PubMed | University of Rome La Sapienza, BLDE University and Al Ameen Medical College
Type: Journal Article | Journal: Journal of enzyme inhibition and medicinal chemistry | Year: 2016
The currently available therapies for type 2 diabetes have been unable to achieve normoglycemic status in the majority of patients. The reason may be attributed to the limitations of the drug itself or its side effects. In an effort to develop potent and safe oral antidiabetic agents, we evaluated the in vitro and in vivo hypoglycemic effects of 10 synthetic polyphenolic curcumin analogues on alloxan-induced male diabetic albino rats. In vitro studies showed 7-bis(3,4-dimethoxyphenyl)hepta-1,6-diene-3,5-dione (4) to be the most potential hypoglycemic agent followed by 1,5-bis(4-hydroxy-3-methoxyphenyl)penta-1,4-dien-3-one (10). Structure activity relationship (SAR) of the tested compounds was elucidated and the results were interpreted in terms of in vitro hypoglycemic activities. Furthermore, oral glucose tolerance test (OGTT) with compounds 4, 10 and reference hypoglycemic drug glipizide showed that compound 4 and glipizide had relatively similar effects on the reduction of blood glucose levels within 2h. Thus, compound 4 might be regarded as a potential hypoglycemic agent being able to reduce glucose concentration both in vitro and in vivo.
Salmani Manjunath P.,Shri BM Patil Medical College |
Mindolli P.B.,Al Ameen Medical College
Journal of Communicable Diseases | Year: 2011
A rapid test for diagnosis of malaria based on acridine orange staining of centrifuged blood samples in a microhaematocrit tube (QBC) was compared with Leishman stained thin peripheral blood smear in 287 samples. Malaria was diagnosed in 44 patients by Leishman staining technique and in 65 patients by QBC method. The QBC method allowed detection of an additional 21 cases. Thus the prevalence rate of malaria during the study was 22.65%. In 222 Patients who were negative by the QBC technique, the Leishman stained smears were also negative for malarial parasite. Although QBC method was superior to the smear for malarial parasite detection, species identification was difficult by this technique. The QBC method provides a reliable, quick, easily mastered, accurate method for diagnosis of malaria. The QBC system can also be used in the diagnosis of other parasitic diseases from blood (Filariasis). However, Leishman stained thin blood film still appear superior for species identification.
Mujawar Q.M.,Al Ameen Medical College |
Mujawar Q.M.,King Khalid University |
Naganoor R.,Al Ameen Medical College |
Ali M.D.,Guru Nanak Hospital and Research Center |
And 2 more authors.
Journal of Tropical Pediatrics | Year: 2012
Objective: To determine the effects and safety of dioctahedral smectite (DS) on the duration of acute watery diarrhea in children. Methods: A Randomized, open labeled, clinical controlled trial in a tertiary care hospital outpatient department (OPD) and emergency department. Participants were one hundred and seventeen children without any chronic illness between 2 and 5 years presenting to OPD, having acute watery diarrhea for <48 h with mild to moderate dehydration, not on antibiotics and requiring oral rehydration therapy. Intervention done was DS with a dose of 1.5 g thrice daily. Results: Freshly dissolved DS in a dose of 1.5 g thrice daily for 5 days significantly shortened the duration of acute watery diarrhea in children aged 2-5 years. There were no adverse effects on the use of DS. DS was acceptable to the children, and its administration was not accompanied with any side effects. Conclusion: DS reduces the duration of diarrhea in Indian children and prevents a prolonged course, and therefore, may consistently reduce the costs in treatment of acute watery diarrhea.© The Author . Published by Oxford University Press. All rights reserved.
