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Zhou Y.,Air Force General Hospital | Liu C.-H.,City University of New York | Sun Y.,City University of New York | Pu Y.,City University of New York | And 3 more authors.
Journal of Biomedical Optics | Year: 2012

The resonance Raman (RR) spectra of six types of human brain tissues are examined using a confocal micro-Raman system with 532-nm excitation in vitro. Forty-three RR spectra from seven subjects are investigated. The spectral peaks from malignant meningioma, stage III (cancer), benign meningioma (benign), normal meningeal tissues (normal), glioblastoma multiforme grade IV (cancer), acoustic neuroma (benign), and pituitary adenoma (benign) are analyzed. Using a 532-nm excitation, the resonance-enhanced peak at 1548 cm-1 (amide II) is observed in all of the tissue specimens, but is not observed in the spectra collected using the nonresonance Raman system. An increase in the intensity ratio of 1587 to 1605 cm-1 is observed in the RR spectra collected from meningeal cancer tissue as compared with the spectra collected from the benign and normal meningeal tissue. The peak around 1732 cm -1 attributed to fatty acids (lipids) are diminished in the spectra collected from the meningeal cancer tumors as compared with the spectra from normal and benign tissues. The characteristic band of spectral peaks observed between 2800 and 3100 cm-1 are attributed to the vibrations of methyl (-CH3) and methylene (-CH2-) groups. The ratio of the intensities of the spectral peaks of 2935 to 2880 cm-1 from the meningeal cancer tissues is found to be lower in comparison with that of the spectral peaks from normal, and benign tissues, which may be used as a distinct marker for distinguishing cancerous tissues from normal meningeal tissues. The statistical methods of principal component analysis and the support vector machine are used to analyze the RR spectral data collected from meningeal tissues, yielding a diagnostic sensitivity of 90.9% and specificity of 100% when two principal components are used. © 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).

Gao Y.,Chinese PLA General Hospital | Gao Y.,Shanghai University | Meng H.,Air Force General Hospital | Liu S.,Shanghai University | And 8 more authors.
Human Molecular Genetics | Year: 2015

Recently, a large number of long non-coding RNAs (lncRNAs) have been reported in mammalian genomes and are evolutionarily conserved and presumably function in many biological events, especially in the pathogenesis of diverse human cancers. A lncRNA, named HOST2 (human ovarian cancer-specific transcript 2), was once reported to specifically be expressed at high level in human ovarian cancer. However, how HOST2 acts to regulate gene functions in ovarian carcinogenesis has remained enigmatic. Here we report, for the first time, that HOST2 promotes tumor cell migration, invasion and proliferation in epithelial ovarian cancer by working in key aspects of biological behaviors. In the present study, bioinformatics analysis indicated that HOST2 binds with microRNA let-7b, a potent tumor suppressor, which was then verified to target HOST2. Our results showed that HOST2 harbors a let-7b binding site and modulates let-7b availability by acting as a molecular sponge. HOST2 inhibits let-7b functions, which post-transcriptionally suppress the expression of targets, including some oncogenes that regulate cell growth and motility. Additionally, understanding HOST2/let-7b-dependent regulation may lead to alternative approaches for the diagnosis and cure of this deadly disease. © The Author 2014. Published by Oxford University Press. All rights reserved.

Wei J.,Air Force General Hospital
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2012

To explore the effects of social support and personality traits on psychological characteristic of patients with chronic cervicodynia and lumbodynia and improve the level of diagnosis and treatment. From August 2009 to April 2010, 231 patients (obtained 217 effective responses) with chronic cervicodynia and lumbodynia were recruited. Among the patients, there were 123 males and 94 females, with an average age of (38.00 +/- 5.67) years (ranged from 15 to 66 years). Social Support Rating Scale (SSRS), Cattell Sixteen Personality Factor Questionnaire (16PF) and Symptom Checklist 90 (SCL-90) were used to test social support and psychological characteristic and compared the difference of psychological, personality traits and norm, then analyzed the effect of social support and personality traits on psychological characteristic. Two hundred and seventeen (93.9%) patients completed the questionnaire. Compared with normal 16PF scores, there were significant differences in factor scores of intelligence, stability, excitability, perseverance,social boldness, vigilance, sophistication, experimental, independence and tonicity (P < 0.01). And for SCL-90, the score of somatization, depression, anxiety, rivalrounsness, horror, bigotry, total score and mean score were higher than norm (P < 0.01). For SSRS, subjective support points and total points had positive effects; While intelligence, stability, perseverance, self-discipline had positive effects, vigilance and anxiety had negative effects. There were significant differences in personality traits and psychological characteristic between patients with chronic cervicodynia and lumbodynia and norms. Improving social support level and optimizing personality traits can improve psychological profile of these patients.

Ou W.-B.,Air Force General Hospital
Medical Science Monitor | Year: 2015

Background: The objective of this study was to perform a systematic review of correlations between the single-nucleotide polymorphism at nucleotide 309 (single-nucleotide polymorphism, SNP309) in the murine double-minute 2 (MDM2) gene promoter and susceptibility to leukemia.Material/Methods: We performed a computer search of relevant case-control studies published from January 1990 to Jan 2014 in databases such as Ovid, EBSCO, PubMed, CNKI, CBMDISC, VIP, and WanFang Data. The literature was screened based on inclusion and exclusion criteria. The data were retrieved, and the quality of the methodology used in the studies was evaluated. A meta-analysis was performed by calculating the combined odds ratios (OR) and 95% confidence intervals (CI) using RevMan 5.0 and Stata 10.0 software. Sensitivity was analyzed and publication bias was assessed.Results: A total of ten case-control studies from nine research papers were selected in this study, which included 1889 cases and 5707 controls. Meta-analysis showed that people who carried the G allele had increased susceptibility to leukemia compared to people who carried the T allele [OR=1.24, 95% CI (1.06, 1.45), P=0.007]. In a recessive model, the GG homozygotic population had a higher risk of leukemia than the heterozygotic GT+TT population [OR=1.47, 95% CI (1.11, 1.96), P=0.008]. We did not find significant difference in a dominant model [GG+GT vs. TT: OR=1.22, 95% CI (0.98, 1.52), P=0.07]. Publication bias was not significant.Conclusions: SNP309 polymorphism in the MDM2 gene is associated with susceptibility to leukemia. The G allele may be a risk factor for leukemia. © Med Sci Monit, 2015.

Wu W.,Air Force General Hospital
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation | Year: 2013

Fourteen patients with pancreatic carcinoma were selected. Two treatment plans were designed for each patient, including gamma knife and Tomotherapy. The dose characteristics were evaluated by DVH and were compared. The results showed that the gamma knife plan had the higher maximal and mean target dose than Tomotherapy. Body gamma knife can increase the target dose significantly, and decrease the OAR dose. Tomotherapy had excellent dose-target conformality, and it can control doses of duodenum and stomach easily, but it had larger low dose region.

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