University of Al Zawiya

University of Technology of Compiègne, France

University of Al Zawiya

University of Technology of Compiègne, France
SEARCH FILTERS
Time filter
Source Type

Kanala K.M.,Jawaharlal Nehru University | Kanala K.M.,Ratnam Institute of Pharmacy | Chandu B.R.,University of Al Zawiya | Hwisa N.T.,University of Al Zawiya | And 2 more authors.
Journal of Pharmacy Research | Year: 2013

Background: The purpose of this investigation was to explore high selective, sensitive, rapid, stable, reproducible extraction method in long run with broader linear range. At the same time, it could be expected that, this method would be efficient in analyzing large numbers of plasma samples obtained for pharmacokinetic, bioavailability or bioequivalence studies. Methods: A simple, sensitive, selective and rapid high-performance liquid chromatography coupled with tandem mass spectrometry was developed and validated for quantification of Acamprosate in human plasma. The chromatography was performed by using waters atlantis HILIC, (2.1 mm × 50 mm, 3.0 μm) column connected with guard column waters atlantis HILIC, (2.1 mm × 10 mm, 3.0 μm). Acamprosate-d12 calcium trihydrate used as an IS. The extraction of drug and internal standard were obtained by solid phase extraction. The linearity was proved with concentration range 1.00-250.00 ng/ml for Acamprosate in human plasma. Results and discussion: The LOQ was demonstrated at 1.00 ng/ml. The within-batch, between-batch precision was found to be 2.21-4.07% and 2.00-3.20%. The within-batch, between-batch accuracy was found to be 96.26-102.00% and 98.27-102.00% for Acamprosate. Drug and IS were eluted within 3.0 min. Conclusion: The developed LC-MS/MS assay for Acamprosate is rapid, simple, sensitive, selective and suitable for routine measurement of sample analysis. The validated method was successfully applied in pharmacokinetic study of human plasma. © 2013, JPR Solutions; Published by Reed Elsevier India Pvt. Ltd.

Loading University of Al Zawiya collaborators
Loading University of Al Zawiya collaborators