Das K.K.,BLDE University |
Jargar J.G.,Al Ameen Medical College |
Saha S.,University of Leeds |
Yendigeri S.M.,Al Ameen Medical College |
Singh S.B.,Defence Institute of Physiology and Allied science
Indian Journal of Pharmacology | Year: 2015
Objective: Lead (Pb) is a long-known poison of environment and industrial origin. Its prolonged exposure affects cellular material and alters cellular genetics and produces oxidative damages. In this study, we investigated the exposure of chronic sustained hypoxia or lead acetate alone or in combination with or without supplementation of α-tocopherol on hepatic oxidative and nitrosative stress in rats. Materials and Methods: The rats weighing 165 ± 5 g were exposed to chronic sustained hypoxia (10% oxygen) or lead acetate (25 mg/kg of body weight, intraperitoneally) alone or in combination with or without supplementation of α-tocopherol (10 mg/100 g b.wt, intramuscularly). The body weight of all the rats was recorded on the day 1 of the treatment and the day of sacrifice. Serum lipid profile was estimated by using a biochemical analyzer. Oxidant and enzymatic antioxidants status was evaluated by using spectrophotometer. Serum levels of hypoxia inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) were measured by using ELISA technique. Histopathological assessments of hepatic tissue were also done. Results: Exposure of both lead and hypoxia showed decreased body weight, altered serum lipid profile, oxidant and enzymatic antioxidants status, serum HIF-1α and VEGF concentrations. Simultaneous α-tocopherol supplementation showed beneficial effects to all these alterations. Histopathological observations also showed hepatic degenerative changes after lead or hypoxia exposure either alone or in combination, but remarkable improvement has been noticed after α-tocopherol supplementation. Conclusion: Supplementation of α-tocopherol is beneficial to counter both lead acetate and hypoxia induced hepatic cytotoxicities possibly by reducing oxidative and nitrosative stress.
Bhuyar B.K.,D Y Patil Medical College |
Shamsuddin M.,Al Ameen Medical College
International Journal of Pharma and Bio Sciences | Year: 2014
The current theory suggests that in preeclampsia there is an increase in the lipid peroxidation products and leads to a decrease in the plasma antioxidants except uric acid.Along with this change in the lipid profile level leads to the pathogenesis of preeclampsia.In this context, this study was undertaken to determine the changes in plasma levels of lipid peroxide, antioxidant levels in women with preeclampsia and to investigate the effect on lipid profile. To measure the levels of serum lipid profile in preeclampsia in comparison with normal pregnancy. Cross sectional study consisting of 30 preeclamptic and 30 healthy pregnant women. Fasting venous blood samples were collected during antepartum period and plasma levels of TG, TC, HDL-C, VLDL-C, and LDL-C were measured.: In the preeclamptic group, there was a significant increase in the triglyceride levels, but there were no significant changes in other lipid profile parameters. The findings of the present study are consistent with previous studies, lipid profile levels are important factors in the pathogenesis of preeclampsia. In preeclampsia plasma antioxidants are excessively utilized to counteract the cellular changes mediated by free radicals.
Bhagat S.S.,Al Ameen Medical College |
Ghone R.A.,A.C.P.M.C. Dhule |
Suryakar A.N.,Dr Vm Government Medical College |
Hundekar P.S.,A.C.P.M.C. Dhule
Indian Journal of Physiology and Pharmacology | Year: 2011
Reactive Oxygen Species (ROS) can induce carcinogenesis via DMA injury. Both enzymatic and non-enzymatic antioxidants participate in cell protection against harmful influence of oxidative stress. The aim of the present study was to assess the level of end product of lipid peroxidation such as malondialdehyde (MDA) as an oxidant in colorectal cancer. Moreover, we analyzed the activity of main non-enzymatic antioxidants, vitamin E and vitamin C in colorectal cancer patients. In the present study, total 48 samples were analyzed which includes 24 age matched healthy controls irrespective of sex (Group I) and 24 cases of colorectal cancer (Group II). The serum level of lipid peroxide, vitamin E and vitamin C were estimated in both healthy control Group I (n=24) and colorectal cancer Group II (n=24). A significant increase in the level of serum lipid peroxide (P<0.001), with concomitant decrease in the levels of serum vitamin E and vitamin C, (P<0.001) in Group II patients were noticed as compared to the healthy control Group I. Our findings suggest that increased oxidative stress and reduced antioxidants defense mechanism may play an important role in progression and pathogenesis of colorectal cancer.
