Clinical Research Center for Allergy and Rheumatology

Japan

Clinical Research Center for Allergy and Rheumatology

Japan
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Kono A.,Tokai University | Brameier M.,Leibniz Institute for Primate Research | Roos C.,Leibniz Institute for Primate Research | Suzuki S.,Tokai University | And 9 more authors.
Journal of Immunology | Year: 2014

The common marmoset (Callithrix jacchus) is a NewWorld monkey that is used frequently as a model for various human diseases. However, detailed knowledge about the MHC is still lacking. In this study, we sequenced and annotated a total of 854 kb of the common marmoset MHC region that corresponds to the HLA-A/G/F segment (Caja-G/F) between the Caja-G1 and RNF39 genes. The sequenced region contains 19 MHC class I genes, of which 14 are of the MHC-G (Caja-G) type, and 5 are of the MHC-F (Caja-F) type. Six putatively functional Caja-G and Caja-F genes (Caja-G1, Caja-G3, Caja-G7, Caja-G12, Caja-G13, and Caja-F4), 13 pseudogenes related either to Caja-G or Caja-F, three non-MHC genes (ZNRD1, PPPIR11, and RNF39), two miscRNA genes (ZNRD1-AS1 and HCG8), and one non-MHC pseudogene (ETF1P1) were identified. Phylogenetic analysis suggests segmental duplications of units consisting of basically five (four Caja-G and one Caja-F) MHC class I genes, with subsequent expansion/deletion of genes. A similar genomic organization of the Caja-G/F segment has not been observed in catarrhine primates, indicating that this genomic segment was formed in New World monkeys after the split of New World and Old World monkeys. The Journal of Immunology, 2014, 192: 3239-3246. © Copyright 2014 by The American Association of Immunologists, Inc. All rights reserved.


Kitamura F.,Tokyo Metropolitan Institute of Medical Science | Kitamura N.,Clinical Research Center for Allergy and Rheumatology | Mori A.,Clinical Research Center for Allergy and Rheumatology | Tatsumi H.,National Hospital Organization | And 5 more authors.
International Archives of Allergy and Immunology | Year: 2010

Background: Among several C-terminal binding proteins (CtBPs), friend of GATA (FOG) has been implicated in the down-regulation of GATA-3-mediated Th2 cell differentiation. Here we investigated the role of CtBP2 in Th1 and Th2 cytokine expression in human T cells. Methods: CtBP2 was introduced into human peripheral CD4+ T cells by a lentiviral transduction system. Subsequently, the expression of Th1 and Th2 cytokine mRNA was determined by quantitative real-time RT-PCR. Results: CtBP2 significantly suppressed stimulation-induced expression of IL-4, IL-5 and IL-13 in human T cells. However, IFN-γ expression was not affected by the introduction of CtBP2. Conclusion: CtBP2 selectively down-regulates Th2 cytokines, therefore it is a potential target for the treatment of allergic diseases. Copyright © 2010 S. Karger AG, Basel.

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