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Patiwael J.A.,Section of Allergology | Vullings L.G.J.,Agricultural Occupational Health and Safety Advisory Services STIGAS | De Jong N.W.,Section of Allergology | Van Toorenenbergen A.W.,Erasmus University Rotterdam | And 2 more authors.
International Archives of Allergy and Immunology | Year: 2010

Background: Employees in strawberry greenhouses are highly exposed to several (potential) allergenic agents. However, no occupational allergy in this branch has been described before. First, the presence of work-related allergic symptoms in strawberry workers was explored. Second, we aimed to prove the concept that an IgE-mediated allergy could be responsible for work-related symptoms. To test the possibility of an IgE response secondary to cross-reactivity to birch or grass pollen, inhibition experiments were performed. Methods: First, a questionnaire survey concerning work-related allergic symptoms among strawberry workers in the Netherlands was carried out. Second, 3 workers with work-related symptoms were investigated in detail. Skin tests, serum-specific IgE tests with home-made extracts of strawberry pollen and other possible allergenic agents of the strawberry greenhouse environment were executed. Furthermore, immunoblots and nasal provocations with strawberry pollen extract were performed. In addition, inhibition experiments were performed. Results: 29 of 75 questionnaire respondents (38.7%) reported work-related symptoms. Sensitization to strawberry pollen was found in skin tests in all 3 employees with work-related symptoms. ELISA and immunoblotting with strawberry pollen showed positive results in 2 employees. Birch and grass pollen failed to inhibit IgE binding to strawberry pollen in 1 of 2 employees. Partial inhibition was seen in the second employee. Nasal provocation validated clinically relevant allergy to these pollens in 2 of 3 subjects. Conclusions: Allergic symptoms attributable to the workplace are present among a proportion of strawberry greenhouse employees. An IgE-mediated occupational allergy to strawberry pollen may contribute to these symptoms. © 2009 S. Karger AG, Basel. Source

Ciprandi G.,Semeiotica e Metodologia Medica i | De Amici M.,University of Pavia | Tosca M.,Allergy Center | Marseglia G.,University of Pavia
International Archives of Allergy and Immunology | Year: 2010

Background:Allergic rhinitis (AR) is characterized by inflammation sustained by dysregulated immune response. T-regulatory cells are involved in AR pathogenesis, mainly producing IL-10 and transforming growth factor (TGF)-β. Indeed, there is a functional and allergen-specific defect of T-regulatory cells in AR. However, there are no data about the influence of allergen exposure on TGF-β serum levels. Therefore, the aim of this preliminary study was to evaluate TGF-β serum levels in patients with seasonal AR. Patients were evaluated either outside the pollen season and after 1 preseasonal sublingual immunotherapy (SLIT) course (38 subjects) or during the pollen season (57 subjects). Methods:All patients were allergic to Parietaria and/or grasses alone. TGF-β was measured by a commercially available kit. Symptoms, drug use and eosinophils were evaluated.Serum allergen-specific IgG and IgA levels were also measured by the ELISA method. Results:TGF-β serum levels were significantly lower in patients evaluated outside the pollen season in comparison with the other 2 situations. SLIT induced the significantly highest TGF-β serum levels. There was a significant negative relationship between TGF-β and eosinophils in patients after SLIT. IgG and IgA levels were higher in SLIT-treated patients. Conclusion: This preliminary study provides evidence that TGF-β serum levels are significantly dependent on allergen exposure. © 2009 S. Karger AG, Basel. Source

Malinauskiene L.,Allergy Center | Malinauskiene L.,Skane University Hospital | Zimerson E.,Skane University Hospital | Bruze M.,Skane University Hospital | And 2 more authors.
Contact Dermatitis | Year: 2013

Background Simultaneous contact allergies to Disperse Orange 1, 4-nitroaniline and p-aminodiphenylamine (PADPA), as well as to other disperse azo dyes and to p-phenylenediamine (PPD), have been reported. Cross-reactivity is one of the possible explanations for simultaneous reactions between PPD and disperse azo dyes. Some metabolites from the azo reduction of these disperse azo dyes could be sensitizers, as human skin bacteria produce azo reductases. Objectives To investigate the sensitizing capacity of Disperse Orange 1, PADPA and 4-nitroaniline, and the cross-reactivity between these substances and Disperse Yellow 3, its potential metabolites from azo reduction (4-aminoacetanilide and 2-amino-p-cresol), and PPD. Method The guinea-pig maximization test was used. Results It was found that both Disperse Orange 1 and PADPA are strong sensitizers and cross-react with each other. We were unable to sensitize guinea-pigs with 4-nitroaniline tested in equimolar concentrations to Disperse Orange 1. Conclusions The results indicate that patients sensitized primarily to Disperse Orange 1 will also react to PADPA, which can be found mainly in hair dyes. PPD, 4-nitroaniline, 4-aminoacetanilide, 2-amino-p-cresol and Disperse Yellow 3 did not show any cross-reactivity with Disperse Orange 1 or PADPA. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Source

Fujisawa T.,Allergy Center
Japanese Journal of Allergology | Year: 2015

Children are thought to have high plasticity in the immune system. Thus, it is expected that allergen immunotherapy for children may have higher efficacy than adults and has real disease-modifying potential to allergic diseases. For clinical efficacy in ameliorating allergic symptoms, however, current systematic reviews have not shown any superior effect in children, partly because of less number of studies in this age group. On the other hand, preventive effects against asthma inception in children with allergic rhinitis and sensitization "spreading" to new allergens have been reported, implicating that allergen immunotherapy has a potential to stop "allergy march" in children. To make the therapy more effective, it is preferable to start it as early as possible, hopefully in infancy. But it is still difficult at present because of relatively frequent adverse events and invasive manner of administering allergen extracts for young children. We need safer and more effective methods in performing immunotherapy for children. © 2015 Japanese Society of Allergology. Source

Lee C.C.,China Medical University at Taichung | Ho H.,Graduate Institute of Immunology | Lee K.T.,Institute of Microbiology and Biochemistry | Jeng S.T.,National Taiwan University | And 4 more authors.
Cellular and Molecular Immunology | Year: 2011

In clinical therapy, the amount of antigen administered to achieve oral tolerance for allergic diseases is large, and the cost is a major consideration. In this study, we used tobacco plants to develop a large-scale protein production system for allergen-specific immunotherapy, and we investigated the mechanisms of oral tolerance induced by a transgenic plant-derived antigen. We used plants (tobacco leaves) transgenic for the Dermatophagoides pteronyssinus 2 (Der p2) antigen to produce Der p2. Mice received total protein extract from Der p2 orally once per day over 6 days (days 0-2 and days 6-8). Mice were also sensitized and challenged with yeast-derived recombinant Der p2 (rDer p2), after which the mice were examined for airway hyper-responsiveness and airway inflammation. After sensitization and challenge with rDer p2, mice that were fed with total protein extracted from transgenic plants showed decreases in serum Der p2-specific IgE and IgG1 titers, decreased IL-5 and eotaxin levels in bronchial alveolar lavage fluid, and eosinophil infiltration in the airway. In addition, hyper-responsiveness was also decreased in mice that were fed with total protein extracted from transgenic plants, and CD4 CD25 Foxp3 regulatory T cells were significantly increased in mediastinal and mesenteric lymph nodes. Furthermore, splenocytes isolated from transgenic plant protein-fed mice exhibited decreased proliferation and increased IL-10 secretion after stimulation with rDer p2. The data here suggest that allergen-expressing transgenic plants could be used for therapeutic purposes for allergic diseases. © 2011 CSI and USTC. All rights reserved. Source

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