All Russian Plant Quarantine Center

Bykovo, Russia

All Russian Plant Quarantine Center

Bykovo, Russia

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Blyummer A.G.,All Russian Plant Quarantine Center
Russian Entomological Journal | Year: 2017

Uranembia rivkusi gen. et sp.n. (Embioptera: Paedembiidae) is described from the Kyzylkum Desert in Uzbekistan. © Russian Entomological Journal, 2017.


Drenova N.V.,All Russian Plant Quarantine Center | Kharchenko A.A.,All Russian Plant Quarantine Center | Kuznetsova A.A.,All Russian Plant Quarantine Center | Balandina M.B.,All Russian Plant Quarantine Center | And 4 more authors.
Acta Horticulturae | Year: 2014

Fire blight was first detected in the Russian Federation (RF) in 2003. Since 2007, in the course of annual monitoring conducted by the Russian NPPO, Erwinia amylovora has been detected in 13 regions located in the European part of the Russian Federation. By areas of their detection, the outbreaks may be grouped as follows: outbreaks in Kaliningrad Region (the western enclave of Russia), the Central Black Earth Area (Voronezh, Tambov, Belgorod, Lipetsk and Penza regions), Lower Volga area (Samara, Saratov and Volgograd regions), as well as Stavropol and Krasnodar Krais and the Northern Caucasus (Kabardino-Balkaria and Karachaevo-Cherkessia). These areas vary in terms of their climatic conditions and agricultural production practices. This accounts for significant differences in the severity of damage caused by the fire blight in these areas. The first study of five E. amylovora strains from Kaliningrad, Voronezh, Tambov, Volgograd regions and Kabardino-Balkaria indicated that all of these strains were related (except for the strain from Kaliningrad). Currently, the study on the biology, origin, distribution and potential damage caused by the pest is being conducted. The bacteriological laboratories of the All-Russian Plant Quarantine Center (VNIIKR, the main research institution of the Russian NPPO) are situated in eight regions of the Russian Federation. Detection and identification of the fire blight is performed in compliance with VNIIKR's Technical Standard based on the EPPO Standard PM 20(1). Furthermore, a FLASH-PCR commercial kit for detection of E. amylovora developed in Russia has been validated and recommended as a simple and reliable screening and confirming test. © 2014, International Society for Horticultural Science. All rights reserved.


Sheveleva A.,Moscow State University | Ivanov P.,Moscow State University | Prihodko Y.,All Russian Plant Quarantine Center | James D.,Canadian Food Inspection Agency | Chirkov S.,Moscow State University
Plant Disease | Year: 2012

In studying the distribution and genetic diversity of Plum pox virus (PPV) in Russia, over a dozen new PPV isolates belonging to the strain Winona (PPV-W) were identified by immunocapture reverse-transcription polymerase chain reaction with the PPV-W-specific primers 3174-SP-F3/3174-SP-R1. Isolates were detected in two geographically distant regions of European Russia (Northern Caucasus and Moscow regions) in naturally infected plum (Prunus domestica), blackthorn (P. spinosa), Canadian plum (P. nigra), and downy cherry (P. tomentosa). The new PPV-W isolates were shown to be serologically related but not identical by triple-antibody sandwich enzyme-linked immunosorbent assay and Western blotting analysis using the monoclonal antibody (MAb) 5B-IVIA and MAbs specific to the N-terminal epitopes of PPV-W isolate 3174. Analysis of nucleotide and deduced amino acid sequences of the (C-ter)NIb-(N-ter)CP genome region indicate great genetic diversity among isolates, with phylogenetic analysis revealing seven clades. Isolates P1 and P3 found in plum in the south of Russia clustered closely with the putative ancestral PPV-W isolate LV-145bt from Latvia, while isolate 1410-7 found in P. nigra in Moscow appears to be closely related to the Canadian isolate W3174. The data obtained indicate wide dissemination of PPV-W isolate in stone fruit in the European part of the former USSR. © 2012 The American Phytopathological Society.


Cuccodoro G.,Museum dHistoire Naturelle | Kurbatov S.A.,All Russian Plant Quarantine Center | Pereira De Oliveira L.,University Estadual Of Santa Cruz | Santana Fonseca M.,University Estadual Of Santa Cruz
Psyche | Year: 2012

The types of the species of the Goniacerine genus of Pselaphinae Paragoniastes Comellini are revised. Paragoniastes parki Comellini, 1979, is synonymized with P. besucheti Comellini, 1979 (P. besucheti = P. parki syn. nov.), and P. uesci Cuccodoro & Kurbatov sp. nov. is described from the Brazilian state of Bahia. These taxa are described, illustrated, and keyed. Additional characters pertaining to the genus are given. © Copyright 2012 Giulio Cuccodoro et al.


