Gulevich A.Y.,All Russia Research Institute of Genetics and Selection of Industrial Microorganisms |
Skorokhodova A.Y.,All Russia Research Institute of Genetics and Selection of Industrial Microorganisms |
Morzhakova A.A.,All Russia Research Institute of Genetics and Selection of Industrial Microorganisms |
Antonova S.V.,All Russia Research Institute of Genetics and Selection of Industrial Microorganisms |
And 3 more authors.
Applied Biochemistry and Microbiology | Year: 2012
Anaerobic biosynthesis of 1-butanol from glucose is investigated in recombinant Escherichia coli strains which form butyryl-CoA using the heterologous enzyme complex of clostridia or as a result of a reversal in the action of native enzymes of the fatty acid β-oxidation pathway. It was revealed that when the basic pathways of acetic and lactic acid formation are inactivated due to deletions of the ackA, pta, poxB, and ldhA genes, the efficiency of butyryl-CoA biosynthesis and its reduced product, i. e., 1-butanol, by two types of recombinant stains is comparable. The limiting factor for 1-butanol production by the obtained strains is the low substrate specificity of the basic CoA-dependent alcohol/aldehyde dehydrogenase AdhE from E. coli to butyryl-CoA. It was concluded that, in order to construct an efficient 1-butanol producer based on a model strain synthesizing butyryl-CoA as a result of reversed action of fatty acid β-oxidation enzymes, it is necessary to provide intensive formation of acetyl-CoA and enhanced activity of alternative alcohol and aldehyde dehydrogenases in the cells of a strain. © 2012 Pleiades Publishing, Ltd.
Zakalyukina Y.V.,Moscow State University |
Golichenkov M.V.,Moscow State University |
Brovkina O.I.,All Russia Research Institute of Genetics and Selection of Industrial Microorganisms |
Putyatina T.S.,Moscow State University
Moscow University Biological Sciences Bulletin | Year: 2014
A comparative analysis of the abundance and biodiversity of actinomycete communities isolated from living ants Lasius niger and Formica cunicularia, as well as their anthills, has been carried out for the first time. The number of actinomycetes detected in L. niger ants is close to the number of actinomycetes in their anthills and one order is higher than that for F. cunicularia. Actinomycete communities of anthills and the intact soil are characterized by a high diversity, equitability, absence of severe dominants, and the presence of common species and differ by number and the range of species. Biodiversity of actinomycetes associated with living ants is considerably lower than in their nests and the surrounding soil and the range of actinomycetes is specific for both the species of ants. © 2014, Allerton Press, Inc.