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PubMed | China Tianjin Institute of Hygiene and Environmental Medicine, Guangxi Medical University, General Hospital of Airforce, Tianjin Institute of Hygiene and Environmental Medicine and Center for Identification of Chinese Herbal Medicines
Type: Journal Article | Journal: Molecular biology of the cell | Year: 2016

We describe a novel functional interaction between ASK1 and PRMT5. We show that PRMT5 interacts with and methylates ASK1 at arginine residue 89 and thereby negatively regulates its activity by promoting the interaction between ASK1 and Akt and thus phosphorylating ASK1 at serine residue 83. Furthermore, the association between ASK1 and Akt is enhanced by VEGF stimulation, and PRMT5 is required for this association. Moreover, PRMT5-mediated ASK1 methylation impaired the H2O2-induced activity of ASK1, and this inhibitory effect of PRMT5 was abolished by replacement of arginine 89 with Trp or depletion of PRMT5 expression by RNA interference. Together the results demonstrate cross-talk between arginine methylation and serine phosphorylation in ASK1.


Du L.,Beijing Institute of Radiation Medicine | Du L.,Beijing Institute of Pharmacology and Toxicology | Mei X.,Beijing Institute of Pharmacology and Toxicology | Wang C.,General Hospital of PLA | And 3 more authors.
Anti-Cancer Drugs | Year: 2011

The introduction of luteinizing hormone-releasing hormone (LHRH) analogs and their antagonists is revolutionizing the treatment of prostate cancer. In this study, poly(d,l-lactide-co-glycolide) (PLGA) microspheres containing a highly potent LHRH antagonist (LXT-101) of interest in the indication of prostate cancer were evaluated on release mechanisms in vitro and biological performance in vivo. LXT-101 microspheres were prepared by the water/oil/water double emulsion method and the solid/oil/oil method. The results showed that the mechanism of LXT-101 releasing from PLGA 14000 microspheres was the cooperation of drug diffusion and polymer degradation. This clarified the relationship between the microsphere characterization and hormone level in vivo. The larger microspheres (33 μm) could inhibit the testosterone level to castration for a longer time (35 days) than the smaller microspheres (15 μm, 14 days). The formulation containing the hydrophilic additive (polyethylene glycol 6000) could suppress the testosterone level to castration for a longer time (>35 days) than the formulation without polyethylene glycol (14 days). The appearance of testis, vesicular seminalis, and prostates changed after treatment. The weights of sexual organs decreased significantly. The in-vivo release of the LXT-101 PLGA 14000 microspheres curve showed that in-vivo release started immediately after day 1 (22.7%) and was rapid during the first 5 days (40.2% release). The LXT-101 microspheres could be a promising drug delivery system candidate to treat sex hormone-dependent tumors and other related disorders. © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.


Zhu Y.-J.,General Hospital of Airforce | Xu B.,Anhui Medical University | Xia W.,Chinese Institute of Basic Medical Sciences
Clinical Laboratory | Year: 2014

Background: This work was undertaken to identify the differences in expression of miRNAs, which would predict early-stage non-small cell lung cancer prognosis and recurrence. The results may be helpful to demonstrate one mechanism of lung squamous cancer proliferation. Methods: Lung squamous cell carcinoma specimens were collected at the time of surgery. Microarray of expression of specific miRNAs in lung squamous cell cancer tissue, were assessed by qRT-PCR. Results: We found that the hsa-mir-182 family are highly expressed in lung squamous cell carcinoma. Paradoxically, our study reveals mir-182 suppresses cell proliferation in vivo. RASA1 is related to cell apoptosis. We further show that mir-182 downregulates RASA1. Conclusions: We provide first time evidence through tissue microarray and quantitative PCR validation of mir-182 in the expression of lung squamous cell carcinoma. Our data provide a possible mechanism for lung cancer cell proliferation in lung squamous cell cancer and may be helpful in discovering a new strategy to reveal lung squamous cell carcinoma progress.


Xu B.,General Hospital of Airforce | Xia W.,Chinese Institute of Basic Medical Sciences | Shao N.-S.,Chinese Institute of Basic Medical Sciences | Zhu Y.-J.,General Hospital of Airforce
Chinese Journal of Cancer Prevention and Treatment | Year: 2013

OBJECTIVE: To detect the different expression of microRNA (miRNA) in lung squamous cell cancer and to predict and validate their biological function. METHODS: Microarray was used to discover the special miRNA which was differentially expressed between lung squamous cell cancer tissues and normal lung tissues. The mir-182 family which was significantly upregulated in lung squamous cell cancer tissues were tested through qualitative PCR. The target genes were predicted by public algorithms and confirmed in vivo. RESULTS: Hsa-mir-182 family was highly expressed in lung squamous cell carcinoma. After mir-182 being overexpressed, the expression of RASA1 was significantly down regulated, which further confirmed that RASA1 was the target gene of mir-182. CONCLUSIONS: Hsa-mir-182 family is significantly high expressed in lung squamous cell cancer. Mir-182 downregulates RASA1 and it may play an important role in lung squamous cell cancer.


