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Seoul, South Korea

Lim G.,Kyung Hee University | Hwang H.J.,Ahram Biosystems Inc. | Kim J.H.,Kyung Hee University
Journal of Nanoscience and Nanotechnology | Year: 2014

Method of oriented and activity-preserved immobilization of biologically active proteins based on concepts of active-site masking and kinetic control was previously reported. We extended our study and found that the surface hydrophobicity had a noticeable effect on the activity of active-site protected immobilized (PIM) Taq DNA polymerase on mixed self-assembled monolayer (SAM) manufactured on Au surface. Hydrophobic SAM was created by using 12-mercaptododecanoic acid and 1-heptanethiol. The resulting Taq DNA polymerase activity was measured by performing PCR amplification and compared with previously reported values acquired with hydrophilic SAM. The maximum activity of immobilized Taq DNA polymerase was achieved at 17.5% of 12-mercaptododecanoic acid and within 90 min of reaction time which are higher than those acquired with hydrophilic SAM; maximum activity at 5% of 12-mercaptododecanoic acid and at 10 min. In order to be applicable to commercial level, immobilized enzyme need to be stable, reusable and storable. PIM Taq DNA polymerase was stably attached to the surface and could be used for as many as 10 PCR runs which were comparable to solution-phase enzyme. Even after 56 days of storage at 4 °C, immobilized enzyme pertained 70% of the initial PIM enzyme activity. These data suggest that PIM Taq DNA polymerase can be used for various commercial applications. Copyright © 2014 American Scientific Publishers.

Ahram Biosystems Inc. | Date: 2012-07-02

Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a users hand for use as a portable, simple to operate, and low cost PCR amplification device.

Ahram Biosystems Inc. | Date: 2011-06-21

Reagents and preparations for use in life science research, namely, amino acids, nucleic acids, recombinant genes, recombinant proteins, gene vectors, transformed cells, and cell lines; enzymes for life science research; anti-aging agents for use in the manufacture of cosmetics; chemical reagents other than for medical purposes; organic acids, namely, organic acid salts for use in life science research; phosphatides; sulphides; germination inhibitors as anti-sprouting agents; plant growth regulators for agricultural use; chemical reagents and chemical reagent kits for biotechnology research comprised of DNA primers, DNA polymerase, and buffers; biochemical and diagnostic reagents for protein detection; reagent kits for protein detection comprised of reporter genes, reporter proteins, and reporter molecules; biochemical and diagnostic reagents for protein separation; and reagent kits for protein separation comprised of affinity columns and buffers.

Ahram Biosystems Inc. | Date: 2011-06-21

Dropping pipettes for medical purposes; cartridges, and capillaries, namely, capillary tubes for samples for the purpose of medical or diagnostic analyses for diseases.

Ahram Biosystems Inc. | Date: 2010-07-20

Scientific apparatus and instruments and laboratory apparatus for research purposes, namely, polymerase chain reaction thermocyclers used to amplify DNA or RNA sequences for analytical and research purposes.

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