All Russia Research Institute of Agricultural Microbiology

Saint Petersburg, Russia

All Russia Research Institute of Agricultural Microbiology

Saint Petersburg, Russia
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Osipova M.A.,Saint Petersburg State University | Dolgikh E.A.,All Russia Research Institute of Agricultural Microbiology | Lutova L.A.,Saint Petersburg State University
Russian Journal of Developmental Biology | Year: 2011

In recent years, the role of WOX genes encoding homeodomain transcription factors in the development of the apical meristem of shoots and roots has been actively investigated. However, the role of WOX genes in the control of the cell proliferation in other meristem types is poorly studied. In our work, we have studied the role of the WOX5 gene in the development of the meristem in nitrogen-fixing nodules developing on the roots of legumes in a symbiosis with rhizobia. We have shown that the WOX5 gene is involved in the development of the nodule meristem in legumes, have quantitatively evaluated the gene's expression at different nodule formation stages, and have studied the localization of its expression using a construct containing the WOX5 promoter fused with a reporter gene. The role of the WOX5 transcription factor in the nodule organogenesis and its possible interaction with the hormonal system in the course of the nodule development has been discussed. © 2011 Pleiades Publishing, Ltd.


Provorov N.A.,Saint Petersburg State University of Information Technologies, Mechanics and Optics | Onishchuk O.P.,All Russia Research Institute of Agricultural Microbiology | Yurgel S.N.,Washington State University | Kurchak O.N.,All Russia Research Institute of Agricultural Microbiology | And 4 more authors.
Russian Journal of Genetics | Year: 2014

Using the example of N2-fixing legume-rhizobial symbiosis, we demonstrated that the origin and evolution of bacteria symbiotic for plants involve: (i) the formation of novel sym gene systems based on reorganizations of the bacterial genomes and on the gene transfer from the distant organisms; (ii) the loss of genes encoding for functions that are required for autonomous performance but interfere with symbiotic functions (negative regulators of symbiosis). Therefore, the construction of effective rhizobia strains should involve improvement of sym genes activities (for instance, nif, fix, and dct genes encoding for nitrogenase synthesis or for the energy supply of N2 fixation), as well as the inactivation of negative regulators of symbiosis identified in our lab (eff genes encoding for the transport of sugars and the production of polysaccharides and storage compounds, as well as for oxidative-reductive processes). © 2014, Pleiades Publishing, Inc.


Teijeiro R.G.,Lancaster University | Dodd I.C.,Lancaster University | Elphinstone E.D.,Myerscough College | Safronova V.I.,All Russia Research Institute of Agricultural Microbiology | Belimov A.A.,All Russia Research Institute of Agricultural Microbiology
Acta Horticulturae | Year: 2011

Many plant growth promoting rhizobacteria (PGPR) containing the enzyme ACC deaminase (ACCd) stimulate root (and shoot) growth by decreasing plant production of the plant hormone ethylene, which is often inhibitory to growth. The potential for incorporating ACCd-containing PGPR in vegetable transplant production was evaluated in a Lactuca sativa (lettuce)-Variovorax paradoxus 5C-2 combination. Effects of V. paradoxus 5C-2 were restricted to early growth stages: primary root growth was enhanced (15%) in filter paper culture and three weeks after seedling transplant to the field, biomass accumulation was greater (37%) in drying soil. These effects were not apparent in longer-term field trials, or in greenhouse pot trials, but did not seem to be related to the level of colonization of V. paradoxus 5C-2 on lettuce roots. Future experiments with this plant:bacterial combination should occur in unfavourable growing conditions known to stimulate ethylene evolution and inhibit growth.


Ivanova E.A.,All Russia Research Institute of Agricultural Microbiology | Kutovaya O.V.,Dokuchaev Soil Science Institute | Tkhakakhova A.K.,Dokuchaev Soil Science Institute | Chernov T.I.,Dokuchaev Soil Science Institute | And 4 more authors.
Eurasian Soil Science | Year: 2015

