Choi K.-S.,Gachon University |
And 4 more authors.
Journal of Food and Drug Analysis | Year: 2012
The incidence of inflammatory bowel diseases has increased during recent decades in Korea as well as Asian countries. Probiotics have been clinically administered to improve intestinal inflammation in inflammatory bowel disease (IBD). In this study, we identified that higher concentrations of probiotics called "Amanlac" probiotics protected intestinal tissues with the regulation of cytokine production and the improvement of intestinal injury of mice with dextran sodium sulfate (DSS)-induced colitis much better than commercial probiotics. "Amanlac" probiotics significantly ameliorated gross and pathological scores of colitis caused by DSS in a concentration- dependent manner based on the following mechanisms; inflammatory markers such as IL-1β, TNF α and COX-2, as well as MMPs and ICAM1 were significantly lower in colon tissues of probiotics-treated mice following DSS treatment compared with DSS-treated control mice, but the overall efficacy of "Amanlac" probiotics was significantly improved than conventional concentration of probiotics. In conclusion, the administration of higher concentrated probiotics helps to successfully maintain intestinal homeostasis, while also improving intestinal inflammation.
Jung I.S.,Pukyong National University |
Oh M.K.,AmBio Co. |
Cho Y.C.,AmBio Co. |
Kong I.S.,Pukyong National University
Applied Microbiology and Biotechnology | Year: 2011
Bifidobacterium longum grew at 65 L pilot scale of the membrane bioreactor (MBR), externally fitted with ceramic membrane (0.7 m 2). Cell mass at the MBR reached 22.18 gL -1 as dry cell weight in 12 h, which is 8.44 times higher than cell mass attained at the vial culture. The growth rate in the vial culture was μ=0.385 h - and at the batch culture was μ=1.13 h - in the exponential period and μ=0.31 h -1 in the stationary period. In the fed-batch mode was μ=1.102 h -1 for 6 h with inoculation and declined to μ=0.456 h -1 with feeding of feed medium. The growth rate at the MBR was μ=0.134 h -1. The number of viable cells was 6.01×10 12 cfu L -1 at the batch culture, but increased to 1.15×10 14 cfu L -1 at the MBR culture. The specific growth rate of viable cell number (colony-forming units per liter, per hour) improved by 6.01 times from the batch to the MBR culture. The wall shear stress mainly generated by the pump, and the membrane incorporated into the MBR was controlled during the cultivation at the MBR. The viability of B. longum declined to under 10% in the first 2 weeks of the 4-week stability test (40°C) as B. longum was exposed to over wall shear stress 713 Pa, but the viability improved to 30-40% in wall shear stress of 260 Pa or STR culture. The loss in the cell viability can be saved by managing with wall shear stress during the cultivation at the MBR. © 2011 Springer-Verlag.