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Pinar H.,Alata Horticultural Research Station | Uzun A.,Erciyes University | Unlu M.,Alata Horticultural Research Station | Bircan M.,Alata Horticultural Research Station | And 3 more authors.
Bulgarian Journal of Agricultural Science | Year: 2015

This study was conducted to estimate genetic relationships and natural somatic mutations among selected banana genotypes from different region of Turkey via SRAP molecular markers. Ninety-six banana genotypes including 39 ‘Dwarf Cavendish’, 18 ‘Grande Nain’, 28 ‘Azman’ and 11 other bananas were evaluated. A total of 102 bands were obtained from 19 SRAP primer combinations and 86 (85.5%) of them were polymorphic. Number of bands per primer combination was 5.3 whereas polymorphic bands per primer combinations were 4.5. Genetic similarity of 96 accessions varied between 0.63-1.00. It was determined genetic variation among the accessions which may allow new selections for breeding programs. Considerable variation was also found among genotypes within the same banana cultivar and it was very important for banana breeding and germplasm evaluation. It can be concluded that SRAP markers could readily be used to estimate genetic relationhips among banana genotypes and natural somatic mutations. © 2015, National Centre for Agrarian Sciences. All Rights reserved.


Kafa G.,Alata Horticultural Research Station | Seday U.,Alata Horticultural Research Station | Uysal O.,Alata Horticultural Research Station | Polatoz S.,Alata Horticultural Research Station
Acta Horticulturae | Year: 2015

Three variegated 'Clementine' mutants were selected at the Alata Horticultural Research Station of Mersin among the M1 V3 'Clementine' plants obtained from budwood Gamma ray irradiation at 30 gray. Young fruit rind color is attractively variegated but fruit rind color variegation fades with maturity and mature fruit rind color could easily masquerade as an ordinary 'Clementine'. The young fruit rind is striped green and orange, but mature fruit rind has ridges. Seed number per fruit is reduced compared to the 'Clementine' control plants and vary between 7.5 and 13.07. Fruit weight are 72.73, 69.3 and 80.94 g, respectively, for variegated Clementine No.1, 2 and 3 and 110.10 g for control 'Clementine' plants. Other pomological characteristics are the same for variegated and control plants.


Polatoz S.,Alata Horticultural Research Station | Tuzcu O.,Cukurova University
Acta Horticulturae | Year: 2015

Due to the various climatic and soil characteristics existent in Turkey many fruits species can be grown economically. Citrus are grown in the Mediterranean region especially at the southern part of Toros mountains for many centruies. In the recent years citrus growth has increased year by year worldwide. In this study, second level selection lines of nucellar clones of important 20 local and foreign orange cultivars were used. These lines were planted in the research plot from 1975 to 1980. These genotypes were examined for 22 pomological and 4 plant characteristics. Fruits were harvested at optimal maturation stage and yield/tree rates were calculated individually. The twenty nucellar orange cultivars, named 'Hamlin (14-17)', 'Hamlin (14-15)', 'Magnum Bonum (15-10)', 'Trovita (A-35)', 'Parson Brown (18-17)', 'Portakal (P-24) (20-13)', 'Portakal (P-28) (21-10)', 'Shamouti WN', 'Yafa (14-4)', 'Yafa (A-21)', 'Balady (15-8)', 'Madame Vinous (A-36)', 'Dörtyol Yerli (17-3)', 'Pineapple (18-10)', 'Barile (16-11)', 'Kozan Yerli (17-9)', 'Biondo (20-8)', 'Biondo Ricco (19-3)', 'Paperrind (18-5)' and 'Valencia (16-16)' were evaluated in terms of their yield and fruit quality characteristics. The cultivars 'Hamlin (14-15)', 'Balady (15-8)', 'Madame Vinous (A-36)', 'Trovita (A-35)' and 'Valencia (16-16)' were found very promising.


