All Russia Research Institute of Oil Crops VNIIMK

Krasnodar, Russia

All Russia Research Institute of Oil Crops VNIIMK

Krasnodar, Russia
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Demurin Ya.N.,All Russia Research Institute of Oil Crops VNIIMK | Tronin A.S.,All Russia Research Institute of Oil Crops VNIIMK | Pikhtyareva A.A.,All Russia Research Institute of Oil Crops VNIIMK
Helia | Year: 2016

The genetic stocks of SURES-1 and SURES-2 showed complete tolerance to tribenuron-methyl. No resistance were obtained after observation on 200,000 plants of four VNIIMK breeding lines grown on an experimental field with total area of 5 ha treated with Express at 1x rate. Potential frequency of a dominant gene for tolerance to tribenuron-methyl was estimated less than 5 × 10-6. Moreover screening both of 700 plants of 39 inbred lines of the genetic collection and 800 plants of M2 four breeding lines did not reveal any resistance. Tolerance to tribenuron-methyl in F1 and F2 in the crosses of SURES-1 and SURES-2 with VNIIMK breeding lines was controlled by one dominant gene. All recessive homo-zygous hybrid plants of sur sur genotype in the cross were dead with phytotoxicity index of 9 after Express treatment at any rate of tribenuron-methyl from 0.5x to 4x. Heterozygous hybrid plants of Sur sur genotype showed no injury symptoms at 0.5x rate and increasing level of phytotoxicity index of 4, 6 and 7 for 1x, 2x and 4x rates correspondingly. Dominant homozygous hybrid plants of Sur Sur genotype possessed no injury symptoms from 0.5x to 2x rates whereas the phytotoxicity index of 4 was observed at the 4x rate. Consequently there was no difference between dominant homozygote Sur Sur and heterozygote Sur sur with phytotoxicity index of 0 at 0.5x rate. Allelic interaction in a heterozygote Sur sur can vary from complete to partial dominance in accordance with increasing rate of tribenuron-methyl treatment estimating with the phenotypic scale of phytotoxicity index.


Demurin Y.,All Russia Research Institute of Oil Crops VNIIMK | Borisenko O.,All Russia Research Institute of Oil Crops VNIIMK | Bochkarev N.,All Russia Research Institute of Oil Crops VNIIMK
Helia | Year: 2010

Pleiotropic effect of a high pamitic (p) and high oleic (Ol) mutations in sunflower seed oil was investigated in VK580 near-Isogenic lines in field conditions. It was found that the p mutation decreased plant height by 41 cm (32%), number of leaves by 4 (14%) and the period of emergence-flowering by 2 days (4%). The Ol mutation significantly increased plant height by 4 cm (3%). In addition, relationship between inheritance of high palmitic mutation and plant height in the case of selfing of F 2 plants was observed in the cross VK850 (p) × VK508 (Ol). The correlation coefficient was r=-0.54 with linear equation y=154.49-1.95x. The average plant height of the high palmitic homozygotes in the segregated F 2 population was lower and estimated at 109 cm as compared with 138 cm in the other plants (LSD 05=3 cm). Thus, a negative effect of the high palmitic mutation on plant height was proved beyond doubt. A question remains whether this phenomenon relates to a gene linkage or true pleiotropy.


Demurin Y.,All Russia Research Institute of Oil Crops VNIIMK | Borisenko O.,All Russia Research Institute of Oil Crops VNIIMK
Helia | Year: 2011

Development of genetic collection is considered to be an essential part of genetic resources of cultivated sunflower. The VNIIMK genetic collection of identified alleles determining oleic acid content in the seed oil consists of about 50 constant inbred lines. Fourteen of them are in a core collection. The general range of this trait varies from 20 to 92%. These inbred lines correspond to four main phenotypic classes: low, conventional, mid and high oleic. The normal line RIL100 was shown to contain a high oleic mutation Ol in hypostatic condition. The mid oleic LG27 averagely 67% does not contain the high oleic mutation in genetic background. The high oleic LG 26 with relatively low content of oleic acid, about 86%, possesses resistance to suppressor's actions.

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