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Sidorova T.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Miroshnichenko D.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Dolgov S.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Tjukavin G.,All Russian Research Institute of Vegetable Breeding and Seed Production
Acta Horticulturae | Year: 2013

Transgenic carrot (Daucus carota L.) plants expressing a thaumatin II protein were obtained by Agrobacterium-mediated transformation. Embryogenic calli of 'Nantskaya 4' were transformed by disarmed supervirulent agrobacterial strain CBE21 harboring the pBIThau35 plasmid. The presence of the thauII gene was confirmed by PCR, and the expression of the thaumatin II protein in primary transgenic plants was confirmed by Western blot analysis. Positive T0 carrot plants were self-pollinated and T1, T2 and T 3 seeds were produced. Resulted seeds were further selected for the antibiotic resistance and the inheritance and expression of thauII gene was evaluated by PCR and Western blot analysis. From these, five groups of T 2 and T3 progenies of two T0 lines were analyzed for the enhanced tolerance to the foliar and root pathogen Fusarium avenaceum. Among tested carrot plants, both T2 and T3 transgenic progenies of 40-61 line showed a fairly strong resistance against pathogen.


Sidorova T.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Miroshnichenko D.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Dolgov S.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Tjukavin G.,All Russian Research Institute of Vegetable Breeding and Seed Production
Acta Horticulturae | Year: 2015

A construct containing two chimeric genes, Cauliflower mosaic virus 35s - (bar) and nopaline synthase-neomycin phosphotransferase (nptII), was introduced into carrot cultivar 'Nantskaya 4′ using Agrobacterium tumefaciens-mediated transformation procedure. A number of transgenic carrot plants showing the stable integration of the bar gene were produced from embryogenic callus culture. Most of the obtained transgenic plants showed complete herbicide resistance at 0.05-0.50% Basta. Thus, introduction of the bar gene into a carrot cultivar can allow efficient production of herbicide-resistant transgenic carrot with commercial potential. © 2015 ISHS.


Karavaev V.A.,Moscow State University | Gunar L.E.,Moscow State University | Myakinkov A.G.,Moscow State University | Gins M.S.,All Russian Research Institute of Vegetable Breeding and Seed Production | And 3 more authors.
Biophysics (Russian Federation) | Year: 2012

Treatment of barley plants with a supercritical fluid extract of amaranth led to an increase in parameter (FM - FT)/FT of the leaf slow fluorescence induction curve. Barley treated with the extract showed higher productivity and better crop indices. © 2012 Pleiades Publishing, Ltd.


Krivenkov L.V.,All Russian Research Institute of Vegetable Breeding and Seed Production | Dobrutskaya E.G.,All Russian Research Institute of Vegetable Breeding and Seed Production | Mukhortov V.U.,All Russian Research Institute of Vegetable Breeding and Seed Production
Acta Horticulturae | Year: 2012

Introduction of new crops and extension of the assortment of vegetables are important tasks of breeding programs in Russia. Ten accessions of artichoke (Cynara scolymus L.) were tested during three years (2005-2008) under the condition of Moscow region. The high variability between accessions of artichoke was recorded for many traits related to growth and development of plant. According to the test results, the sources of early ripeness, short-growing, green and violent color of the head were selected. Out of the ten accessions tested, the Vp-48 ('Green Globe') was the most promising for yield, early ripeness, and optimal habit for cultivation in the condition of temperate climate of Russia. © ISHS.


Domblides E.A.,All Russian Research Institute of Vegetable Breeding and Seed Production | Domblides A.S.,All Russian Research Institute of Vegetable Breeding and Seed Production | Zayachkovskaya T.V.,All Russian Research Institute of Vegetable Breeding and Seed Production | Bondareva L.L.,All Russian Research Institute of Vegetable Breeding and Seed Production
Russian Journal of Genetics: Applied Research | Year: 2016

Identification of cytoplasm types by molecular markers enables us to simplify the parent pair selection for the production of hybrids based on cytoplasmic male sterility (CMS). To determine cytoplasm types, 40 accessions of the Brassicaceae family (Brassicaceae Burnett) were analyzed using 22 pairs of PCR primers published earlier. Seven different cytoplasm types (Ogura, Ogu-NWSUAF, nap, pol, cam, rad, and ole) were revealed in collection accessions by standard and multiplex PCR. The sequencing of PCR products, which differentiated cytoplasmic sterility types, confirmed the presence of three CMS-associated genes, orf138, orf222, and orf224, in the mitochondrial DNA of the studied accessions. Two genes, orf138 and orf222, were revealed in the mtDNA of a sterile napa cabbage accession that corresponded to the Ogu-NWSUAF type. All accessions with the Ogura cytoplasm had a 417-bp fragment completely (100%) identical to the mitochondrial gene orf138 type А (nine types of gene orf138 designated from A to I have been identified earlier). The only exception was the F1 accession of a white cabbage variety Tekila, which carried a new allelic variant of the gene orf138 containing a 39-bp deletion typical for type F of this gene and two nonsynonymous substitutions, A → T and G → A, at positions 95 and 99, respectively. © 2016, Pleiades Publishing, Ltd.

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