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Song K.-Y.,Ahram Biosystems Inc. | Song K.-Y.,Kyung Hee University | Hwang H.J.,Ahram Biosystems Inc. | Kim J.H.,Kyung Hee University
Food Chemistry | Year: 2017

The aim of this study was to develop an ultra-fast molecular detection method for meat identification using convection Palm polymerase chain reaction (PCR). The mitochondrial cytochrome b (Cyt b) gene was used as a target gene. Amplicon size was designed to be different for beef, lamb, and pork. When these primer sets were used, each species-specific set specifically detected the target meat species in singleplex and multiplex modes in a 24 min PCR run. The detection limit was 1 pg of DNA for each meat species. The convection PCR method could detect as low as 1% of meat adulteration. The stability of the assay was confirmed using thermal processed meats. We also showed that direct PCR can be successfully performed with mixed meats and food samples. These results suggest that the developed assay may be useful in the authentication of meats and meat products in laboratory and rapid on-site applications. © 2017 Elsevier Ltd


Patent
Ahram Biosystems Inc. | Date: 2012-07-02

Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a users hand for use as a portable, simple to operate, and low cost PCR amplification device.


Patent
Ahram Biosystems Inc. | Date: 2012-07-02

Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a users hand for use as a portable, simple to operate, and low cost PCR amplification device.


The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.


Trademark
Ahram Biosystems Inc. | Date: 2011-06-21

Dropping pipettes for medical purposes; cartridges, and capillaries, namely, capillary tubes for samples for the purpose of medical or diagnostic analyses for diseases.


Trademark
Ahram Biosystems Inc. | Date: 2016-08-18

Scientific apparatus and instruments and laboratory apparatus for research purposes, namely, polymerase chain reaction thermocyclers used to amplify DNA or RNA sequences for analytical and research purposes.


Trademark
Ahram Biosystems Inc. | Date: 2012-01-17

Scientific reagents, namely, nucleic acid polymerases and mixtures comprising polymerase used in life science research or diagnostic applications including polymerase chain reaction (PCR); scientific reagent kits used for nucleic acid amplification, or nucleic acid amplification and detection comprising nucleic acid polymerase and buffer solution.


Trademark
Ahram Biosystems Inc. | Date: 2011-06-21

Reagents and preparations for use in life science research, namely, [ amino acids, ] nucleic acids, [ recombinant genes, ] recombinant proteins, [ gene vectors, transformed cells, and cell lines; ] enzymes for life science research; [ anti-aging agents for use in the manufacture of cosmetics; ] chemical reagents other than for medical purposes; organic acids, namely, organic acid salts for use in life science research; [ phosphatides; sulphides; germination inhibitors as anti-sprouting agents; plant growth regulators for agricultural use; ] chemical reagents and chemical reagent kits for biotechnology research comprised of DNA primers, DNA polymerase, and buffers [ biochemical and diagnostic reagents for protein detection; reagent kits for protein detection comprised of reporter genes, reporter proteins, and reporter molecules; biochemical and diagnostic reagents for protein separation; and reagent kits for protein separation comprised of affinity columns and buffers ].


Trademark
Ahram Biosystems Inc. | Date: 2011-07-26

Scientific apparatus and instruments and laboratory apparatus for research purposes, namely, [ glassware for scientific experiments in laboratories, spectroscopes, polarizing microscopes, spectrograph apparatus, ] DNA sequence amplification devices, namely, DNA-amplifiers, [ detection devices using fluorescence proteins, namely, fluorescence microscopes, ] detection devices using a gene, namely, fluorescence detection devices using dyes that bind or are bound to nucleic acids or genes for measuring fluorescence, nucleic acid sequence amplification devices, namely, DNA- or RNA-amplifiers, thermocyclers, polymerase chain reaction laboratory instruments, real time polymerase chain reaction instruments, namely, thermocyclers for the purpose of performing polymerase chain reactions, [ dispensers, namely, disposable dispenser syringes or pipettes for laboratory use; ] disposable articles for laboratory instruments, namely, incubators or reaction vessels for scientific reactions or analyses.


The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

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