Ahmadlou S.,Tehran University of Medical Sciences |
Akhiani M.,Alborz Hospital |
Salimzadeh A.,Sina |
Keramatipour M.,Tehran University of Medical Sciences
Iranian Journal of Allergy, Asthma and Immunology | Year: 2014
The association of rs10818488 SNP located in TRAF1/C5 region with Rheumatoid Arthritis (RA), has been picked up by genome-wide association studies. Independent studies in different populations revealed inconsistent results. The aim of this study was to investigate the possible association of this SNP with RA in Iranian population. A total of 362 cases and 422 healthy controls were recruited in this study. Genomic DNA was extracted from whole blood and the genotyping was performed by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). A set of genotypes was confirmed by sequencing. Genotype and allele frequencies were compared between the case and control groups. Analysis indicated a higher frequency of A allele in cases, although the difference was not statistically significant (Chi-square=2.8, p=0.09). Comparison of genotype frequencies, revealed higher frequencies of AA and AG genotypes in case group but statistically the difference was not significant (Chi-square=2.72, p=0.25). These findings suggest that the rs10818488 in TRAF1/C5 region is not associated with rheumatoid arthritis in Iranian population. Copyright© Winter 2014, Iran J Allergy Asthma Immunol. All rights reserved.
Ebadi P.,Islamic Azad University |
Daneshmandi S.,Tarbiat Modares University |
Ghasemi A.,Alborz Hospital |
Karimi M.H.,Shiraz University of Medical Sciences
Molecular Biology Reports | Year: 2013
Gallstone is a common biliary disorder with several risk factors. Immune responses and inflammatory cytokines are important in this disease; as a result, some cytokines can be detected in bile fluid. In this research, cytokine gene polymorphisms were studied, and their effects on gallstone formation were evaluated. On 158 gallstone patients and 254 normal subjects, by PCR-RFLP method, IL-4-C590T polymorphism and by ARMS-PCR method, IFN-γ T+874A, TNF-α-A308G, IL-6 G-174C and TGF-β T+869C variants were studied. Pathologic evaluations were done on surgical specimens. There were no significant differences in distribution of evaluated polymorphisms between patient group and normal control group (P>0.05), except TGF-β +869T allele (P = 0.04, OR = 1.23, 95 % CI = 1-1.79) which was higher in patients with gallstone. Although the pro-inflammatory cytokines such as TNF-a and IL-6 may promote gallstone formation, in this study no significant correlation between TNF-a and IL-6 polymorphisms and gallstone formation was seen. It is taught that TGF-β may affect gallbladder cells to promote gallstone formation and higher producer TGF-β +869T allele can be a risk factor of gallstone disease, so further studies would be more elucidative. © 2013 Springer Science+Business Media Dordrecht.
Mohammadi S.Z.,Islamic Azad University at Karaj |
Shahparian M.,Alborz Hospital |
Fahidy F.,Shahriar Hospital |
Fallah E.,Shahriar Hospital
ARYA Atherosclerosis | Year: 2012
BACKGROUND: Erectile dysfunction (ED) is the inability to achieve or maintain the adequate erection for intercourse. Heart failure is a major risk factor for erectile dysfunction. The aim of this study was to investigate the prevalence and factors associated with erectile dysfunction in systolic heart failure. METHODS: In a cross-sectional study 100 male patients with systolic heart failure were selected using convenience sampling method. IIEF-5 questionnaire (the International Index of Erectile Function, 5-item version), MLHFQ (Minnesota Living with Heart Failure Questionnaire) and CES-D (Centre for Epidemiologic Studies Depression Scale) were used to obtain data. RESULTS: Mean score of erectile dysfunction was 14.02 ± 6.26 and 80% of heart failure patient had erectile dysfunction. Erectile dysfunction was significantly associated with age (P < 0.001), education (P = 0.019), occupation (P = 0.002), hemoglobin level (P = 0.003), left ventricular ejection fraction (P = 0.030), cholesterol level (P = 0.001), renal dysfunction (P = 0.009), use of digoxin (P = 0.014), angiotensin converting enzyme inhibitors (P < 0.001), beta blocker (P = 0.001), diuretics (P = 0.035), depression (P < 0.001) and quality of life (P < 0.001). CONCLUSION: Erectile dysfunction (ED) was common in systolic heart failure and was associated with age, medical conditions, co morbidities, drugs for treatment and psychological disorders. In heart failure patients erectile dysfunction had negative impact on quality of life.
Hamayeli Mehrabani H.,Faculty and Institute Nutrition science and Food Technology |
Tahbaz F.,Faculty and Institute Nutrition science and Food Technology |
Salehpour S.,Fertility Research Center |
Hedayati M.,Shahid Beheshti University of Medical Sciences |
And 2 more authors.
Iranian Journal of Endocrinology and Metabolism | Year: 2010
Introduction: The appropriate combination and composition of hypocaloric diets for obese women with polycystic ovary syndrome (PCOS) is still unclear. The aim of this study was to investigate the effects of a high protein-low glycaemic load diet on reproductive hormones in obese women with PCOS. Materials and Methods: In this single blind clinical trial, sixty overweight and obese women with PCOS, not using metformin, were randomly assigned in one of two groups, the modified with a 30% protein-low glycaemic load diet, and the conventional with 15% protein. Reproductive hormones, glucose and insulin were measured for 49 subjects at baseline and after 12 weeks. Results: Weight loss showed no significant difference in the two groups. Changes in means for testosterone in the modified and conventional groups (0 to 12 weeks), were 1.78±1.52 to 1.31±1.27 and 1.51±0.63 to 1.15±0.57 ng/ml, respectively (p<0.001). FSH, LH, estradiol, progesterone and prolactin did not change. Insulin and HOMA reduction was significant for modified diet (p<0.001). Conclusion: Weight loss had a significant effect on reduction of androgens. The main effect of modified diet with high protein and low glycaemic load was observed on insulin and HOMA, a finding which should taken into consideration for women with PCOS.
Ahmadloo S.,Tehran University of Medical Sciences |
Taghizadeh M.,Tehran University of Medical Sciences |
Akhiani M.,Alborz Hospital |
Salimzadeh A.,Tehran University of Medical Sciences |
Keramatipour M.,Tehran University of Medical Sciences
Iranian Journal of Allergy, Asthma and Immunology | Year: 2015
The rs2476601 (R620W, C1858T) polymorphism in PTPN22 gene has been repeatedly reported to be associated with rheumatoid arthritis (RA). The rs 2476601 is widely suggested for predictive testing and risk assessment for RA. The aim of this study was to test the possible association of this SNP with RA in Iranian population. A total of 872 samples (405 confirmed RA patients and 467 healthy controls) were recruited in this study. Genomic DNA was extracted from whole blood and the genotyping was performed by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). Genotyping for a set of samples were re-confirmed by two other rounds of genotyping, using another PCR-RFLP experiment with different enzyme and DNA sequencing. All 872 samples were genotyped as homozygous CC in first round of genotyping. Genotyping was repeated for 30% of samples by another restriction enzyme and for 10% of samples by sequencing. Again all samples showed homozygous CC genotype. This study suggests that the rs2476601 polymorphism of PTPN22 gene is mono-morphic in Iranian population, containing only C allele. Considering that previous studies in other populations reported the T allele as the risk allele at this locus, the present study concluded that rs2476601 play no role in susceptibility to RA and other autoimmune diseases in Iranian population. This finding has significant future clinical implications in determining the strategy for risk assessment and predictive testing for such diseases in Iranian population. Copyright© Summer 2015, Iran J Allergy Asthma Immunol. All rights reserved.