Prognostic value of microvessel density in tumor and peritumoral area as evaluated by CD31 protein expression and argyrophilic nucleolar organizer region count in endothelial cells in uterine leiomyosarcoma
Avdalyan A.,Altai Branch of Blokhin Russian Cancer Research Center |
Bobrov I.,Altai State Medical University |
Klimachev V.,Altai State Medical University |
Lazarev A.,Altai Branch of Blokhin Russian Cancer Research Center
Sarcoma | Year: 2012
The objective of this study was to investigate the prognostic value of microvessel density (MVD) in uterine leiomyosarcoma (LMS) and peritumoral area (PA) as evaluated by CD31 expression and argyrophilic nucleolar organizer region (AgNOR) count in endothelial cells. Tissue specimens from 66 patients with uterine LMS were examined. There were no significant differences in the mean MVD between tumor itself and the PA (P = 0.9); moreover, the MVD in the PA often exceeded that in the tumor. No correlation or significant differences were also found in the MVD between different grades of malignancy of LMS (r = 0.1; P = 0.07). The number of AgNORs in tumor endothelial cells was significantly higher in tumor vessels than in the peritumoral area (P < 0.005) and increased with the tumor grade. Analysis of the prognostic value of MVD in uterine LMS and PA showed that the density of tumor vessels was not an independent criterion, while the MVD in the PA affected 10-year survival to a significantly greater extent ( ρ2 = 27.5; P = 0.0003). The number of AgNORs also had an important effect on survival of LMS patients: when the threshold of 11.6 granules was exceeded, prognosis was significantly more unfavorable than that prior to exceeding the threshold. Copyright © 2012 Ashot Avdalyan et al.
Reference values of concentrations of matrix metalloproteinases-1, -2, -9 and the tissue inhibitor of matrix metalloproteinases (TIMP-1) in the amniotic fluid during physiological pregnancy and delivery
Korenovsky Y.V.,Altai State Medical University |
Remneva O.V.,Altai State Medical University
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | Year: 2016
The aim of this study was to determine reference values of matrix metalloproteinases (MMPs) MMP-1, MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in the amniotic fluid at the first stage of labor in physiological pregnancy. Eighty nine women at the first stage of term labor have been examined. Samples of amniotic fluid were taken at the first period of labor by vaginal amniotomy. Concentrations of MMP-1, MMP-2, MMP-9, and TIMP-1 have been investigated in amniotic fluid samples by ELISA kits. The following normal concentration ranges for MMP-1, MMP-2, MMP-9, TIMP-1, and ratios of concentrations of MMPs and TIMP-1 (MMP-1/TIMP-1, MMP-2/TIMP-1, MMP-9/TIMP-1) have been determined for amniotic fluid samples obtained during the first period of labor in physiological pregnancy. These included: MMP-1: 5.1–16.8 pg/mg of protein; MMP-2: 238.3–374.1 pg/mg of protein; MMP-9: 66.1–113.3 pg/mg of protein, TIMP-1: 4.7–13.6 pg/mg of protein, MMP-1/TIMP-1 ratio: 0.1–2.2, MMP-2/TIMP-1 ratio: 19.9–55.7; MMP-9/TIMP-1 ratio: 4.2–17.2. © 2016, Pleiades Publishing, Ltd.
Kuznetsova D.V.,Altai State Medical University |
Kulikov V.P.,Altai State Medical University
Blood Pressure Monitoring | Year: 2014
BACKGROUND: Arterial partial pressure alteration of CO2 (P aCO2) affects not only the cerebral blood flow velocity but also the systemic arterial blood pressure (BP). At the same time, BP can affect the cerebral blood flow. The objective of the present research is to study the impact of the PaCO2 level on cerebrovascular CO2 reactivity (CVRCO2) and BP as well as the impact of BP upon CVR CO2 alteration by hypercapnia and hypocapnia. MATERIALS AND METHODS: Cerebral blood flow velocity was recorded by means of transcranial Doppler in both middle cerebral arteries (MCAv left and right). The mean arterial pressure (MAP) was studied using the finger photoplethysmography method, arterial blood oxygen saturation was estimated by the pulse oximetry method, and end-tidal PCO2 (PETCO2) was measured with an infrared capnograph. After a recording of the reference values of all the parameters, all the volunteers underwent a rebreathing as well as a hyperventilation. RESULTS: At rest,PETCO2 was 33.6 (SD 3.1) mmHg. At rebreathing, MCAv increased at 38 mmHg PETCO2, MAP-at 43 mmHg PETCO2. By hyperventilation, MCAv decreased at 28 mmHg PETCO2, MAP-at 26 mmHg PETCO2. When PETCO2 reached 43 mmHg, CVRCO2 increased from 2.3 (SD 1.4) to 3.3 (SD 1.2)%/mmHg (P<0.01). When P ETCO2 decreased to 26 mmHg, CVRCO2 increased from-3.6 (SD 2.5) to-5.9 (SD 3.9)%/mmHg (P<0.01). CONCLUSION: Within the alteration of PETCO2 above 43 and under 26 mmHg, BP increased and decreased, respectively, leading to a change in CVRCO2. © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Korenovsky Y.V.,Altai State Medical University |
