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Seday U.,Alata Horticultural Research Station | Uzun A.,Erciyes University | Yilmaz C.,Eskiehir Osmangazi University | Eti S.,Cukurova University
Bulgarian Journal of Agricultural Science | Year: 2013

Pomegranate is well-adapted fruit species to arid and semi-arid conditions. This species have male (A type - unfertile) and hermaphrodite flowers (B type - fertile) on the same plant. Because of several problems, appear for fertilization and fruit set, researches on pollen quality and fruit set may be essential. In the present study pollen viability, germination, pollen quantity of A and B type flowers of seven pomegaranate cultivars were investigated. Pollen viability assessment was carried out using 1% 2, 3, 5 triphenyl tetrazolium chloride (TTC) test. Pollen germination capability was investigated agar in petri method by using four different media. Pollen production per anther and per flower was assayed by Hemacytometric method. In addition, fruit set of these cultivars under self-pollination condition was determined. The highest pollen viability level obtained from 'Mayhos VIII' and 'Hicaz' cultivars. Pollen germination was assayed using 1% agar and different sucrose concentrations (5, 10, 15 and 20%). In general, 15% and 20% sucrose concentrations determined as the best germination medium. Anther number per flower ranged between 275 and 410 according to the cultivars. 'Katirbasi' had the highest pollen number per flower whereas 'Cevlik' had the lowest. Self-pollination of "Hicaz" with B type flowers showed the best fruit set. Besides, "Eksi Goknar" did not provide any fruit on self-pollination treatments. For that reason, this cultivar can determine as self-incompatible. This study showed that, some cultivars need pollinators to set fruit and this should be considered for new plantations. Source

Oluk A.C.,Alata Horticultural Research Station | Oluk A.C.,Cukurova University | Guven M.,Cukurova University | Hayaloglu A.A.,Inonu University
International Journal of Food Science and Technology | Year: 2014

The objective of the study was to determine the effects of exopolysaccharide (EPS)-producing or non-EPS-producing starters on proteolysis, physical and microstructural characteristics of full-fat or low-fat Tulum cheeses during ripening. For this purpose, Tulum cheese was manufactured using full- or low-fat milk with EPS-producing and non-EPS-producing starter cultures. Chemical composition, proteolysis, texture profiles and microstructure of the cheeses were studied during 90 days of ripening. Urea-PAGE of water-insoluble and RP-HPLC peptide profiles of water-soluble fractions of the cheeses showed that the use of starters resulted in different degradation patterns in all cheeses during ripening. Although β-casein exhibited similar degradation patterns in all cheeses, small differences are present in αs1-casein degradation during ripening. Reducing fat in Tulum cheese changed the RP-HPLC peptide profile of the cheeses. The use of EPS-producing cultures improved the textural characteristics and changed the microstructure and proteolysis of low-fat Tulum cheese. © 2013 Institute of Food Science and Technology. Source

Akyol E.,Nigde University | Unalan A.,Nigde University | Yeninar H.,Kahramanmaras Sutcu Imam University | Ozkok D.,Erciyes University | Ozturk C.,Alata Horticultural Research Station
Italian Journal of Animal Science | Year: 2014

This study was carried out to determine the performances of Mugla and Nigde ecotypes (A. m. anatoliaca), Caucasian (A. m. caucasica) and Carniolan (A. m. carnica) honeybee genotypes in the region of central Anatolia conditions. A total of 40 colonies (each genotype group consisted of 10 colonies) were used in the study. All queens were reared at the same time and in the same apiary and were instrumentally inseminated. The average numbers of combs covered with bees were found to be 11.24±0.59, 9.51±0.42, 8.11±0.31 and 12.38±0.72 per colony respectively; the average brood areas were found to be 2825.0±240.3, 2160.6±176.8, 1701.9±129.7 and 2883.0±104.4 cm2 per colony respectively; and the average honey yields were found to be 28.60±0.88, 15.40±0.69, 23.40±0.54 and 31.60±1.12 kg per colony in Mugla ecotype, Nigde ecotype, Caucasian and Carniolan genotypes respectively. The mean differences among the genotypes for number of combs with bees, brood area and honey yield were significant (P<0.01). These results showed that the Carniolan genotype had the best performance and Nigde ecotype had the lowest performance in temperate climate conditions. Therefore, productivity of the Nigde ecotype can be increased by a crossbreeding programme with Carniolan and Mugla genotypes. © E. Akyol et al., 2014. Source

Gurcan K.,Erciyes University | Say A.,Erciyes University | Yetisir H.,Erciyes University | Denli N.,Alata Horticultural Research Station
Genetic Resources and Crop Evolution | Year: 2015

Bottle gourd [Lagenaria siceraria (Molina) Standl.] is one of the oldest cultivated plants, native to Africa and it distributed to over the World. Its dispersal route from Africa to America and Asia is still subject of studies. Similarly, when and how bottle gourd entered into Turkey is not known. In previous studies, bottle gourd germplasm collecting activities were undertaken in the country and the germplasm were analyzed morphologically. Here we report molecular analysis of 60 Turkish bottle gourds along with 31 diverse exotic accessions. Eighth Simple Sequence Repeats and two chloroplast loci of 91 accessions were analyzed. Capillary electrophoresis was used for DNA size fragman separation. The number of alleles per locus ranged from 2 to 13, with a mean of 7. Mean values for expected heterozygosity, observed heterozgosity, and polymorphism information averaged 0.5, 0.13 and 0.50, respectively, thereby sugguesting very low (0.13) genetic diversity in a very diverse population. An unweighted pair group method with arithmetic mean dendrogram was constructed, Indian accessions clearly separated from the rest. Among the remaining samples, neither Turkish landraces grouped together based on their georgraphic origin, nor clear speration occurred according to origin continent. Interestingly, this co-dominant marker analysis shows close molecular allelic profile among bottle gourds originated from far different countries. © 2015, Springer Science+Business Media Dordrecht. Source

Uzun A.,Erciyes University | Seday U.,Alata Horticultural Research Station | Canihos E.,Plant Protection Research Institute | Gulsen O.,Erciyes University
Experimental Agriculture | Year: 2012

Citrus trees are often exposed to severe infectious diseases. Mal secco caused by Phoma tracheiphila (Petri) Kantschaveli and Gikashvili is one of the most destructive fungal diseases of lemons (Citrus limon Burm. F.). In the present study, antioxidant enzyme activity in different mal secco-resistant and susceptible citrus rootstocks including Cleopatra mandarin (C. reshni Tan.), sour orange (C. aurantium L.), rough lemon (C. jambhiri Lush.), Volkameriana (C. volkameriana Tan. and Pasq.), Carrizo citrange (Poncirus trifoliata L. Raf. X C. sinensis L. Osbeck) and trifoliate orange (P. trifoliata) was investigated. Possible differences in constitutive levels of these antioxidant enzymes and correlations between enzyme levels and mal secco caused by P. tracheiphila were examined. Among the rootstocks, Cleopatra mandarin was found to be resistant to mal secco, whereas rough lemon, sour orange and trifoliate orange were highly susceptible. Total peroxidase (TPX; EC: activity increased in all infected rootstocks. Ascorbate peroxidase (APX; EC: activity increased in most of the rootstocks and no correlation was found between catalase (CAT; EC: activity and mal secco resistance. This study indicates that overall TPX activity is upregulated and APX activity is up-and down-regulated depending on the type of rootstock in response to P. tracheiphila infection. © 2012 Cambridge University Press. Source

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