Panferov V.G.,RAS A.N. Bach Institute of Biochemistry | Safenkova I.V.,RAS A.N. Bach Institute of Biochemistry | Varitsev Y.A.,Ag Lorch All Russian Potato Research Institute | Drenova N.V.,All Russian Plant Quarantine Center | And 3 more authors.
Talanta | Year: 2016

Ralstonia solanacearum is a dangerous and economically important pathogen of potatoes and other agricultural crops. Therefore, rapid and sensitive methods for its routine diagnostics are necessary. The aim of this study was to develop a rapid control method for R. solanacearum with a low limit of detection (LOD) based on a lateral flow immunoassay (LFIA) with silver enhancement. To minimize the LOD, the membrane type, antibody amount for conjugation with gold nanoparticles, conjugate concentration and antibody concentration in the analytical zone were optimized. Silver enhancement was used to decrease the LOD of the LFIA. For silver enhancement, release fiberglass membranes with pre-absorbed silver lactate and hydroquinone were placed on the analytical zone, and a drop of silver lactate was added. The LFIA with silver enhancement was found to be 10-fold more sensitive (LOD 2×102 CFU/mL; 20 min) in comparison with the common analysis (LOD 2×103 CFU/mL; 10 min). The specificity of the developed LFIA was studied using different strains of R. solanacearum (54 samples) and other widespread bacterial pathogens (18 samples). The LFIA detected all tested strains, whereas non-specific reactions were not observed. The developed tests were used for the control of bacteria in extracts of infected and non-infected potato tubers, and the quantitative analysis results (based on the densitometry of line colouration) were confirmed by ELISA with a correlation coefficient equal to 0.965. © 2016 Elsevier B.V. All rights reserved.


PubMed | RAS A.N. Bach Institute of Biochemistry, All Russian Plant Quarantine Center and Ag Lorch All Russian Potato Research Institute
Type: | Journal: Analytical and bioanalytical chemistry | Year: 2016

Early detection of potato infections is essential for effective disease management. The aim of this study was to develop a lateral flow immunoassay (LFIA) for rapid detection of a serious potato disease, potato blackleg, caused by Dickeya dianthicola and Dickeya solani. Polyclonal antibodies specific to different strains of Dickeya were obtained from rabbits after immunization with bacterial cells of D. dianthicola and D. solani. Enzyme-linked immunosorbent assay testing with use of a wide range of bacterial species showed that the polyclonal antibodies detect closely related strains of D. dianthicola and D. solani. Cross-reactivity with widespread pathogenic bacteria (nine species) and saprophytes of healthy potato plants was not detected. The LFIA based on the obtained antibodies and gold nanoparticles with average diameter of 20nm was developed. Under optimized conditions, the LFIA method enabled the analysis of potato extracts within 10min, with a visual limit of detection of 110


PubMed | RAS A.N. Bach Institute of Biochemistry, All Russian Plant Quarantine Center and Ag Lorch All Russian Potato Research Institute
Type: | Journal: Talanta | Year: 2016

Ralstonia solanacearum is a dangerous and economically important pathogen of potatoes and other agricultural crops. Therefore, rapid and sensitive methods for its routine diagnostics are necessary. The aim of this study was to develop a rapid control method for R. solanacearum with a low limit of detection (LOD) based on a lateral flow immunoassay (LFIA) with silver enhancement. To minimize the LOD, the membrane type, antibody amount for conjugation with gold nanoparticles, conjugate concentration and antibody concentration in the analytical zone were optimized. Silver enhancement was used to decrease the LOD of the LFIA. For silver enhancement, release fiberglass membranes with pre-absorbed silver lactate and hydroquinone were placed on the analytical zone, and a drop of silver lactate was added. The LFIA with silver enhancement was found to be 10-fold more sensitive (LOD 210(2) CFU/mL; 20 min) in comparison with the common analysis (LOD 210(3) CFU/mL; 10 min). The specificity of the developed LFIA was studied using different strains of R. solanacearum (54 samples) and other widespread bacterial pathogens (18 samples). The LFIA detected all tested strains, whereas non-specific reactions were not observed. The developed tests were used for the control of bacteria in extracts of infected and non-infected potato tubers, and the quantitative analysis results (based on the densitometry of line colouration) were confirmed by ELISA with a correlation coefficient equal to 0.965.


Kolesnichenko K.A.,Moscow State University | Bush M.G.,All Russian Plant Quarantine Center
Zoologicheskii Zhurnal | Year: 2015

The intraspecific structure of Mellicta ambigua (Ménétriés 1859) is revised and ranges of its subspecies are specified. M. ambigua kurmaevi ssp. n. from northern Yakutia is described and the lectotype of M. ambigua ambigua is designated. Contentious issues related to the identification of typical localities and authorship of the taxon kenteana (Staudinger 1897) are resolved.


Shamilov A.S.,All Russian Plant Quarantine Center
EPPO Bulletin | Year: 2012

Until 2005 disinfestation for plant quarantine purposes in the Russian Federation was mainly based on methyl bromide. Methyl bromide is effective against most important insect pests and is also effective against a wide range of nematodes. However, because of its contribution to stratospheric ozone depletion its use has not been permitted in developed countries since 2005, and in developing countries phase out will take place by 2015. In contrast, phosphine remains widely used, even though many insects have developed resistance to it. Alternative methods are also used such as refrigeration, controlled modified atmospheres, methyl iodide and sulfuryl fluoride. The present paper discusses the history of disinfestation in the USSR and describes the new methods which were developed and used as phytosanitary treatments in the Russian Federation. The techniques presented in this paper are the result of many years of scientific and practical work and the search for alternatives is ongoing. © 2012 The Author. Journal compilation © 2012 OEPP/EPPO.

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