Chen M.,Center for Identification of Chinese Herbal Medicines | Chen M.,Tianjin Institute of Hygiene and Environmental Medicine | Qu X.,Center for Identification of Chinese Herbal Medicines | Zhang Z.,Tianjin Institute of Hygiene and Environmental Medicine | And 7 more authors.
Molecular Biology of the Cell | Year: 2016

We describe a novel functional interaction between ASK1 and PRMT5. We show that PRMT5 interacts with and methylates ASK1 at arginine residue 89 and thereby negatively regulates its activity by promoting the interaction between ASK1 and Akt and thus phosphorylating ASK1 at serine residue 83. Furthermore, the association between ASK1 and Akt is enhanced by VEGF stimulation, and PRMT5 is required for this association. Moreover, PRMT5-mediated ASK1 methylation impaired the H2O2-induced activity of ASK1, and this inhibitory effect of PRMT5 was abolished by replacement of arginine 89 with Trp or depletion of PRMT5 expression by RNA interference. Together the results demonstrate cross-talk between arginine methylation and serine phosphorylation in ASK1. © 2016 Billmann, Horn, et al.


Xu J.,PLA Fourth Military Medical University | Li J.,No 323 Hospital Of Pla | Wu X.,PLA Fourth Military Medical University | Song C.,General Hospital of Airforce | And 9 more authors.
Protein Expression and Purification | Year: 2015

In order to obtain bioactive α-bungarotoxin (αBtx) using recombinant protein technique, a codon-optimized synthetic gene was expressed in fusion with the N-terminal 10-His-tag and C-terminal Strep-tag in Escherichia coli. Further optimization through site-directed mutagenesis enabled moderate expression of the protein without the N-terminal His-tag or the C-terminal Strep-tag. Two such recombinant αBtx (rαBtx) were obtained, both with an additional methionine and a glycine at the N-terminal and one with (G4S1)2-Strep-tag at the C-terminal. The rαBtx proteins were refolded using a novel protocol, which efficiently produced final products with activity similar to its natural counterpart. The protocol could easily be scale up, which produced 0.3-1 mg of pure and highly active rαBtx per liter of E. coli culture. © 2015 Elsevier Inc. All rights reserved.


Feng W.,General Hospital of Airforce
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2013

To observe three-dimensional space position change of nucleus pulposus and nerve root before and after treatment of lumbar disc herniation by spinal fixed-point rotating reduction, and explore the mechanisms. Totally 52 patients with L5S1 lumbar disc hernation treated by spinal fixed-point rotating reduction were collected from April 2009 to June 2011. There were 33 males and 19 females with an average age of 34.6 years old (ranged, 19 to 55). Three-dimensional MRI were performed to observe relationship between nucleus pulposus and related nerve root,configuration change of spine and pelvic on coronary MRI. MRI showed relationship between nucleus pulposus and related nerve root mainly located on axillary, shoulder, front and surround. Vertebral displacement disappeared, lumbocrural pain alleviated after manipulative therapy. All patients were followed up from 2 to 28 months with an average of 12 months, and no recurred. All patients recovered work. Nucleus pulposus had no change,while lumbral spinal and pelvic curve changed before and after admission. Lumbar disc herniation combined with single (multiple) vertebral displacement,can cause biomechanical properties of nucleus puplosus and related nerve root, while spinal fixed-point rotating reduction can correct vertebral displacement, recover balance between inside and outside of spinal.


Qi Y.,Chinese Institute of Aviation Medicine | Feng J.,General Hospital of AirForce | Guo H.,Chinese Institute of Aviation Medicine | Jing B.,Chinese Institute of Aviation Medicine
Lecture Notes in Electrical Engineering | Year: 2014

The objective of this study is to investigate blood routine changes of aerotransport crew and mice under the microwave radiation (MW) exposure for health promotion strategy. According to their duty, airfield staff were divided into two groups: radar operator group (N = 25); and common worker group (n = 48), and their blood was collected for blood routine analysis. Thirty Kunming male mice were randomly divided into three groups (n = 10): the sham MW group, MW one-time group, and MW three-time group. Microwave radiation conditions are as follows: average surface density of MW is 10 mW/cm2 and the frequency of MW exposure is once every two days and 15 min every time. After the final MW exposure, the blood of mice was collected for blood routine analysis. Results: compared with common worker group, the case of low WBC quantity in radar operator group increased significantly (P < 0.05); and compared with the sham MW group, RBC quantity of MW three-time group decreased obviously (P < 0.05). Conclusion: MW radiation can injure WBC of radar operators or RBC of mice, and it may threat the health of airfield staff. © 2014 Springer-Verlag.


PubMed | General Hospital of Airforce
Type: Journal Article | Journal: Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2013

To observe three-dimensional space position change of nucleus pulposus and nerve root before and after treatment of lumbar disc herniation by spinal fixed-point rotating reduction, and explore the mechanisms.Totally 52 patients with L5S1 lumbar disc hernation treated by spinal fixed-point rotating reduction were collected from April 2009 to June 2011. There were 33 males and 19 females with an average age of 34.6 years old (ranged, 19 to 55). Three-dimensional MRI were performed to observe relationship between nucleus pulposus and related nerve root,configuration change of spine and pelvic on coronary MRI.MRI showed relationship between nucleus pulposus and related nerve root mainly located on axillary, shoulder, front and surround. Vertebral displacement disappeared, lumbocrural pain alleviated after manipulative therapy. All patients were followed up from 2 to 28 months with an average of 12 months, and no recurred. All patients recovered work. Nucleus pulposus had no change,while lumbral spinal and pelvic curve changed before and after admission.Lumbar disc herniation combined with single (multiple) vertebral displacement,can cause biomechanical properties of nucleus puplosus and related nerve root, while spinal fixed-point rotating reduction can correct vertebral displacement, recover balance between inside and outside of spinal.

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