Kafa G.,Alata Horticultural Research Station | Seday U.,Alata Horticultural Research Station | Uysal O.,Alata Horticultural Research Station | Polatoz S.,Alata Horticultural Research Station
Acta Horticulturae | Year: 2015

In 2004, 'Clementine', 'Nova' and 'Robinson' budwood was Gamma irradiated at 30 and 50 gray doses. Irradiated buds were grafted on sour orange to produce M1V1 (first stage of mutation and vegetation) plants. Thus, M1V2 plants were produced from buds from M1V1 plants grafted on sour orange. In the same way, M1V3 plants have been obtained from M1V2 buds grafted on sour orange. In 2007, a total of 675 M1V3 plants were planted in the field with 5?2 m spacing. Ten non irradiated control plants were also planted in the same field. For two years, seed number of 135 'Clementine', 110 'Nova', 165 'Robinson' potential mutants and control plants were determined. Fruits of each plant were harvested at maturity (November) in 2010-2011 and average seed number of all plants was determined. As a result, budwood irradiation decreased seed number in resulting mutant plants, especially for 'Nova' and 'Robinson' mandarins.


Uzun A.,Erciyes University | Gulsen O.,Erciyes University | Kafa G.,Alata Horticultural Research Station | Seday U.,Alata Horticultural Research Station
Acta Horticulturae | Year: 2015

Some citrus species and cultivars with attractive appearance of fruits and leaves have been used for ornamental purposes. Some of them have small fruits and plant size and mixed color of leaves or fruits. Here, we report a new lemon genotype with potential use for ornamental purposes. This genotype was obtained exposing 'Kutdiken' lemon cultivar budwood to gamma irradiation. This cultivar is widely grown in Turkey because of its high yield and good quality fruits. Fruits of the new genotype have attractive appearance with vertical brown lines and brown point heaps. Differences of fruit rind of this genotype have originated from mutation(s) caused by gamma irradiation.


Seday U.,Alata Horticultural Research Station | Gulsen O.,Erciyes University | Uzun A.,Alata Horticultural Research Station | Toprak G.,Erciyes University
Journal of Animal and Plant Sciences | Year: 2014

Citrus is the most produced fruit crop and often constrained by salinity due to their geographic distribution. We investigated morphological and antioxidave enzyme responses of six different citrus rootstocks under various NaCl concentrations, including Cleopatra mandarin (Citrus reshni Tan.), sour orange (C. aurantium L.), rough lemon (C. jambhiri Lush.), Volkamer lemon (C. volkameriana Tan & Pasq.), Carrizo citrange (Poncirus trifoliata L. Raf. X C. sinensis L. Osbeck) and trifoliata orange (P. trifoliata Raf.). Morphological and antioxidative enzyme responses of plants grown in hydroponic media containing 0, 45, 90 and 135 mM of NaCl were assessed at day 10, 20, 30, and 40. The highest shoot growth was observed in Cleopatra mandarin whereas trifoliata orange had the lowest shoot growth. Carrizo citrange showed the lowest visible leaf symptoms among the rootstocks and rough lemon produced the highest level of root biomass. A rootstock x NaCl dose x exposure time interaction was detected for enzymatic responses. In general, ascorbate peroxidase (APX; EC: 1.11.1.11) and guaiacol peroxidase (GP; EC: 1.11.1.7) activity in the rootstocks increased with the elevated salt level. Superoxide dismutase (SOD; EC: 1.15.1.1) activity was lowered in leaves of the rootstocks subjected to higher NaCl concentrations. In conclusion, this study indicated complexity of salt tolerance mechanism probably due to complex genetic make-up of the citrus rootstocks used and importance of APX and GP in salt tolerance of Citrus.


Oluk A.C.,Alata Horticultural Research Station | Oluk A.C.,Cukurova University | Guven M.,Cukurova University | Hayaloglu A.A.,Inonu University
International Journal of Food Science and Technology | Year: 2014

The objective of the study was to determine the effects of exopolysaccharide (EPS)-producing or non-EPS-producing starters on proteolysis, physical and microstructural characteristics of full-fat or low-fat Tulum cheeses during ripening. For this purpose, Tulum cheese was manufactured using full- or low-fat milk with EPS-producing and non-EPS-producing starter cultures. Chemical composition, proteolysis, texture profiles and microstructure of the cheeses were studied during 90 days of ripening. Urea-PAGE of water-insoluble and RP-HPLC peptide profiles of water-soluble fractions of the cheeses showed that the use of starters resulted in different degradation patterns in all cheeses during ripening. Although β-casein exhibited similar degradation patterns in all cheeses, small differences are present in αs1-casein degradation during ripening. Reducing fat in Tulum cheese changed the RP-HPLC peptide profile of the cheeses. The use of EPS-producing cultures improved the textural characteristics and changed the microstructure and proteolysis of low-fat Tulum cheese. © 2013 Institute of Food Science and Technology.


