Kekina H.G.,Agrochemical Research Center |
Golubkina N.A.,Agrochemical Research Center |
Tulchinskaya O.V.,Childrens Policlinic of Vlasikha
Voprosy Pitaniia | Year: 2014
Fish is known to be a significant source of iodine and selenium for human beings. The aim of the present work was evaluation of iodine and selenium consumption levels with fish by residents of Moscow Region and Moscow. 400 Residents of Moscow and Moscow Region (100 children of 2-6 years age, 100 adults of 20-35 years age, 100 students of 18-22 years age and 100 elderly persons of 50-75 years age were inspected using values of ioduria and Se status determination. I concentration was determined by voltamperometric method, Se - via semiquantitative peroxide test. The values of ioduria for the inhabitants corresponded to moderate (Moscow Region, ioduria median 52,5μg/l) and light (Moscow, ioduria mediane 67 μg/l) I deficiency with marginal Se deficiency in both cases (79-90% of persons had a negative peroxide test parameters, corresponding to serum Se level >90 μg/l). Though main fish species used by the population (humpback, trout, steelhead) contain relatively high levels of Se (505±46, 376±32, 413±22 pg/kg) and I (187±66, 290±102, 330±116 μg/kg), they are not able to maintain high I and Se status of the inhabitants due to low consumption level. I consumption with fish, being used once per week, reached 21 μg, Se - 35 μg per week. Up to 40% of students and 28% of elderly do not eat fish at all. Children of 2-6 years old residing in Moscow Region compose a special group of ecological risk of Ideficiency possessing significant I deficiency 3 times more frequently than children from Moscow.
Nakao T.,Agrochemical Research Center |
Naoi A.,Agrochemical Research Center |
Kawahara N.,Agrochemical Research Center |
Hirase K.,Agrochemical Research Center
Pesticide Biochemistry and Physiology | Year: 2010
We found the A2'N mutation (index number for M2 membrane spanning region) in the GABA receptor subunit of fipronil-resistant Sogatella furcifera, by analyzing DNA sequences amplified from fipronil-resistant and -susceptible S. furcifera. In order to confirm the role of A2′N mutation in the fipronil resistance, we expressed the wild-type and A2′N mutant Drosophila GABA receptors in Drosophila Mel-2 cells stably. Amino acid sequences of three membrane spanning regions (M1-M3), which are important for binding of fipronil, are conserved between Drosophila and S. furcifera. So the results of A2′N mutant Drosophila GABA receptor suggest the role of A2′N mutation in fipronil-resistant S. furcifera. The membrane potential assay showed that the A2′N mutant Drosophila GABA receptor was not inhibited by fipronil at all, while the IC50 value of fipronil for wild-type Drosophila GABA receptor was 172nM. These results suggest that A2′N mutation confers the resistance of fipronil in S. furcifera. © 2010 Elsevier Inc.