All Russia Research Institute of Vegetable Breeding and Seed Production

Moscow oblast, Russia

All Russia Research Institute of Vegetable Breeding and Seed Production

Moscow oblast, Russia
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Sidorova K.K.,RAS Institute of Cytology and Genetics | Levko G.D.,All Russia Research Institute of Vegetable Breeding and Seed Production | Shumny V.K.,RAS Institute of Cytology and Genetics
Russian Journal of Genetics: Applied Research | Year: 2013

The number of root nodules and nitrogen-fixation rate (judged from nitrogenase activity) were studied in nine varieties of five annual vetchling species: Lathyrus sativus, L. tingitanus, L. chloranthus, L. ochrus, and L. odoratus. All vetchling varieties were inferior to pea Pisum sativum in their nitrogen-fixation rate. A variation in nodulation pattern (nodule by number, size, and location) on roots and nitrogen-fixation rate among species and varieties was detected. With regard to nitrogen fixation, the Alisa and Nokturn varieties of sweet pea (L. odoratus) and Anna and Pink Flamingo of Tangier pea (L. tingitanus) deserve special attention. © 2013 Pleiades Publishing, Ltd.


Budylin M.V.,Moscow State University | Kan L.Y.,All Russia Research Institute of Vegetable Breeding and Seed Production | Romanov V.S.,All Russia Research Institute of Vegetable Breeding and Seed Production | Khrustaleva L.I.,Moscow State University
Russian Journal of Genetics | Year: 2014

Genomic in situ hybridization (GISH) was used for a chromosomal composition study of the later generations of interspecific hybrids between A. cepa L. and A. fistulosum L., which are relatively resistant to downy mildew (peronosporosis). GISH revealed that F2 hybrids, which did not produce seeds, were triploids (2n = 3x = 24) with 24 chromosomes and possessed in their complements 16 chromosomes of A. fistulosum L. and eight chromosomes of A. cepa L. or eight chromosomes of A. fistulosum L. and 16 chromosomes of A. cepa L. The advanced F5 hybrid, which produced few seeds, was amphidiploid with 32 chromosomes. BC1F5 hybrid was triploid with eight chromosomes of A. fistulosum L. and 16 chromosomes of A. cepa L., which did not produce seeds. BC2 (BC1F5) plant was amphidiploid that possessed 4 recombinant chromosomes and produced few seeds. GISH results point to 2n-gametes formation in macro- and microsporogenesis of the hybrids. The mechanism of 2n-gametes formation and the possibility of apomixes events in the backcrossing progeny are discussed. © 2014 Pleiades Publishing, Inc.


Shumilina D.V.,All Russia Research Institute of Vegetable Breeding and Seed Production | Shmykova N.A.,All Russia Research Institute of Vegetable Breeding and Seed Production | Bondareva L.L.,All Russia Research Institute of Vegetable Breeding and Seed Production | Suprunova T.P.,All Russia Research Institute of Vegetable Breeding and Seed Production
Biology Bulletin | Year: 2015

The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2–2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige-Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3 g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants. © Pleiades Publishing, Inc., 2015.

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