Gurcan K.,Erciyes University | Say A.,Erciyes University | Yetisir H.,Erciyes University | Denli N.,Alata Horticultural Research Station
Genetic Resources and Crop Evolution | Year: 2015

Bottle gourd [Lagenaria siceraria (Molina) Standl.] is one of the oldest cultivated plants, native to Africa and it distributed to over the World. Its dispersal route from Africa to America and Asia is still subject of studies. Similarly, when and how bottle gourd entered into Turkey is not known. In previous studies, bottle gourd germplasm collecting activities were undertaken in the country and the germplasm were analyzed morphologically. Here we report molecular analysis of 60 Turkish bottle gourds along with 31 diverse exotic accessions. Eighth Simple Sequence Repeats and two chloroplast loci of 91 accessions were analyzed. Capillary electrophoresis was used for DNA size fragman separation. The number of alleles per locus ranged from 2 to 13, with a mean of 7. Mean values for expected heterozygosity, observed heterozgosity, and polymorphism information averaged 0.5, 0.13 and 0.50, respectively, thereby sugguesting very low (0.13) genetic diversity in a very diverse population. An unweighted pair group method with arithmetic mean dendrogram was constructed, Indian accessions clearly separated from the rest. Among the remaining samples, neither Turkish landraces grouped together based on their georgraphic origin, nor clear speration occurred according to origin continent. Interestingly, this co-dominant marker analysis shows close molecular allelic profile among bottle gourds originated from far different countries. © 2015, Springer Science+Business Media Dordrecht.


Oluk A.C.,Alata Horticultural Research Station | Oluk A.C.,Cukurova University | Guven M.,Cukurova University | Hayaloglu A.A.,Inonu University
International Journal of Dairy Technology | Year: 2014

The objective of this investigation was to compare the composition and changes in the concentration of volatiles in low-fat and full-fat Tulum cheeses during ripening. Tulum cheese was manufactured from low- or full-fat milk using exopolysaccharide (EPS)-producing or non-EPS-producing starter cultures. A total of 82 volatile compounds were identified belonging to the following chemical groups: acids (seven), esters (21), ketones (14), aldehydes (six), alcohols (14) and miscellaneous compounds (20). The relative amounts of acids, alcohols and aldehydes increased in the cheeses made with EPS-producing cultures during 90 days of ripening. Differences were found in the volatile profile of full-fat Tulum cheese compared with the low-fat variant, especially after 90 days of ripening. Exopolysaccharide-producing cultures changed the volatile profile, and the EPS-producing cultures including Streptococcus thermophilus + Lactobacillus delbrueckii subsp. bulgaricus + Lactobacillus helveticus (LF-EPS2) produced cheese with higher levels of methyl ketones and aldehydes than the non-EPS cultures. In the sensory analysis, full-fat Tulum cheeses and the cheese produced with the EPS-producing culture containing Lb. helveticus (LF-EPS2) were preferred by the expert panel. It was concluded that the use of EPS-producing starter cultures in the manufacture of low-fat Tulum cheese had the potential to improve the flavour. © 2014 Society of Dairy Technology.


Uzun A.,Erciyes University | Gulsen O.,Erciyes University | Seday U.,Alata Horticultural Research Station | Yesiloglu T.,Cukurova University | Kacar Y.A.,Cukurova University
Genetic Resources and Crop Evolution | Year: 2014

Many stress-related interactions such as pathogen infection, insect tolerance, salt tolerance, auxin degradation, cell wall lignification, tissue suberization, and plant senescence involve various isoforms of peroxidases. Peroxidase gene polymorphism (POGP) markers have been used to estimate diversity, relationships and population structure among 80 Citrus and their relatives in Aurantioideae by using unweighted pair group method arithmetic average (UPGMA) and bayesian substructuring analysis. Fourteen primers produced a total of 148 fragments and 147 of them were polymorphic. The UPGMA analysis demonstrated that the accessions had a similarity range from 0.27 to 0.98 and were distinguished. The results of this study were mostly consistent with previous reports of different marker systems, but few different findings were also detected. The subtribe Clauseninae (tribe Clauseneae) did not clearly separate from the subtribes of the tribe Citreae. Substructuring analysis indicated that there were six subpopulations among the accessions studied. This study revealed that the POGP markers can be utilized to estimate genetic diversity, relationships and population structure in Citrus and related species in the Aurantioideae subfamily. © 2014, Springer Science+Business Media Dordrecht.

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