Huilgol S.V.,Al Ameen Medical College |
Kumar V.H.,Al Ameen Medical College
Journal of Basic and Clinical Physiology and Pharmacology | Year: 2014
Background: Oxidative free radicals and lipid peroxidation mediate gastric injury. α-Tocopherol is a redox agent with biological and antioxidant property, hence, may provide ulcer protection. Methods: Pylorus-ligated Shay rats (n =6) were used as the experimental gastric ulcer animal model. The rats were divided into three groups. Group I received saline (5 mL/kg), Group II α-tocopherol (12.5 mg/kg), and Group III omeprazole (3.6 mg/kg), orally daily for 5 days prior to ulcerogenic challenge. Nineteen hours after the challenge, the rats were sacrificed and their stomachs isolated and studied for degree of gastric injury. Formed gastric juice was collected for measurement of volume, titrimetric estimation of free and total acidity, and total acid output by the conventional methods. The ulcer index and total acid outputs were calculated. Results: α-Tocopherol exerted significant (p 0.05) antiulcer activity (the ulcer index was reduced to 7.4 ±1.0 from the control value of 19.8 ±4.1). α-Tocopherol also significantly reduced free and total acidity, gastric juice volume, and total acid output (p <0.01). The results were analyzed by ANOVA and Scheffe's multiple comparison test. Conclusions: The study demonstrates that ?-tocopherol has significant antiulcer activity. It, perhaps, acts by decreasing hydrochloric acid output.
Bajaj R.,University of Massachusetts Medical School |
Chhabra L.,University of Massachusetts Medical School |
Basheer Z.,Al Ameen Medical College |
Spodick D.H.,University of Massachusetts Medical School
International Journal of COPD | Year: 2013
Background: Pulmonary emphysema of any etiology has been shown to be strongly and quasidiagnostically associated with a vertical frontal P wave axis. A vertical P wave axis (.60 degrees) during sinus rhythm can be easily determined by a P wave in lead III greater than the P wave in lead I (bipolar lead set) or a dominantly negative P wave in aVL (unipolar lead set). The purpose of this investigation was to determine which set of limb leads may be better for identifying the vertical P vector of emphysema in adults. Methods: Unselected consecutive electrocardiograms from 100 patients with a diagnosis of emphysema were analyzed to determine the P wave axis. Patients aged younger than 45 years, those not in sinus rhythm, and those with poor quality tracings were excluded. The electrocardiographic data were divided into three categories depending on the frontal P wave axis, ie,.60 degrees, 60 degrees, or,60 degrees, by each criterion (P amplitude lead III. lead I and a negative P wave in aVL). Results: Sixty-six percent of patients had a P wave axis. 60 degrees based on aVL, and 88% of patients had a P wave axis. 60 degrees based on the P wave in lead III being greater than in lead I. Conclusion: A P wave in lead III greater than that in lead I is a more sensitive marker than a negative P wave in aVL for diagnosing emphysema and is recommended for rapid routine screening. © 2013 Bajaj et al, publisher and licensee Dove Medical Press Ltd.
PubMed | P.A. College and Al Ameen Medical College
Type: Journal Article | Journal: Journal of the Indian Society of Pedodontics and Preventive Dentistry | Year: 2017
Dental caries is one of the most prevalent infectious diseases to affl ict humanity. Although caries has multifactorial etiology, inherited genetic behavior and taste threshold may play an important role on caries.Thirty mothers and thirty children in the age group of 6-14 years of both sexes who have stable mental condition and ASA physical status were selected for the study & 6-n-propylthiouracil testing is done.It is observed that nontaster siblings have higher caries prevalence than medium tasters and supertasters.Genetic sensitivity to taste is an inherited trait in children from their parents, inheritance from mother being more pronounced. Hence, this study is intended.Dental caries is multi-factorial. No significant correlation between susceptibility of mother and child to genetic sensitivity exists, and genetic sensitivity is not the only criteria for severity.
PubMed | Al Ameen Medical College
Type: Journal Article | Journal: Indian journal of physiology and pharmacology | Year: 2016
Aspartate amino transferase (GOT) and alanine amino transferase (GPT) activities were studied in plasma, liver and kidney of aspirin treated and ascorbic acid supplemented groups for a period of seven days. GOT and GPT activities were increased in plasma but decreased significantly in liver and kidney in aspirin treated animals. Ascorbic acid supplemented groups showed no significant change of GOT and GPT in plasma and liver. In case of kidney, GOT activity remained unchange but GPT activity showed significant change in ascorbic acid supplemented group. The results clearly indicate that aspirin is a potent hepatotoxic and nephrotoxic drug but supplementation of ascorbic acid in High doses to rats fed aspirin can restore enzyme activities to the normal level.