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Sezen S.M.,Tarsus Research Institute | Celikel G.,Alata Horticultural Research Institute | Yazar A.,Cukurova University | Tekin S.,Cukurova University | Kapur B.,Cukurova University
Scientific Research and Essays | Year: 2010

Global warming and resulting drought is the most important constraint affecting plant production in the Mediterranean Region. Therefore, effective management of scarce water resources is of paramount importance in this region. This research was conducted to determine the optimal irrigation strategy for drip irrigated fresh market tomato grown in different soilless culture in a glasshouse in the Mediterranean Region of Turkey. Volcanic ash, peat and their mixture were used as growth media. Four different irrigation levels (WL1=75%; WL2=100%; WL3=125% and WL4=150% of Class A Pan evaporation) and two watering frequencies (once and twice daily applications) were evaluated. Highest yield and fruit number were obtained from the ash+peat mixture (1:1) with twice a day watering at WL4 irrigation level. Soluble solids of tomato fruit decreased with increasing available water. The highest irrigation water use efficiency (IWUE) value of 121.4 kg m-3 was obtained from once a day irrigation WL1 irrigation level with peat+ash (1:1). IWUE decreased in all treatments as the amount of irrigation water increased. © 2010 Academic Journals.

Kurt S.,Alata Horticultural Research Institute | Buyukalaca S.,Cukurova University
Bioresource Technology | Year: 2010

Pleurotus ostreatus and Pleurotus sajor-caju were studied for their ability to produce laccase and carboxymethylcellulase (CMCase) enzymes on different agricultural wastes under solid state fermentation. The spawns of P. ostreatus and P. sajor-caju were inoculated on different agricultural wastes including viticulture wastes, wheat straw, paddy straw, sesame straw, sawdust as well as the mixtures of these wastes with wheat bran. The carbon and nitrogen contents of substrates containing bran were the highest. The laccase activities of P. ostreatus and P. sajor-caju reached the highest values on the day 10 of mycelial growth. This enzyme activity was higher on the substrates containing bran which had high nitrogen and low C/N ratio than the other tested substrates with no bran. The CMCase activities of P. sajor-caju and P. ostreatus had two peaks on the 5th day of mycelial growth and after first flash. P. ostreatus and P. sajor-caju grown on substrates containing wheat bran had higher biological efficiencies and total yields as well as higher CMCase and laccase activities. © 2009 Elsevier Ltd. All rights reserved.

Taskin H.,Cukurova University | Buyukalaca S.,Cukurova University | Keles D.,Alata Horticultural Research Institute | Ekbic E.,Adiyaman University
African Journal of Biotechnology | Year: 2011

Five pepper genotypes (A71, A269, A313, A109 and A74) and four different culture media were tested in this study carried out at the University of Çukurova, Turkey. The anthers were cultured at different periods in order to optimize the frequency of embryo production. Moreover, the embryos that were unable to complete their growth in the culture medium studied were placed in a medium containing 0.5 mgL-1 abscisic acid for 10 days. At the end of the study, it was determined that embryo development varied with genotype, anther cultivation period and culture medium. The highest yield of embryos was obtained from A269, one of the genotypes tolerant to low temperature. The anthers cultured from April to May, gave the highest yields of embryos compared to anthers from the other periods. Most of the embryos were obtained from Medium III (MS medium containing 4 mg/L NAA, 1 mg/L BAP, 0.25% activated charcoal, 15 mg/L AgNO3, 30 g/L sucrose) and the Medium IV (modified MS medium containing 0.25% activated charcoal, 15 mg/L AgNO3, 4 mg/L NAA, 0.1mg/L BAP and with 0.5 mg/L ABA). There was no positive effect of abscisic acid on the mature embryos. © 2011 Academic Journals.

Gulsen O.,Erciyes University | Kaymak S.,Egirdir Horticultural Research Institute | Ozongun S.,Egirdir Horticultural Research Institute | Uzun A.,Alata Horticultural Research Institute
Scientia Horticulturae | Year: 2010

Plant peroxidases belong to a multigene family and posses highly conserved domains allowing to design oligonucleotide primers to amplify DNA sequences coding for peroxidases from plants with unsequenced genomes. Peroxidase gene-based polymorphism among Malus species, and correlation between peroxidase markers were deduced in this study. Turkish apple germplasm was evaluated using 14 peroxidase specific primers. Targeted-PCR amplification of genomic DNA from 192 apple genotypes available in the Turkish genetic resources yielded polymorphisms giving a similarity range from 0.56 to 0.98 with a mean of 0.77. Based on the POGP gene polymorphism, two distinct clusters were detected among the apple accessions, suggesting different evolutionary pathway. Correlation estimates as an indication of linkage disequilibrium between POGP markers ranged from -0.22 to 0.90, suggesting that few POGP markers were clustered and the remaining POGP markers evenly distributed throughout the apple genome. These results demonstrate that primers targeting the peroxidase gene family can be used to study genotypic diversity and evolutionary relationships on an intra- and inter-specific basis. © 2010 Elsevier B.V. All rights reserved.

Yildiz M.,Yuzuncu Yil University | Ekbic E.,Adiyaman University | Keles D.,Alata Horticultural Research Institute | Sensoy S.,Yuzuncu Yil University | Abak K.,European University of Lefke
Scientia Horticulturae | Year: 2011

The genetic relationships among 63 melon (Cucumis melo L.) genotypes collected from various regions of Turkey were determined by comparing their molecular ISSR, SRAP, and RAPD markers with those of 19 foreign melon genotypes to investigate the taxonomic relationships and genetic variation of Turkish melon germplasm. Total 162 polymorphic markers (69, 18, and 75 obtained from ISSR, SRAP, and RAPD primers, respectively) were used to define the genetic similarity among the melon genotypes by dendrogram or two and three dimensional scalings. The average similarity (SM coefficient) between any two pairs of accessions examined as estimated by molecular variation was 0.73 ± 0.48. Within-group genetic similarities ranged between 0.46 and 0.96. Related genotypes or genotypes collected from similar regions were partitioned to similar clusters. Southeastern Anatolian genotypes were distinctly apart from group inodorus and group cantalupensis (sweet) genotypes. This reinforced the position of Turkey in the secondary genetic diversity center of melon. The genetic diversity among Turkish genotypes (H= 0.28 and I= 0.42) was only a little less than that of the world accessions (H= 0.30 and I= 0.45). On the other hand, the percentage of polymorphic loci among Turkish melon genotypes (90.7%) was even higher than that of the world accessions (87.6%). © 2011 Elsevier B.V.

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