Notova S.V.,Orenburg State University |
Duskaeva A.H.,Orenburg State University |
Miroshnikov S.A.,All Russian Research Institute of Beef Cattle Breeding Russia |
Duskaev G.K.,All Russian Research Institute of Beef Cattle Breeding Russia |
Sizova E.A.,Orenburg State University
Biosciences Biotechnology Research Asia | Year: 2015
Article presents the results of research on composition of elements in skeletal muscles and hair of lab rats given the influence of food factors. Research was conducted on male Wistar rats from 2-month age (n=51). Within the record period animals were divided into 3 groups depending on the consumed diet. Animals of the control group (ÃŠ) were fed with full-value semisynthetic diet within 60 days (basic diet (BD). Animals of the I test group consumed semisynthetic diet I (SD I), consisting of mixture of major feed (50%), instant food (IF) (50%) and water, animals of the II group â€" semisynthetic diet II (SD II), consisting of mixture of major feed, instant food and carbonated soft drink (each 50%). Elements of the researched biological substrates and food products were analyzed by atomic emission and mass spectroscopy with inductive-connected argon plasma using devices Optima 2000 DV and ELAN 9000 (PerkinElmer, USA). It was established that in the experimental conditions the elemental composition of hair changed in accordance with changes in pool of the most elements in muscular tissue. It was found out that concentrations of phosphorus, potassium, sodium, iron and cobalt increased in hair (1.1 - 8.7 times) and calcium, zinc and manganese increased (1.3 â€" 1.4 times) with decrease in common pool of elements in muscular tissue.