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Isbary G.,Allergology and Environmental Medicine
NATO Science for Peace and Security Series A: Chemistry and Biology

Infected chronic wounds are both socioeconomic and medical problem. Cold atmospheric plasma (CAP) has already proven its efficacy in killing bacteria on agar plates but also the first prospective randomized controlled trial in patients. As an add-on therapy CAPs proved a highly significant decrease in bacterial load in 5 min plasma-treated wounds (34%, p < 10 -6, n = 291, 36 patients) in comparison with wounds that received only standard wound care. This reduction is found in all kinds of germs, even multiresistant ones. Two minutes of plasma treatment led to a significant reduction in bacterial load as well (40%, p < 0.016, n = 70, 14 patients). The treatment is very well tolerated and no side effects occurred until now (in total more than 2,000 treatments in over 220 patients). The results of this study revealed the potential of atmospheric argon plasma treatment as a new approach to kill bacteria in terms of mutiresistancy. With the same CAP device other dermatologic diseases were treated successfully, e.g. Hailey-Hailey disease. New plasma devices using surrounding ambient air have not only greater bactericidal but also virucidal properties. These devices may herald a new era in public, personal, pet, and food hygiene, same as in decontamination. Investigations of human compatibility are promising. © 2012 Springer Science+Business Media B.V. Source

Maisch T.,University of Regensburg | Shimizu T.,Max Planck Institute for Extraterrestrial Physics | Isbary G.,Allergology and Environmental Medicine | Heinlin J.,University of Regensburg | And 5 more authors.
Applied and Environmental Microbiology

Candida albicans is one of the main species able to form a biofilm on almost any surface, causing both skin and superficial mucosal infections. The worldwide increase in antifungal resistance has led to a decrease in the efficacy of standard therapies, prolonging treatment time and increasing health care costs. Therefore, the aim of this work was to demonstrate the applicability of atmospheric plasma at room temperature for inactivating C. albicans growing in biofilms without thermally damaging heatsensitive materials. This so-called cold atmospheric plasma is produced by applying high voltage to accelerate electrons, which ionize the surrounding air, leading to the production of charged particles, reactive species, and photons. A newly developed plasma device was used, which exhibits a large plasma-generating surface area of 9 by 13 cm (117 cm2). Different time points were selected to achieve an optimum inactivation efficacy range of ≥ 3 log10 to 5 log10 reduction in CFU per milliliter, and the results were compared with those of 70% ethanol. The results obtained show that contact-free antifungal inactivation of Candida biofilms by cold atmospheric plasma is a promising tool for disinfection of surfaces (and items) in both health care settings and the food industry, where ethanol disinfection should be avoided. © 2012, American Society for Microbiology. Source

Tronnier M.,Hildesheim GmbH | Semkova K.,Medical University-Sofia | Wollina U.,Allergology and Environmental Medicine | Tchernev G.,Sofia University
Wiener Medizinische Wochenschrift

In faired skinned population the incidence of melanoma is rapidly increasing. Beside environmental factors (UV-exposure) certainly other reasons for the observed "melanoma epidemic" have to be discussed. For diagnostic procedure classical histopathology is accompanied by immunohistochemistry and more recently molecular techniques. For therapy new modalities are available which - after many years of frustrating search for new drugs - are now able to prolong both disease/progression free and overall survival. © 2013 Springer-Verlag Wien. Source

Klampfl T.G.,Max Planck Institute for Extraterrestrial Physics | Klampfl T.G.,TU Munich | Isbary G.,Allergology and Environmental Medicine | Shimizu T.,Max Planck Institute for Extraterrestrial Physics | And 6 more authors.
Applied and Environmental Microbiology

Physical cold atmospheric surface microdischarge (SMD) plasma operating in ambient air has promising properties for the sterilization of sensitive medical devices where conventional methods are not applicable. Furthermore, SMD plasma could revolutionize the field of disinfection at health care facilities. The antimicrobial effects on Gram-negative and Gram-positive bacteria of clinical relevance, as well as the fungus Candida albicans, were tested. Thirty seconds of plasma treatment led to a 4 to 6 log10 CFU reduction on agar plates. C. albicans was the hardest to inactivate. The sterilizing effect on standard bioindicators (bacterial endospores) was evaluated on dry test specimens that were wrapped in Tyvek coupons. The experimental D23°C values for Bacillus subtilis, Bacillus pumilus, Bacillus atrophaeus, and Geobacillus stearothermophilus were determined as 0.3 min,0.5 min, 0.6 min, and 0.9 min, respectively. These decimal reduction times (D values) aredistinctly lower than D values obtained with other reference methods. Importantly, the high inactivation rate was independent of the material of the test specimen. Possible inactivation mechanisms for relevant microorganisms are briefly discussed, emphasizing the important role of neutral reactive plasma species and pointing to recent diagnostic methods that will contribute to a better understanding of the strong biocidal effect of SMD airplasma. © 2012, American Society for Microbiology. Source

Jeon J.,Max Planck Institute for Extraterrestrial Physics | Rosentreter T.M.,Max Planck Institute for Extraterrestrial Physics | Li Y.,Max Planck Institute for Extraterrestrial Physics | Isbary G.,Allergology and Environmental Medicine | And 4 more authors.
Plasma Processes and Polymers

The composition of the gas for the Surface Micro-Discharge (SMD) plasma system is changed by applying variety of binary mixtures with oxygen, nitrogen and argon. Gram-negative Escherichia coli and gram-positive Enterococcus mundtii bacteria strains are inoculated on agar and exposed to the plasma using different gas mixture ratios either with a fixed applied voltage or with a fixed dissipated power. No bacterial reduction is observed in the absence of oxygen. Higher bactericidal efficacy is observed with higher oxygen fraction in the gas composition for the plasma. A threshold of the oxygen fraction is found where the bacterial reduction does not change much. The strong correlation between the bactericidal efficacy and the ozone concentration measured by optical absorption spectroscopy suggests that ozone generated by the SMD plasma is the major agent for the bacterial inactivation under the investigated conditions. The reduction of Escherichia coli and Enterococcus mundtii strains by the Surface Micro-Discharge (SMD) plasma is investigated using O2/N 2/Ar binary gas mixtures. Relationships between the bactericidal properties and the ozone concentration generated by the SMD plasma are studied. The results show no bacterial reduction without the admixture of oxygen and a strong correlation between the bacterial reduction and ozone concentration. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

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