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Saint Petersburg, Russia

Gudkova A.,Pavlov Medical University | Kostareva A.,Heart Health | Sjoberg G.,Karolinska Institutet | Smolina N.,Heart Health | And 6 more authors.
Pediatric Cardiology | Year: 2013

Desmin cardiomyopathy is a rare cause of congestive heart failure. Its clinical manifestation in adulthood often is associated with conduction disorders and a neuromuscular phenotype. Only a few cases have been reported, with early manifestation in childhood mostly due to severe cardiomyopathy dilationand conduction abnormalities. However, the disease can result in the variety of clinical phenotypes, including hypertrophic, restrictive, and arrthythmogenic cardiomyopathy. This report describes the first case of desmin cardiomyopathy with early manifestation in adolescence and transformation of several clinical phenotypes over time, representing sufficient difficulties for the correct clinical diagnosis and treatment of the disease at an early stage. © 2012 Springer Science+Business Media, LLC.

Gurova M.M.,Belgorod national research University | Romanova T.A.,Belgorod national research University | Novikova V.P.,Almazov Federal Center for Heart | Avilova I.A.,Kursk State University
Biomedical Engineering | Year: 2015

Using gas chromatography–mass spectrometer for studying and evaluating the intestinal microflora in humans with chronic gastroduodenitis compared to traditional methods of diagnosis and microbiological analysis can significantly extend the range of the defined microbiota and its possible changes at different phases of the disease, thus enabling successful correction of microflora and suggesting prospective targeted treatment, followed by evaluation of its effectiveness. The composition and quantity of microorganisms in the intestinal wall of 340 patients aged 12 to 18 years were analyzed using gas chromatography–mass spectrometer in the acute phase, in remission, and at the end of treatment. © 2015 Springer Science+Business Media New York

Zemelko V.I.,Russian Academy of Sciences | Kozhucharova I.V.,Russian Academy of Sciences | Kovaleva Z.V.,Russian Academy of Sciences | Domnina A.P.,Russian Academy of Sciences | And 6 more authors.
Cell and Tissue Biology | Year: 2014

The ability of mesenchymal stem cells (MSCs) to differentiate into neuronal lineage determines the potential of these cells as a substrate for a cell replacement therapy. In this paper we compare the neurogenic potential of the MSCs from different donors, isolated from the bone marrow (BMSC), subcutaneous adipose tissue (AD MSC) and menstrual blood (eMSC). It was established that the native eMCSs, BMSCs and AD MSCs express neuronal marker β-III-tubulin with a frequency of 90, 50 and 14%, respectively. Also we showed that the eMSCs have a high endogenous level of brain-derived neurotrophic factor (BDNF), whereas the BMSCs and the AD MSCs are characterized by low basal BDNF levels. An induction of neuronal differentiation in the studied MSCs using differentiation medium containing B27 and N2 supplements, 5-azacytidine, retinoic acid, IBMX and dbcAMP induced changes in the cells morphology, the increase of β-III-tubulin expression, and the appearance of neuronal markers GFAP, NF-H, NeuN and MAP2. During the differentiation the BDNF secretion was significantly enhanced in the BMSCs and decreased in the eMSCs cultures. However, no correlation between the basal and induced levels of the neuronal markers expression in the studied MSCs has been established. © 2014 Pleiades Publishing, Ltd.

Shlyakhto E.V.,Almazov Federal Center for Heart
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2012

This article deals with peculiarities of development and clinical course of heart failure (HF) in diabetic patients, influence of diabetic cardiopathy on HF formation., role of genetic predictors of diabetes mellitus (DM) and HF formation, also the importance of treatment response predictors, the significance of a more personalized exposure in order to optimize treatment. The role of stationary and dynamic genomics was analyzed, especially molecular visualization that allows the earliest possible intervention. The article also includes examples of molecular visualization use in diagnosis of myocardial dysfunction, disease monitoring, and treatment efficacy assessment. Authors give an analysis of targeted treatment methods on the example of targeted delivery of medications to the target-organ (myocard). Discuss means of anti-ischemic myocardial protection, perspectives of metformin use in order to enhance efficacy of myocardial ischemic pre- and postconditioning mechanisms. Presented perspectives of stu^ of molecular and genetic mechanisms involved in the pathogenesis of HF in diabetic patients, in particular, study of key biological feahtres of stem cells, cell interactions, stem cell plasticity (in vitro direction of differentiation) and their paracrine fidnction evaluation. Given information about identification of genes with partly altered expression due to chronic exposure of mesenchymal stem cells to the high concentration of glucose, and upon decreased ability of mesenchymal stem cells ofproangiogenic factors with simultaneous increase of inflammatory markers production (IL8). In whole this article reviews modem state of HF in diabetic patients development mechanisms study with the use of molecular and genetic technologies, and ofperspectives of development of this area.

Dmitrieva R.I.,Almazov Federal Center for Heart | Anisimov S.V.,Russian Academy of Sciences
Cell and Tissue Biology | Year: 2013

Hematopoietic stem cells (HSCs) effectively and continuously replenish the full range of blood-cell populations. Bone-marrow and umbilical-cord blood stem-cell transplantation (SCT) restore hematopoiesis when used in various hematological and oncohematological disorders in adults and children. However, wider clinical application of effective SCT-based approaches is limited by the low number of primitive HSCs in the available biospecimens. Development of effective protocols of HSC expansion in vitro is therefore necessary. In this review, the notion of bone marrow hematopoiesis is discussed as a complex cellular system and a comparative analysis of various methods for HSC expansion in vitro is provided. The review is illustrated by our own data supporting application of various feeder-cell types for human HSC expansion in vitro. © 2013 Pleiades Publishing, Ltd.

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