Remneva O.V.,Altai State Medical University
Biomedit︠s︡inskai︠a︡ khimii︠a︡ | Year: 2016
The aim of this study was to determine reference values of matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in the amniotic fluid at the first stage of labor in physiological pregnancy. 89 women at the first stage of term labor have been examined. Samples of amniotic fluid were taken at the first period of labor by vaginal amniotomy. Concentrations ofMMP-1, MMP-2, MMP-9, and TIMP-1 were investigated in amniotic fluid by ELISA kits. We have determined normal concentration ranges for MMP-1, MMP-2, MMP-9, TIMP-1, and ratios of concentrations of MMPs and TIMP-1 (MMP-1/TIMP-1, MMP-2/TIMP-1, MMP-9/TIMP-1) in the amniotic fluid at the first period of labor in physiological pregnancy. These included: MMP-1--5.1-16.8 pg/mg of protein, MMP-2--238.3-374.1 pg/mg of protein, MMP-9--66.1-113.3 pg/mg of protein, TIMP-1--4.7-13.6 pg/mg of protein, ratio of MMP-1/TIMP-1--0.1-2.2, ratio of MMP-2/TIMP-1--19.9-55.7, ratio of MMP-9/TIMP-1--4.2-17.2.Abstract available from the publisher.
Dudareva Yu.A.,Altai State Medical University |
Gurieva V.A.,Altai State Medical University
Human Ecology | Year: 2015
A prospective study of thyroid function in 112 women of the second generation descendants, ancestors of whom were in the area of radiation effects of the Semipalatinsk test site 29 August 1949. In retrospect, thyroid disease was studied in progenitors (233 women exposed to radiation) and parents - the descendants of the first generation (247 women). The control group included women not exposed to radiation and radiation lived outside the track. Retrospective analysis phase included archival documents and conducted with historical controls. Prospective phase included a study of the type "case - control". The state of the thyroid gland and its hormone profile, gorrmony pituitary. It was found that women who were in the area of radiation exposure, thyroid disease is more common, in contrast to the control group (8,2 ± 0,2) and (2,1 ± 0,3) %; p = 0.046, they are characterized by the presence of hyperthyroidism, hypothyroidism, postoperative (not excluded surgery for thyroid cancer), autoimmune thyroiditis, thyroid cancer. Among women, the first generation descendants of thyroid disease met by 68.3 % more than in the control group (p = 0.014), but as women of the second generation offspring. In women, the second-generation descendants greatly increased in the structure of the thyroid gland pathology share of autoimmune thyroiditis (11.6 %; p = 0.039), which was not observed in their parents and grandparents. Thus, the high incidence of thyroid disease is characterized by both women exposed to radiation, and their descendants in the first and second generation. © Northern State Medical University.
Barbaeva S.N.,Altai State Medical University |
Kulishova T.V.,Altai State Medical University
Voprosy kurortologii, fizioterapii, i lechebnoĭ fizicheskoĭ kultury | Year: 2016
We have studied stabilographic characteristics and their dynamics in the healthy children (n=30) and the patients suffering from juvenile cerebral palsy (JCP) in the form of spastic diplegia (n=99) after they had undergone the combined rehabilitation treatment with the use of various methods of electrical stimulation. The mean age of the children was 7.0±1.7 years. The patients with JCP included in the main group (n=45) received therapy with the application of electrical stimulation based on the AKorD apparatus while the patients with JCP comprising the group of comparison were treated with the use of the Mioritm 040 apparatus. Vertical stability of the schoolchildren was evaluated using the Stabilan-01-2 hardware system, once in the healthy children and twice (before and after the termination of rehabilitation) in the patients with JCP. A course of the rehabilitative treatment of the patients with JCP included in the main group resulted in a 24.6% and 15.8% reduction (p<0.05) of the statokinesiogram area in the tests with the open and closed eyes respectively. The visual control coefficient increased significantly. The patients of the comparison group experienced a marked reduction of the area of statokinesiogram in the tests with the open eyes (by 15,5% (р<0,05)) while the remaining characteristics of interest remained unaltered. It is concluded that the treatment of the children presenting with juvenile cerebral palsy with the use of the AKorD apparatus for electrical stimulation is more efficient for the maintenance of the vertical posture in comparison with the treatment based on the use of the Mioritm 040 apparatus.Abstract available from the publisher.
