Ramachandran E.,Allahabad Agricultural Institute Du |
Ramachandran E.,JK AgriGenetics Ltd. |
Bhattacharya S.K.,JK AgriGenetics Ltd. |
John S.A.,Allahabad Agricultural Institute Du |
And 3 more authors.
Journal of Biotechnology | Year: 2011
The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3 gene showed earliness of 4-15 days variations in flowering and maturity. The transgenic lines were confirmed by kanamycin leaf paint assay, GUS assay and PCR. Among 67 transgenic lines, the event-10 showed profuse branching, event-24 showed abnormal growth and the remaining events exhibited single erect phenotype. In addition, the event-24 produced no flower and this might be due to the positional effect of PTM3 gene integration. Southern blot analysis performed for event-10, 24 and 48 showed distinct single copy integrations of PTM3 gene cassette. GUS assay performed using various plant parts of event-10 showed constitutive expression of the transgene. In view of cotton breeding, among all the events, the event-10 was found to be phenotypically significant with earliness of 12 days in flowering and 15 days in maturity and yield enhancement of 27%. In addition, the event-10 showed no square dropping and allowed the plants to bear more number of bolls. Based on these results, event-10 was chosen to carryout the inheritance study of expressed characters in the progeny. © 2011 Elsevier B.V.
Anamika,Allahabad Agricultural Institute Du |
Simon S.,Allahabad Agricultural Institute Du
Archives of Phytopathology and Plant Protection | Year: 2011
Aloe vera dry rot caused by Alternaria alternata, is one of the most serious fungal diseases affecting the commercial cultivation of aloe vera in North India. Control of this disease through chemical is quite expensive and not ecofriendly. The plant extracts as biopesticdes act as a vital components for the management of this disease. Evaluation of some plant extracts was carried out against A. alternata in laboratory conditions. The extracts were prepared at 5% and 10% concentration and were evaluated through inhibition in radial growth (food poison technique) and spores (conidia) germination (hanging drop technique) against A. alternata. Neem leaf extract gave 58.6% inhibition in radial growth and 56.5% in spore germination at 10% concentration followed by Ocimum sanctum which was found effective and gave 54.7% inhibition in radial growth and 50.4% in spore germination over control. © 2011 Taylor & Francis.