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Waukesha, WI, United States

Li G.,U.S. Department of Agriculture | Li G.,Northeast Agricultural University | Lillehoj H.S.,U.S. Department of Agriculture | Lee K.W.,U.S. Department of Agriculture | And 8 more authors.
Avian Pathology | Year: 2010

Gangrenous dermatitis (GD) is an emerging disease of increasing economic importance in poultry resulting from infection by Clostridium septicum and Clostridium perfringens type A. Lack of a reproducible disease model has been a major obstacle in understanding the immunopathology of GD. To gain better understanding of host-pathogen interactions in GD infection, we evaluated various immune parameters in two groups of birds from a recent commercial outbreak of GD, the first showing typical disease signs and pathological lesions (GD-like birds) and the second lacking clinical signs (GD-free birds). Our results revealed that GD-like birds showed: reduced T-cell and B-cell mitogen-stimulated lymphoproliferation; higher levels of serum nitric oxide and α-1-acid glycoprotein; greater numbers of K55+, K1+, CD8+, and MHC class II+ intradermal lymphocytes, and increased K55+, K1+, CD8+, TCR1+, TCR2+, Bu1+, and MHC class II+ intestinal intraepithelial lymphocytes; and increased levels of mRNAs encoding proinflammatory cytokines and chemokines in skin compared with GD-free chickens. These results provide the first evidence of altered systemic and local (skin and intestine) immune responses in GD pathogenesis in chickens. © 2010 Houghton Trust Ltd. Source

Yoon S.C.,U.S. Department of Agriculture | Lawrence K.C.,U.S. Department of Agriculture | Line J.E.,U.S. Department of Agriculture | Siragusa G.R.,Agtech Products Inc. | And 3 more authors.
Sensing and Instrumentation for Food Quality and Safety | Year: 2010

The presence of Campylobacter in foods of animal origin is the leading cause of bacterially induced human gastroenteritis. Isolation and detection of Campylobacter in foods via direct plating involves lengthy laboratory procedures including enrichments and microaerobic incubations, which take several days to a week. The incubation time for growing Campylobacter colonies in agar media usually takes 24-48 h. Oftentimes the problem is the difficulty of visually differentiating Campylobacter colonies from non-Campylobacter contaminants that frequently grow together with Campylobacter on many existing agars. In this study, a new screening technique using non-destructive and non-contact hyperspectral imaging was developed to detect Campylobacter colonies in Petri dishes. A reflectance spectral library of Campylobacter and non-Campylobacter contaminants was constructed for characterization of absorption features in wavelengths from 400 to 900 nm and for developing classification methods. Blood agar and Campy-Cefex agar were used as culture media. The study found that blood agar was the better culture medium than Campy-Cefex agar in terms of Campylobacter detection accuracy. Classification algorithms including single-band thresholding, band-ratio thresholding and spectral feature fitting were developed for detection of Campylobacter colonies as early as 24 h of incubation time. A band ratio algorithm using two bands at 426 and 458 nm chosen from continuum-removed spectra of the blood agar bacterial cultures achieved 97-99% of detection accuracy. This research has profound implications for early detection of Campylobacter colonies with high accuracy. Also, the developed hyperspectral reflectance imaging protocol is applicable to other pathogen detection studies. © 2010 US Government. Source


Agtech Products Inc. | Date: 2009-04-16

Animal feed additive for use as a nutritional supplement; antimicrobial preparations for controlling spoilage or reducing bacterial pathogens by inhibiting microbiological decomposition.

Agtech Products Inc. | Date: 2008-05-13

animal feed additive for use as a nutritional supplement.


Agtech Products Inc. | Date: 2008-04-29

Animal feed additive for use as a nutritional supplement.

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