Tregub P.,Altai State Medical University |
Kulikov V.,Altai State Medical University |
Bespalov A.,Altai State Medical University
Pathophysiology | Year: 2013
Introduction: We studied the comparative efficacy of independent and combined effects of normobaric hypoxia (90. mmHg) and permissive hypercapnia (50. mmHg) in increasing the tolerance of rats to acute hypobaric hypoxia. Methods: We determined the time to loss of pose and life duration as a measure to assess the degree of tolerance of animals to hypobaric hypoxia by exposing them to an altitude of 11,500. m (barometric = 180. mmHg). Results: Exposure to hypercapnic hypoxia increased the tolerance to acute hypobaric hypoxia compared to exposure to normobaric hypoxia or permissive hypercapnia alone. Discussion: The positive effects of hypercapnia and hypercapnic hypoxia occurred after one exposure, and increasing the number of exposures proportionally increased the tolerance to acute hypobaric hypoxia. The effect of permissive hypercapnia on increasing the tolerance to acute hypobaric hypoxia was found to be significantly greater than that of exposure to normobaric hypoxia. Therefore, we propose that hypercapnia is the dominant factor in increasing tolerance to acute hypobaric hypoxia. Conclusion: Tolerance to acute hypoxia maximally increases in case of joint effect of normobaric hypoxia and permissive hypercapnia. © 2013 Elsevier Ireland Ltd.
Chumakova G.A.,Altai State Medical University |
Veselovskaya N.G.,Research Institute for Complex Issues of Cardiovascular Diseases
Russian Journal of Cardiology | Year: 2016
Obesity, especially visceral, is a chronic disorder with high risk of cardiometabolic complications. Precise diagnostics of visceral obesity (VO) is not easy, as most methods have benefits and restrictions for application as well. The article focuses on the opportunities and shortcomings of anthropometric, ultrasound methods, and magnet-resonance and computed tomography. We recommend to have broader application of simple and very much available method of direct VO diagnostics - echocardiography - by the measurement of epicardial fat layer. © 2016, Silicea-Poligraf. All rights reserved.
Shoikhet Ya.N.,Altai State Medical University |
Markin A.V.,Altai State Medical University
Terapevticheskii Arkhiv | Year: 2010
Aim. To determine the specific features of respiratory support in patients with obstructive sleep apnoea syndrome (OSAS) concurrent with chronic obstructive pulmonary disease (COPD). Subjects and methods. Thirty-six patients with OSAS concurrent with COPD were examined. External respiration function, apnoea/hypopnoea index, and arterial gas composition were determined in all the patients. The efficiency of continuous positive airway pressure (CPAP) therapy was evaluated in all the patients with the crossing-syndrome (CS). Results. The prevalence of CS among the patients with OSAS was 18.8%. The CS patients'need for bilevel positive airway pressure (BiPAP) therapy was 27.8%. These patients and those treated with CPAP had at baseline a daytime hypercapnia level of 50.7 ± 1.8 and 42.0 ±1.2 mm Hg (p = 0.005) and a nocturnal blood oxygen saturation level of 83.6 ± 1.3 and 88.8 ±0.9% (p -0.004), respectively. BiPAP therapy resulted in a 1.8-fold reduction in the desaturation index than did CPAP therapy-Conclusion. On choosing a respiratory support method in patients with OSAS, one should assess the presence of chronic lower airway obstruction, the degree and type of chronic respiratory failure, and the presence and magnitude of clinical, functional, and laboratory signs of alveolar hypoventilation. Bilevel lung ventilation is more effective in patients who have CS, daytime hypercapnia, and nocturnal hypoxemia uncorrected by CPAP therapy and who need high therapeutic pressure.
Konyaev S.V.,Asahikawa University |
Yanagida T.,Asahikawa University |
Ingovatova G.M.,Altai State Medical University |
Shoikhet Y.N.,Altai State Medical University |
And 4 more authors.
Parasitology International | Year: 2012
Mitochondrial haplotypes were determined for Echinococcus species infecting individuals diagnosed with alveolar echinococcosis (AE) and cystic echinococcosis (CE) at Altai State Medical University Hospital in Barnaul, Russia during 2008 to 2011. The nucleotide sequence of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was determined for 31 of 34 AE and 8 of 12 CE cases. All of the AE cases were confirmed to be caused by Asian type Echinococcus multilocularis, while CE cases were caused by Echinococcus granulosus sensu stricto (genotype G1) and Echinococcus canadensis (genotype G6). © 2012 Elsevier Ireland Ltd.