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Arbuzova E.N.,All Russia Center for Plant Quarantine | Kulinich O.A.,RAS Severtsov Institute of Ecology | Mazurin E.S.,All Russia Research Institute of Agricultural Biotechnology | Ryss A.Y.,Russian Academy of Sciences | And 2 more authors.
Biology Bulletin

Surveys of forests and stockpiled timber of pine, spruce, larch, and silver fir in 14 administrative subjects of the Russian Federation revealed widespread occurrence of the coniferous wood parasitic nematode Bursaphelenchus mucronatus. Twenty species of bacteria belonging to 13 genera have been detected in 25 B. mucronatus isolates, and their identity has been determined by direct sequencing of the 16S RNA gene. The most frequently occurring were bacteria from the genera Pseudomonas, Stenotrophomonas, Pantoea, Bacillus, Burkholderia, and Serratia. Prevalence of Pseudomonas brenneri and P. fluorescence, which were also found in the nematode dauer larva (LIV) isolated from the fir sawyer beetle Monochamus urussovi, have also been assessed. Two nematode B. xylophilus isolates from Portugal and one isolate from the United States have been examined, and 10 symbiotic bacteria species have been isolated, including Agrobacterium tumefacience, P. fluorescens, P. brenneri, Rahnella aquatilis, Stenotrophomonas maltophilia, S. rhizophila, and Yersinia mollaretii. © 2016, Pleiades Publishing, Inc. Source

Sochivko D.G.,JSC Syntol | Fedorov A.A.,Russian Academy of Sciences | Varlamov D.A.,All Russia Research Institute of Agricultural Biotechnology | Kurochkin V.E.,Russian Academy of Sciences | Petrov R.V.,Federal Biomedical Agency
Doklady Biochemistry and Biophysics

The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. The basic principles of PCR mathematical analysis are presented. Approximation of PCR kinetic curves and PCR efficiency curves by various functions is described. Several PCR models based on chemical kinetics equations are suggested. Decision criteria for an optimal function to describe PCR efficiency are proposed. © 2016, Pleiades Publishing, Ltd. Source

Korneeva I.V.,All Russia Research Institute of Agricultural Biotechnology | Varlamova N.V.,All Russia Research Institute of Agricultural Biotechnology | Pushin A.S.,RAS Institute of Chemistry | Firsov A.P.,RAS Institute of Chemistry | And 4 more authors.
Acta Horticulturae

Transgenic tomato plants (Solanum lycopersicum L. syn. Lycopersicon esculentum Mill. 'Yamal') expressed PR-5 protein genes (thau II, kiwi TLP) were obtained via Agrobacterium-mediated transformation. The presence of the foreign genes was detected by PCR; the accumulation of the PR-5 proteins in the leaves and fruits of transgenic plants was confirmed by western blot analyses. Seeds of T1 and T2 generation from transgenic plants with high expression of foreign proteins were obtained in the greenhouse. The biological effect of the foreign PR-5 proteins was verified by greenhouse testing where T2 transgenic tomato plants demonstrated essentially enhanced disease resistance against Phytophthora infestans and Xanthomonas vesicatoria. Homozygous T3 generation seeds from transgenic plants with high resistance were collected. As control of most diseases is extremely difficult, the feasibility of using PR-5 protein genes for genetic engineering plants with improved resistance to various phytopathogens will be discussed. Source

Miroshnichenko D.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Chernobrovkina M.,All Russia Research Institute of Agricultural Biotechnology | Dolgov S.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry
Plant Cell, Tissue and Organ Culture

The efficiency of immature embryo-derived in vitro culture of G genome wheats is significantly influenced by various auxins and sugars which are used for induction of embryogenic response, and by regeneration media composition for promotion of plant development from subcultured embryogenic calli. The embryogenic calli of Triticum timopheevii has demonstrated the highest regeneration ability when the initial explants were cultured on the media supplemented with 4 mg l−1 of Picloram (29.0 %), 4 mg l−1 of Dicamba (28.7 %) or 3 mg l−1 of 2,4-D (29.1 %). The media supplemented with 5–6 mg l−1 of Picloram were considered to be the most effective for promotion of embryogenic/regenerable callus production in Triticumkiharae cultures (73.7–75.0 %). Both T. timopheevii and T. kiharae embryogenic structures were characterized by the formation of green and albino plantlets. Generally the medium that was initially supplemented with Picloram promoted the formation of lower albino plants fraction rather than 2,4-D and Dicamba. As it was measured by the total green plant production per initial explant, the overall efficiency has been reduced when sucrose was substituted by glucose or maltose. The regeneration medium supplemented with 0.25 mg l−1 TDZ significantly enhanced the regeneration capacity of embryogenic callus in T. kiharae. In culture of T. timopheevii the difference between the medium lack of growth regulators and the medium supplemented with TDZ was not prominent, though both of the media have demonstrated the greater efficacy as compared to those supplemented with BA and Zeatin. © 2016 Springer Science+Business Media Dordrecht Source

Komakhin R.A.,All Russia Research Institute of Agricultural Biotechnology | Vysotskii D.A.,All Russia Research Institute of Agricultural Biotechnology | Shukurov R.R.,IBC Generium LLC | Voblikova V.D.,All Russia Research Institute of Agricultural Biotechnology | And 4 more authors.
BMC Biotechnology

Background: In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the β-actin gene. This high level of the gene expression has attracted our attention as an opportunity for the identification of novel strong promoters of plant origin, which could find its application in plant biotechnology. Therefore, in the present study we focused on the nucleotide sequence identification and the functional characteristics of the pro-SmAMP2 promoter in transgenic plants. Results: In chickweed (Stellaria media), a 2120 bp promoter region of the pro-SmAMP2 gene encoding antifungal peptides was sequenced. Six 5'-deletion variants -2120, -1504, -1149, -822, -455, and -290 bp of pro-SmAMP2 gene promoter were fused with the coding region of the reporter gene gusA in the plant expression vector pCambia1381Z. Independent transgenic plants of tobacco Nicotiana tabacum were obtained with each genetic structure. GUS protein activity assay in extracts from transgenic plants showed that all deletion variants of the promoter, except -290 bp, expressed the gusA gene. In most transgenic plants, the GUS activity level was comparable or higher than in plants with the viral promoter CaMV 35S. GUS activity remains high in progenies and its level correlates positively with the amount of gusA gene mRNA in T3 homozygous plants. The activity of the pro-SmAMP2 promoter was detected in all organs of the transgenic plants studied, during meiosis and in pollen as well. Conclusion: Our results show that the pro-SmAMP2 promoter can be used for target genes expression control in transgenic plants. © 2016 Komakhin et al. Source

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