New Market, MD, United States
New Market, MD, United States

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Patent
Akonni Biosystems Inc. and Bio Rad Laboratories Inc. | Date: 2016-09-14

A Method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.


Patent
Akonni Biosystems Inc. | Date: 2014-06-03

A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.


Patent
Akonni Biosystems Inc. | Date: 2015-12-22

A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.


Patent
Akonni Biosystems Inc. | Date: 2016-07-20

Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter.


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase II | Award Amount: 1.20M | Year: 2013

DESCRIPTION provided by applicant Of all diseases tuberculosis TB represents one of if not the greatest health disparity between whites and minorities For every TB infected white person in the United States there are an estimated African Americans Latinos Native Americans Asians and Native Hawaiian Pacific Islanders with this disease Compounded with this disparity is the prevalence of drug resistant mutations of TB which have an associated polymorphisms that span genes two promoter regions and one ribosomal RNA coding region Current methodologies available primarily to affluent healthcare communities utilize microbial cultures which require sophisticated laboratories and weeks before a result can be determined Difficulties for minorities in a low socioeconomic class to commute and or follow up with their physicians can result in a lack of appropriate treatment A low cost simple and rapid point of care POC test that detects TB and its resistance to first line drugs isoniazid rifampin ethambutol and pyrazinamide would improve TB diagnostics for these minority communities However current technologies lack sensitivity specificity and or multiplexing capacity to achieve this goal We therefore propose to develop a POC device that offers the sensitivity of culture methods specificity of nucleic acid methods and a broad coverage of mutations To accomplish this we will advance our TruArray platform hemispherical porous gel drop microarrays for TB diagnostics using on chip PCR and our existing MDR TB PCR microarray biochips During Phase we demonstrated feasibility of our sample preparation approach our PCR micorarray biochips and prototype POC instrument For Phase we propose to expand the coverage of our test to include additional mutations that confer resistance to all first line drugs integrate this test onto our POC instrument and translae this system to Laboratorios Medicos Especializados in Juarez Mexico PUBLIC HEALTH RELEVANCE Of all diseases Tuberculosis TB represents one of if not the greatest health disparity between whites and minorities To be specific for every TB infected white person in the United States there are an estimated African Americans Latinos Native Americans Asians and Native Hawaiian Pacific Islanders with this disease The proposed project is to develop a point of care device for identifying drug resistant strains of Tuberculosis that can be widely disseminated to minority populations


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase II | Award Amount: 1.50M | Year: 2015

DESCRIPTION provided by applicant Sample preparation frequently presents a bottleneck for clinical molecular biology workflows and diagnostic tests Whereas there are many molecular detection modalities there are only a handful of automated sample preparation workflow strategies Existing instruments that are built around these sample preparation strategies and chemistries range in cost from $ $ k and yet they still do not include an integrated method for lysing difficult to disrupt organisms such as gram positive bacteria or the acid fast bacilli e Mycobacterium To address these limitations we will integrate Akonniandapos s sample preparation technologies into a low cost automated instrument that can process a variety of sample types with simple to use consumable kits Based on Phase results the two year goal for Phase is to demonstrate the efficacy of pre production integrated sample preparation systems with a disposable M tuberculosis sputum DNA extraction kit in a targeted intended use setting a clinic with a moderate to high TB burden rate PUBLIC HEALTH RELEVANCE Molecular diagnostics which are tests that analyze the DNA and or RNA in a sample are becoming increasingly prevalent among clinical laboratory test methods An important yet challenging procedure required of these tests is to remove the DNA RNA from the sample and wash away the impurities We seek to develop a low cost automated instrument to address this challenge


Patent
Akonni Biosystems Inc. | Date: 2015-12-01

An integrated sample purification system includes a housing, a sample container rack, a filter holder, and a cylindrical magnet. The sample container rack and the filter device holder are disposed in the housing. The sample container rack is configured to hold one or more sample containers, the filter device holder is configured to hold one or more filter devices. The cylindrical magnet is adjacent to and external to the sample container rack, and is rotated about a central, longitudinal axis of the magnet by an electric motor disposed in the housing to lyse cells. Molecules of interest in the lysed cells are purified using filters that bind specifically to the molecules of interest. The system is readily amenable to automation and rapid purification and analysis of molecules of interest, such as nucleic acids and proteins.


Patent
Akonni Biosystems Inc. | Date: 2015-10-26

A positioning system for a sample analysis device is disclosed. The positioning system comprises (1) a carousel comprising a platform and a sample loading tray mounted on the platform, and (2) a stage comprising a positioning system for positioning the carousel under the optical path of an imaging system. The sample loading tray is configured for holding a cartridge comprising one or more lateral flow cells (LFCs).


Patent
Akonni Biosystems Inc. | Date: 2016-09-29

A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.


Patent
Akonni Biosystems Inc. | Date: 2015-06-18

A molecular testing device comprises a heating and cooling module having a thin-film thermoelectric heating and cooling device, and a removable test module having a combined amplification and hybridization reaction chamber. The reaction chamber comprises a thermo-conductive exterior surface and a microarray on an interior surface. The thin-film thermoelectric heating and cooling device has a heat transfer surface that is adapted to make contact with the thermo-conductive exterior surface of the reaction chamber. The molecular testing device may be used to perform a PCR in the reaction chamber.

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