The taxonomic structure of microbiomes in aggregates of different sizes from typical chernozems was investigated using sequencing of the 16S rRNA gene. The aggregate fractions of <0.25, 2–5, and >7 mm obtained by sieving of the soil samples at natural moisture were used for analysis. The highest prokaryote biomass (bacteria, archaea) was determined in the fractions <0.25 and aggregates 2–5 mm; the bacterial and archaeal biomass decreased in the following series: fallow > permanent black fallow > permanent winter wheat. The greatest number of fungi was recorded in the fraction <0.25 mm from the soils of the permanent black fallow and in all the studied aggregate fractions in the variant with permanent wheat. The system of agricultural use affected more significantly the structure of the prokaryote community in the chernozem than the size of aggregate fractions did. The most diverse microbial community was recorded in the soil samples of the fallow; the statistically significant maximums of the Shannon diversity indices and indices of phylogenetic diversity (PD) were recorded in the fractions <0.25 and 2–5 mm from the fallow soil. On the whole, the fine soil fractions (<0.25 mm) were characterized by higher diversity indices in comparison with those of the coarser aggregate fractions. © 2015, Pleiades Publishing, Ltd.


Kutovaya O.V.,Dokuchaev Soil Science Institute | Lebedeva M.P.,Dokuchaev Soil Science Institute | Tkhakakhova A.K.,Dokuchaev Soil Science Institute | Ivanova E.A.,All Russia Research Institute of Agricultural Microbiology | Andronov E.E.,All Russia Research Institute of Agricultural Microbiology
Eurasian Soil Science | Year: 2015

For the first time, the composition of microbiomes in the biological crust (AKL) horizons of extremely arid desert soils (Aridic Calcisols) developed from saline and nonsaline alluvial deposits in the Ili Depression (eastern Kazakhstan) was analyzed. To describe the diversity of microorganisms in the soil samples, a novel method of pyrosequencing (Roche/454 Life Sciences) was applied. It was shown that bacteria from the Proteobacteria, Actinobacteria, Firmicutes, Verrucomicrobia, Acidobacteria, and Bacteroidetes phyla predominate in all the samples; these are typical representatives of the microbiome of soil crusts. A distinctive feature of the extremely arid soils is the high contribution of cyanobacteria (25–30%) to the total DNA. In the soils developed from saline sediments, representatives from the Rubrobacteraceae, Streptococcaceae, and Caulobacteraceae families and from the Firmicutes phylum predominated. In the soils developed from nonsaline gypsiferous deposits, bacteria from the class of Acidobacteria, subgroup Gp3, of the Methylobacteriaceae family and the class of Subdivision 3 from the Verrucomicrobia phylum predominated. © 2015, Pleiades Publishing, Ltd.


Muntyan V.S.,All Russia Research Institute of Agricultural Microbiology | Cherkasova M.E.,All Russia Research Institute of Agricultural Microbiology | Andronov E.E.,All Russia Research Institute of Agricultural Microbiology | Simarov B.V.,All Russia Research Institute of Agricultural Microbiology | Roumiantseva M.L.,All Russia Research Institute of Agricultural Microbiology
Russian Journal of Genetics | Year: 2016

Genomes of 184 Sinorhizobium meliloti native isolates were studied to test the occurence of islands Sme21T, Sme19T, and Sme80S previously described in the model strain Rm1021. This analysis was conducted using PCR methodology involving specific primers. It was demonstrated that, in the examined geographically distinct populations of S. meliloti from the Northern Caucasus (NCG) and the Aral Sea region (PAG), the strains containing genomic islands were observed with similar frequency (0.55 and 0.57, respectively). Island Sme80S, denoted as an island of “environmental adaptivity,” was identified predominantly (frequency of 0.38) in genomes of strains which exhibited a lower level of salt tolerance and was isolated in PAG, a modern center of introgressive hybridization of alfalfa subjected to salinity. Island Sme21T designated as “ancestral” was observed in genomes of strains isolated in NCG, the primary center of host-plant biodiversity, 10-fold more often than in strains from PAG. An island Sme19T, which predominantly carries genes encoding transposases, was observed in genomes of strains in both populations with average frequency of 0.10. The analysis of linkage disequilibrium (LD) based on the assessment of probability for detection of different islands combinations in genomes revealed an independent inheritance of islands in salt-sensitive strains of various geographic origin. In contrast, the absence of this trend was noted in the majority of the examined combinations of salt-tolerant strains. It was concluded that the structure of chromosome in PAG strains which predominantly possessed a salt-sensitive phenotype was subjected to active recombinant processes, which could predetermine the intensity of microevolutionary processes in bacterial populations and facilitate an adaptation of bacteria in adverse environmental effect. © 2016, Pleiades Publishing, Inc.

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