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Gagkaeva T.,All Russian Institute of Plant Protection VIZR | Gavrilova O.P.,All Russian Institute of Plant Protection VIZR | Yli-Mattila T.,University of Turku | Loskutov I.G.,Saint Petersburg State University
Euphytica | Year: 2013

Fusarium head blight (FBH) is a serious disease of cultivated oats (Avena sativa L.). The objective of this study was to screen a set of oat genotypes from the VIR Avena sativa germplasm collection for the FHB resistance. Of the 155 screened genotypes 42 % belonged to both hulled and naked cultivars, 55 % were landraces and the rest were breeding lines. Screening nurseries were planted at two locations: in the Northwestern Russia in 2007-2008 with artificial inoculation by F. sporotrichioides strains and in the Russian Far East in 2009 (natural infections). The resistance data were based on three different parameters: (a) percentage of grain showing evidence of Fusarium damage, (b) the DNA content of the trichothecene-producing Fusarium species and (c) the trichothecene mycotoxin accumulation; the sum of which permitted identification of genotypes with multicomponent resistance to FHB. The highest degrees of resistance were observed in the naked cvs. Iymay (k-11014, China) and Numbat (k-14851, Australia) and by the hulled cv. Kuromi (k{cyrillic}-11632, Japan). The most resistant landraces were the hulled oats originating from the Asian region (k-2513, k-6963, k-7766, and k-8479). Considerable similarity was observed between the resistance reactions of oat genotypes to the Fusarium disease under different environmental conditions. The identified resistant accessions may serve as crossing partners in oat breeding efforts. © 2013 Springer Science+Business Media Dordrecht.


Pavela R.,Czech Republic Crop Research Institute | Zabka M.,Czech Republic Crop Research Institute | Kalinkin V.,The branch office of All Russian Institute of Plant Protection VIZR | Kotenev E.,Stavropol Research Antiplague Institute | And 3 more authors.
Plant Protection Science | Year: 2013

In 2010 and 2011, the efficiency of azadirachtin, applied as systemic trunk injections in the trunks of Platanus sp., was tested against Corythucha ciliata. Azadirachtin in the doses of 0.1 and 0.05 g of active ingredient per cm of diameter at breast height was applied in April. It was found that after application of both doses, the count of C. ciliata in plane-tree leaves significantly decreased. In 2010 and 2011, the average counted number of C. ciliata individuals in trees treated with the dose 0.1 g a.i./cm of dbh was 12.9 and 4.9, respectively, and 29.1 and 6.5 individuals, respectively, in trees treated with the dose 0.05 g a.i./cm of dbh, in the control it was 152.3 and 105.8 individuals, respectively. © 2011 Czech Academy of Agricultural Sciences.


Yli-Mattila T.,University of Turku | Ward T.J.,1815 iversity St | O'Donnell K.,1815 iversity St | Proctor R.H.,1815 iversity St | And 6 more authors.
International Journal of Food Microbiology | Year: 2011

Production of type A trichothecenes has been reported in the closely related species Fusarium langsethiae and F. sporotrichioides. Here, we characterized a collection of Fusarium isolates from Siberia and the Russian Far East (hereafter Asian isolates) that produce high levels of the type A trichothecene T-2 toxin and are similar in morphology to the type A trichothecene-producing F. langsethiae, and to F. poae which often produces the type B trichothecene nivalenol. The Asian isolates possess unique macroscopic and microscopic characters and have a unique TG repeat in the nuclear ribosomal intergenic spacer (IGS rDNA) region. In Asian isolates, the TRI1-TRI16 locus, which determines type A versus type B trichothecene production in other species, is more similar in organization and sequence to the TRI1-TRI16 locus in F. sporotrichioides and F. langsethiae than to that in F. poae. Phylogenetic analysis of the TRI1 and TRI16 gene coding regions indicates that the genes in the Asian isolates are more closely related to those of F. sporotrichioides than F. langsethiae. Phylogenetic analysis of the beta-tubulin, translation elongation factor, RNA polymerase II and phosphate permease gene sequences resolved the Asian isolates into a well-supported sister lineage to F. sporotrichioides, with F. langsethiae forming a sister lineage to F. sporotrichioides and the Asian isolates. The Asian isolates are conspecific with Norwegian isolate IBT 9959 based on morphological and molecular analyses. In addition, the European F. langsethiae isolates from Finland and Russia were resolved into two distinct subgroups based on analyses of translation elongation factor and IGS rDNA sequences. Nucleotide polymorphisms within the IGS rDNA were used to design PCR primers that successfully differentiated the Asian isolates from F. sporotrichioides and F. langsethiae. Based on these data, we formally propose that the Asian isolates together with Norwegian isolate IBT 9959 comprise a novel phylogenetic species, F. sibiricum, while the two subgroups of F. langsethiae only represent intraspecific groups. © 2011 Elsevier B.V.


Yli-Mattila T.,University of Turku | Gavrilova O.,All Russian Institute of Plant Protection VIZR | Hussien T.,University of Turku | Gagkaeva T.,All Russian Institute of Plant Protection VIZR
Journal of Plant Pathology | Year: 2015

Fusarium langsethiae is an European species, while Fusarium sibiricum is mainly distributed in northern Asia. Morphological characters alone do not allow a clear separation of F. langsethiae from F. sibiricum. The long TG repeat in the ribosomal IGS region is the only DNA sequence that has been used to design the species-specific primer pair CNL 12/PulvIGSr for the identification of F. sibiricum isolates. Another way to identify F. sibiricum is to use a combination of F. sporotrichioides- and F. Langsethiaespecific primer pairs. The aim of this study was to find new ways for identifying and discriminating F. Langsethiae from F. sibiricum. The paper also reports the first record of F. sibiricum from Iran, outside northern Asia and Norway, and the first isolation of F. Langsethiae, an European pathogen, from western Siberia. Thus, the distribution of F. sibiricum and F. Langsethiae may be much larger in Eurasia than what is currently known. © 2015, Journal of Plant Pathology. All Rights Reserved.


Kononenko G.P.,All Russian Research Institute for Veterinary Sanitation | Burkin A.A.,All Russian Research Institute for Veterinary Sanitation | Gavrilova O.P.,All Russian Institute of Plant Protection VIZR | Gagkaeva T.Y.,All Russian Institute of Plant Protection VIZR
Agricultural and Food Science | Year: 2015

The quality of grasses and legumes crops used for animal feed for the combined determination of fungal species and mycotoxin measurements were explored in samples collected from the fields of stock-farms located in North-western part of Russia. The occurrence of aflatoxin B1, alternariol, citrinin, cyclopiazonic and mycophenolic acids, deoxynivalenol, diacetoxyscirpenol, emodin, ergot alkaloids, fumonisins, ochratoxin A, PR-toxin, roridin A, sterigmatocystin, T-2 toxin and zearalenone, were determined using enzyme-linked immunosorbent assay. The most common fungi were Cladosporium, followed by Phoma, Alternaria, Aureobasidium, Acremonium and Fusarium. Invariably high incidence of alternariol, cyclopiazonic acid, emodin and ergot alkaloids was detected in all forage types. The contribution of the fusariotoxin contamination appeared to be less significant. The grasses and grass-legume mixtures before the first cut for the year were similar in terms of high incidence of sterigmatocystin. The plants were allowed to regrow, and the complex of four regularly occurring components was supplemented with fumonisins, mycophenolic acid, and ochratoxin A. © 2015, MTT Agrifood Research Finland. All rights reserved.

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