Amano Enzyme Inc. | Date: 2016-11-03
Disclosed is a novel -galactosidase. Specifically disclosed are a -galactosidase derived from Bacillus circulans and a gene for the -galactosidase. The -galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.
Amano Enzyme Inc. | Date: 2016-10-19
The present invention addresses a problem of providing a lipase derived from a microorganism that is specific for short-chain to medium-chain fatty acids. A modified lipase is obtained by making a substitution in the amino acid sequence of a Candida cylindracea derived lipase, wherein the substitution is (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO: 1; or (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO: 1.
Amano Enzyme Inc. | Date: 2015-12-29
A novel method for improving an enzyme hydrolyzing an -1,6-glycosidic linkage is provided. A mutated enzyme is made by obtaining the amino acid sequence of a pullulanase enzyme having an amino acid sequence that is at least 95% identical to the amino acid sequence set forth in SEQ ID NO: 14; identifying an amino acid to be mutated in the pullulanase enzyme of step (1), wherein said amino acid to be mutated corresponds to the amino acid at position Phe^(476 )of SEQ ID NO: 2; constructing a mutated amino acid sequence by substituting the amino acid to be mutated with another amino acid or deleting the amino acid to be mutated, thereby making a mutated pullulanase enzyme having the mutated amino acid sequence that has increased affinity for pullulan and that hydrolyzes an -1,6-glycosidic linkage.
Amano Enzyme United States Ltd. and Amano Enzyme Inc. | Date: 2015-01-09
The present technology relates to an enzyme composition. The enzyme composition may be used to treat gluten intolerant subjects, including suffering from non-Celiac gluten intolerance and/or non-Celiac gluten sensitivity. The enzyme composition may also be used to reduce gluten exposure in certain individuals. For example, the enzyme composition may also be used as a prophylactic to reduce exposure to gluten oligopeptides.
Amano Enzyme Inc. | Date: 2016-03-08
The present invention is intended to provide a novel use of a maltotriosyl transferase. The present invention provides a method for producing rice cakes or noodles, including a step of heat-treating a dough containing maltotriosyl transferase thereby gelatinizing starch in the dough. The present invention also provides a method for producing an indigestible saccharide, including a step of allowing maltotriosyl transferase to act on a saccharide.
Amano Enzyme Inc. | Date: 2016-09-28
The object is to provide an -glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified -glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) is selected from a group of specific amino acids.
Amano Enzyme Inc. | Date: 2015-07-22
A protein having a novel saccharide oxidase activity capable of being subjected to various uses is provided. The present invention provides a protein having the following physicochemical characteristics:(1) effect: oxidizing a saccharide to produce a saccharic acid;(2) substrate specificity: acting on glucose, maltotriose, maltose, galactose, maltotetraose, lactose, and cellobiose; and,(3) [Km value of glucose]/[Km value of maltose] 1.
Amano Enzyme Inc. | Date: 2015-04-22
The present invention is intended to prove a technique useful for controlling the reaction of oxidoreductase, and to provide a reaction system allowing efficient conversion from carbon dioxide to formic acid, and an efficient methanol production system including the reaction system. The reverse redox reaction is selectively promoted by carrying out the reaction catalyzed by an oxidoreductase using an artificial electron carrier. The reaction system is used for the production of methanol.
Amano Enzyme Inc. | Date: 2015-10-27
Disclosed is a transglutaminase having excellent stability. Also disclosed is a process for producing the transglutaminase. Specifically disclosed is a stabilized transglutaminase, which has such a structure in which a pro-sequence peptide of transglutaminase is bound to a mature transglutaminase. Also specifically disclosed is a process for producing stabilized transglutaminase, which includes the steps of culturing a microorganism capable of producing transglutaminase under the conditions where transglutaminase can be produced; and separating and collecting matured transglutaminase having a pro-sequence peptide bound thereto from a culture medium.
Amano Enzyme Inc. | Date: 2016-09-07
The usefulness of -galactosidases derived from Bacillus circulans is further enhanced so that they can be applied to new applications. The present invention provides a modified -galactosidase in which one or more amino acids selected from the group consisting of proline at position 182 (P182), tyrosine at position 187 (Y187), serine at position 188 (S188), tryptophan at position 405 (W405), alanine at position 406 (A406), glutamine at position 407 (Q407), tyrosine at position 449 (Y449), threonine at position 483 (T483), serine at position 512 (S512), serine at position 531 (S531), serine at position 533 (S533), serine at position 534 (S534), asparagine at position 550 (N550), glutamine at position 551 (Q551), tryptophan at position 593 (W593), tyrosine at position 598 (Y598), proline at position 602 (P602), proline at position 604 (P604), tyrosine at position 609 (Y609), lysine at position 612 (K612), and tyrosine at position 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a -galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.