Amano Enzyme Inc.

Kakamigahara, Japan

Amano Enzyme Inc.

Kakamigahara, Japan
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Patent
Amano Enzyme Inc. | Date: 2017-01-23

A protein having a novel saccharide oxidase activity capable of being subjected to various uses is provided. The present invention provides a protein having the following physicochemical characteristics: (1) effect: oxidizing a saccharide to produce a saccharic acid; (2) substrate specificity: acting on glucose, maltotriose, maltose, galactose, maltotetraose, lactose, and cellobiose; and, (3) [Km value of glucose]/[Km value of maltose]1.


Patent
Amano Enzyme Inc. and Ise Foods. Inc. | Date: 2014-05-28

The present invention addresses the problem of providing an albumen hydrolysate that has emulsifiability, emulsion stability and heat coagulability. An albumen hydrolysate obtained by hydrolysis of albumen with a protease, wherein the dry weight of a precipitate formed by adding nine times the amount of 0.4 M trichloroacetic acid (TCA) to the albumen hydrolysate is 60% or more relative to the dry weight of albumen treated in the same manner.


Patent
Amano Enzyme Inc. | Date: 2017-06-28

The present invention has a purpose of providing a technique for increasing the heat resistance of a -galactosidase. According to the present invention, in a reference -galactosidase amino acid sequence which shows a 90% or more identity to the amino acid sequence of SEQ ID NO: 4, proline is substituted for one or more amino acids selected from the group consisting of the following amino acids: (1) an amino acid corresponding to lysine at position 166 of the amino acid sequence of SEQ ID NO: 4, (2) an amino acid corresponding to glycine at position 307 of the amino acid sequence of SEQ ID NO: 4, and (3) an amino acid corresponding to alanine at position 833 of the amino acid sequence of SEQ ID NO: 4.


Patent
Amano Enzyme Inc. | Date: 2016-11-03

Disclosed is a novel -galactosidase. Specifically disclosed are a -galactosidase derived from Bacillus circulans and a gene for the -galactosidase. The -galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.


Patent
Amano Enzyme Inc., Hatta and ISE Foods Inc. | Date: 2017-04-19

The present invention addresses the problem of providing an albumen hydrolysate that has emulsifiability, emulsion stability and heat coagulability. An albumen hydrolysate obtained by hydrolysis of albumen with a protease, wherein the dry weight of a precipitate formed by adding nine times the amount of 0.4 M trichloroacetic acid (TCA) to the albumen hydrolysate is 60% or more relative to the dry weight of albumen treated in the same manner.


Patent
Amano Enzyme Inc. | Date: 2016-10-19

The present invention addresses a problem of providing a lipase derived from a microorganism that is specific for short-chain to medium-chain fatty acids. A modified lipase is obtained by making a substitution in the amino acid sequence of a Candida cylindracea derived lipase, wherein the substitution is (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO: 1; or (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO: 1.


Patent
Amano Enzyme Inc. | Date: 2016-03-08

The present invention is intended to provide a novel use of a maltotriosyl transferase. The present invention provides a method for producing rice cakes or noodles, including a step of heat-treating a dough containing maltotriosyl transferase thereby gelatinizing starch in the dough. The present invention also provides a method for producing an indigestible saccharide, including a step of allowing maltotriosyl transferase to act on a saccharide.


Patent
Amano Enzyme Inc. | Date: 2016-09-28

The object is to provide an -glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified -glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) is selected from a group of specific amino acids.


Patent
Amano Enzyme Inc. | Date: 2015-07-22

A protein having a novel saccharide oxidase activity capable of being subjected to various uses is provided. The present invention provides a protein having the following physicochemical characteristics:(1) effect: oxidizing a saccharide to produce a saccharic acid;(2) substrate specificity: acting on glucose, maltotriose, maltose, galactose, maltotetraose, lactose, and cellobiose; and,(3) [Km value of glucose]/[Km value of maltose] 1.


Patent
Amano Enzyme Inc. | Date: 2016-09-07

The usefulness of -galactosidases derived from Bacillus circulans is further enhanced so that they can be applied to new applications. The present invention provides a modified -galactosidase in which one or more amino acids selected from the group consisting of proline at position 182 (P182), tyrosine at position 187 (Y187), serine at position 188 (S188), tryptophan at position 405 (W405), alanine at position 406 (A406), glutamine at position 407 (Q407), tyrosine at position 449 (Y449), threonine at position 483 (T483), serine at position 512 (S512), serine at position 531 (S531), serine at position 533 (S533), serine at position 534 (S534), asparagine at position 550 (N550), glutamine at position 551 (Q551), tryptophan at position 593 (W593), tyrosine at position 598 (Y598), proline at position 602 (P602), proline at position 604 (P604), tyrosine at position 609 (Y609), lysine at position 612 (K612), and tyrosine at position 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a -galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.

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