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Li D.X.,Air Force General Hospital PLA | Deng T.Z.,Air Force General Hospital PLA | Lv J.,Air Force General Hospital PLA | Ke J.,Air Force General Hospital PLA
Brazilian Journal of Medical and Biological Research | Year: 2014

Diabetics have an increased prevalence of periodontitis, and diabetes is one of the causative factors of severe periodontitis. Apoptosis is thought to be involved in this pathogenic relationship. The aim of this study was to investigate apoptosis in human periodontal ligament (PDL) fibroblasts induced by advanced glycation end products (AGEs) and their receptor (RAGE). We examined the roles of apoptosis, AGEs, and RAGE during periodontitis in diabetes mellitus using cultured PDL fibroblasts that were treated by AGE-modified bovine serum albumin (AGE-BSA), bovine serum albumin (BSA) alone, or given no treatment (control). Microscopy and real-time quantitative PCR indicated that PDL fibroblasts treated with AGE-BSA were deformed and expressed higher levels of RAGE and caspase 3. Cell viability assays and flow cytometry indicated that AGE-BSA reduced cell viability (69.80±5.50%, P,0.01) and increased apoptosis (11.31±1.73%, P,0.05). Hoechst 33258 staining and terminaldeoxynucleotidyl transferase-mediated nick-end labeling revealed that AGE-BSA significantly increased apoptosis of PDL fibroblasts. The results showed that the changes in PDL fibroblasts induced by AGE-BSA may explain how AGE-RAGE participates in and exacerbates periodontium destruction. © 2014, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.

Deng T.,Air Force General Hospital PLA | Lv J.,Air Force General Hospital PLA | Pang J.,Air Force General Hospital PLA | Liu B.,Air Force General Hospital PLA | Ke J.,Air Force General Hospital PLA
Journal of Tissue Engineering and Regenerative Medicine | Year: 2014

In this study, a novel three-dimensional (3D) heterogeneous/bilayered scaffold was constructed to repair large defects in rabbit joints. The scaffold includes two distinct but integrated layers corresponding to the cartilage and bone components. The upper layer consists of gelatin, chondroitin sulphate and sodium hyaluronate (GCH), and the lower layer consists of gelatin and ceramic bovine bone (GCBB). The two form a 3D bilayered scaffold (GCH-GCBB), which mimics the natural osteochondral matrix for use as a scaffold for osteochondral tissue engineering. The purpose of this study was to evaluate the efficacy of this novel scaffold, combined with chondrocytes and bone marrow stem cells (BMSCs) to repair large defects in rabbit joints. Thirty-six large defects in rabbit femoral condyles were created; 12 defects were treated with the same scaffold combined with cells (group A); another 12 defects were treated with cell-free scaffolds (group B); the others were untreated (group C). At 6 and 12weeks, in group A hyaline-like cartilage formation could be observed by histological examination; the newly formed cartilage, which stained for type II collagen, was detected by RT-PCR at high-level expression. Most of the GCBB was replaced by bone, while little remained in the underlying cartilage. At 36weeks, GCBB was completely resorbed and a tidemark was observed in some areas. In contrast, groups B and C showed no cartilage formation but a great amount of fibrous tissue, with only a little bone formation. In summary, this study demonstrated that a novel scaffold, comprising a top layer of GCH, having mechanical properties comparable to native cartilage, and a bottom layer composed of GCBB, could be used to repair large osteochondral defects in joints. © 2012 John Wiley & Sons, Ltd.

Deng T.-Z.,Air Force General Hospital PLA | Lu J.,Air Force General Hospital PLA | Feng Y.,Air Force General Hospital PLA | Li D.-X.,Air Force General Hospital PLA | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: It is the focus and hotspot for the research of tissue engineering scaffold materials to prepare substitutes for the extracellular matrix with cell recognition and bionic scaffolds. Objective: To prepare and filter a porous three-dimensional scaffold for osteochondral tissue engineering which can meet the requirement and to evaluate its biocompatibility. Methods: Four kinds of scaffolds: collagen-chitosan, gelatin-chondroitin-sulfuric acid-sodium hyaluronate, collagen-ceramic bone and gelatin-ceramic bone were made by different biomaterials and divided into 1, 2, 3 and 4 groups, respectively. The fresh articular cartilage served as the control group. Results and Conclusion: In group 1, bore diameter was 50-200 μm, and porosity was (90.5±2.1)%. In group 2, bore diameter was 100-150 μm, and porosity was (90.5±2.1)%. In group 3, bore diameter was 400-500 μm, and porosity was (67.5±2.1)%. In group 4, bore diameter was 300-400 μm, and porosity was (65.9±1.2)%. Mechanical test showed that the group 2 and group 4 basically met the experimental requirement: the structure and biochemistry components in groups 2, 3 and 4 were similar to the natural extracellular matrix, and could imitate extracellular microenvironment. These findings suggest that the gelatin-chondroitin-sulfuric acid-sodium hyaluronate and gelatin- ceramic bone scaffolds can be used as the scaffolds for composite tissues.

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