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Mizuike R.,Osaka University | Sasaki T.,Osaka University | Baba K.,Baba Pediatric Clinic | Iwamoto H.,Tanaka Kikinzoku Kogyo Co. | And 10 more authors.
Clinical and Vaccine Immunology | Year: 2011

Since its emergence in April 2009, pandemic influenza A virus H1N1 (H1N1 pdm), a new type of influenza A virus with a triple-reassortant genome, has spread throughout the world. Initial attempts to diagnose the infection in patients using immunochromatography (IC) relied on test kits developed for seasonal influenza A and B viruses, many of which proved significantly less sensitive to H1N1 pdm. Here, we prepared monoclonal antibodies that react with H1N1 pdm but not seasonal influenza A (H1N1 and H3N2) or B viruses. Using two of these antibodies, one recognizing viral hemagglutinin (HA) and the other recognizing nucleoprotein (NP), we developed kits for the specific detection of H1N1 pdm and tested them using clinical specimens of nasal wash fluid or nasopharyngeal fluid from patients with influenza-like illnesses. The specificities of both IC test kits were very high (93% for the HA kit, 100% for the NP kit). The test sensitivities for detection of H1N1 pdm were 85.5% with the anti-NP antibody, 49.4% with the anti-HA antibody, and 79.5% with a commercially available influenza A virus detection assay. Use of the anti-NP antibody could allow the rapid and accurate diagnosis of H1N1 pdm infections. Copyright © 2011, American Society for Microbiology. All Rights Reserved. Source


Dohi T.,Juntendo University | Miyauchi K.,Juntendo University | Ohkawa R.,University of Tokyo | Nakamura K.,University of Tokyo | And 14 more authors.
Atherosclerosis | Year: 2013

Background: Lysophosphatidic acid (LPA) is a platelet activator and highly thrombogenic lipid constituent of atherosclerotic plaque. However, whether or not LPA locally released from culprit lesions is associated with acute coronary syndrome (ACS) remains unclear. Methods: We studied 52 patients with ACS who were treated by emergency percutaneous coronary intervention and thrombectomy. Levels of LPA and other established biomarkers were enzymatically assayed in samples of culprit coronary arterial and systemic peripheral arterial blood. Levels of LPA and lysophosphatidylcholine (LPC) were measured in plasma, and those of autotaxin, soluble CD40 ligand (sCD40L), hs-CRP and Lp-PLA2 were measured in serum. Results: Median LPA levels were significantly higher in coronary (CB) than in peripheral (PB) arterial blood (. p=0.009). Levels of sCD40L were higher in CB than in PB, but the difference did not reach statistical significance (. p=0.177). In contrast, autotaxin and Lp-PLA2 levels were significantly higher in PB than in CB (. p=0.005 and p=0.038, respectively). Levels of LPC and hs-CRP were also higher in PB than in CB (. p=0.129 and p=0.121, respectively). Levels of LPA in both CB and PB were positively and significantly associated with those of LPC (. r=0.632, p<0.01 and r=0.465, p<0.001). Conclusions: Culprit coronary arteries of ACS contained significantly more LPA than the systemic arterial circulation. Higher LPA concentrations might be associated with the pathophysiology of ACS. © 2013 Elsevier Ireland Ltd. Source


Iizuka Y.,University of Tsukuba | Ueda S.,Asahi Kasei Corporation | Hanada T.,Wako Pure Chemical Industries Ltd | Tani W.,Reference Material Institute for Clinical Chemistry Standards | And 7 more authors.
Clinical Chemistry and Laboratory Medicine | Year: 2012

Background: There is a need for a pancreatic lipase (LIP) reference assay to provide an accurate base to which routine methods can be traceable. Methods: This study developed a novel LIP assay method in which 1,2-dioleoylglycerol (DODG) is the substrate and LIP activity is measured in a coupled enzymatic reaction from the increase in absorbance at 340 nm with production of NADPH. Results: With this method, LIP activity was linear up to 440 U/L (8-times expected upper limit of physiological concentration). When assayed manually, the between-laboratory variation for six samples surveyed at five laboratories was 3.8026.4 (CV) for samples containing about 20290 U/L LIP activity; when assayed using an automated analyzer, the range was 1.864.86 (four laboratories). Interference by >5 mmol/L glycerol and low specificity with post-heparin samples were noted, but in practice these are avoidable. Precision analyzed by automated assay of 49 samples twice in random order produced a covariance of 2.27 U/L, which is comparable to routine methods, and good correlations were obtained with five routine methods. Conclusions: Although further studies are required, the DODG method may be likely applicable as one candidate reference method. © 2012 by Walter de Gruyter Berlin Boston. Source


Dohi T.,Juntendo University | Miyauchi K.,Juntendo University | Ohkawa R.,University of Tokyo | Nakamura K.,University of Tokyo | And 12 more authors.
Clinica Chimica Acta | Year: 2012

Background: The platelet activator lysophosphatidic acid (LPA) has recently been identified as an ingredient in oxidized LDL and it has been isolated from atherosclerotic plaques. The lysophospholipase D activity of autotaxin produces LPA extracellularly from lysophosphatidylcholine (LPC). The present study determines whether circulating LPA is associated with acute coronary syndrome (ACS). Methods: We enrolled 141 consecutive patients (age, 62.6 ± 3.8 y; male, 69.2%) with ACS (n = 38), stable angina pectoris (SAP; n = 72) or angiographically normal coronary arteries (NCA; n = 31). The relationships between LPA and other established biomarkers were examined. Concentrations of plasma LPA were determined using an enzymatic assay. Results: Concentrations of LPA significantly correlated with LPC (r = 0.549), autotaxin (r = 0.370) and LDL-C (r = 0.307) (all p < 0.01). Lysophosphatidic acid concentrations were significantly higher in patients with ACS than with SAP and NCA (p < 0.01), but did not significantly differ between patients with SAP and NCA. Multivariate logistic regression analyses revealed that the highest LPA tertile was independently associated with ACS (odds ratio 1.99, 95% CI: 1.18-3.39, p = 0.02). Conclusions: The present study demonstrated that increased circulating plasma LPA concentrations are significantly associated with ACS. © 2011 Elsevier B.V. Source


Patent
Alfresa Pharma Corporation | Date: 2010-10-20

Disclosed is a flat cable formed by braiding ultra-high molecular weight polyethylene fibers, in which degree of the fibers close contact in a condition where no tensile force is applied is elevated compared with conventional ones. The cable is a flat cable formed by braiding ultra-high molecular weight polyethylene fibers, the area of whose cross section under a load of no tensile stress is not more than 110% of the cross section area under a load of a tensile stress of 22.7 N/mm

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