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Imamura C.,TOYOTA CENTRAL R and D LABS. INC. | Shigemori Y.,Aisin Cosmos RandD Co.
Bioscience, Biotechnology and Biochemistry | Year: 2010

We used directed evolution to enhance the thermostability of formaldehyde dehydrogenase from Pseudo-monas putida. At 50°C, the wild-type enzyme was inactivated within 30min, but the variants obtained retained 80% activity for at least 300 min. At room temperature (30°C), the variants obtained retained <80% activity for at least 500 h (21 d). Source


Murata K.,University of Tsukuba | Shigemori Y.,Aisin Cosmos RandD Co. | Tsujimura S.,University of Tsukuba
Chemistry Letters | Year: 2015

Here, we report a novel electrochemical activation technique for a Cu-depleted apo-type laccase, MELAC, which was isolated from a compost metagenome. The Cu atoms in the active center of the laccase were removed during the purification process using an anion-exchange column. We demonstrate how the apotype laccase can be activated by an electrochemically generated monovalent Cu+ ion. © 2015 The Chemical Society of Japan. Source


Takahashi Y.,Hiroshima University | Kondo K.,Aisin Cosmos RandD Co. | Miyaji A.,Hiroshima University | Umeo M.,Hiroshima University | And 2 more authors.
Analytical Sciences | Year: 2012

Given that the supply of several rare earth elements (REEs) is sometimes limited, recycling REEs used in various advanced materials, such as Nd magnets, is important for realizing efficient use of REE resources. In the present work, the feasibility of using DNA for REE recovery and separation was examined, along with the identification of the binding site of REEs in DNA. In particular, a DNA-cellulose filter paper hybrid was prepared so that DNA-based materials can be used for the separation of REEs using columns loaded with DNA. N, N'-Disuccinimidyl was used as a cross-linker reagent for the fixation of DNA onto a fibrous cellulose filter. The results showed that (i) the DNA-filter hybrid has a sufficiently high affinity to adsorb REEs; (ii) the adsorption capacity was 0.182 mg/g for Nd; and (iii) the affinity of REEs for DNA was stronger for REEs with larger atomic numbers. The difference of the affinity among REEs in the third result was compared with the adsorption patterns of REEs discussed in the literature. The comparison suggests that phosphate in the DNA-filter paper hybrid was responsible for REE adsorption onto the hybrid. The results were supported by the Nd, Dy, and Lu LIII-edge EXAFS; the REE-P shell was identified for the second neighboring atom, showing the importance of the phosphate site as REE binding sites. The difference in the affinity among REEs suggest that group separation of REEs (such as La, Ce, (Pr and Nd), (Ho, Dy, and Er), (Tb and Gd), (Sm, Eu), Tm, Yb, and Lu) is possible, although complete isolation of each REE from a solution containing all REEs may be difficult. For practical applications, Nd and Fe(III) were successfully separated from a synthetic solution of Nd magnet waste using columns loaded with the DNAfilter hybrid. © 2012 The Japan Society for Analytical Chemistry. Source


Wakui H.,Aisin Cosmos RandD Co. | Hirata Y.,Chubu University
Transactions of Japanese Society for Medical and Biological Engineering | Year: 2013

Reduction in arousal is one of the main causes that lead to lapses of driver's attention. Therefore, in order to keep a safe drive by the operation of driver's own, it is important to detect the reduced arousal as soon as possible. Currently we focused on saccadic eye movements, which can be measured noninvasively, as a possible detector of driver's reduced arousal. In this paper, we evaluated saccades while subjects were performing a simple reaction test and a driving simulation. We found that changes in characteristics of horizontal saccades, i. e. peak velocities and inter-saccadic intervals, were synchronized with the fluctuation of pupil area that is a well known objective measure of arousal level. Further, when the pupil area decreased because of reduced arousal, significant reductions of these saccade parameters were found prior to the occurrence of slow eye movement (SEM) that is another indicator of unconsciousness or aimlessness. Based upon these findings, we conclude that slowed peak horizontal saccade velocity (<40 deg/s) and shortened inter-saccadic interval (<0.2 s) are useful detector of driver's reduced arousal. Source


Giribabu L.,Indian Institute of Chemical Technology | Bessho T.,Institute of Chemical science and Engineering | Srinivasu M.,Indian Institute of Chemical Technology | Vijaykumar C.,Indian Institute of Chemical Technology | And 6 more authors.
Dalton Transactions | Year: 2011

Two new heteroleptic ruthenium(ii) photosensitizers that contains 2,2′;6,2′′-terpyridine with extended π-conjugation with donor groups, a 4,4′-dicarboxylic acid-2,2′-bipyridine anchoring ligand and a thiocyanate ligand have been designed, synthesized and fully characterized by CHN, mass spectrometry, UV-vis and fluorescence spectroscopies and cyclic voltammetry. The new sensitizers have either 3,5-di-tert-butyl phenyl (m-BL-5) or triphenylamine (m-BL-6) groups, where the molar extinction coefficient of both the sensitizers is higher than the analogous ruthenium dyes. Both the sensitizers were tested in dye-sensitized solar cells using two different redox electrolytes. © 2011 The Royal Society of Chemistry. Source

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