Šumperk, Czech Republic


Šumperk, Czech Republic
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Flores F.,University of Huelva | Hybl M.,AGRITEC Ltd. | Knudsen J.C.,Toft Plant Breeding | Marget P.,French National Institute for Agricultural Research | And 11 more authors.
Field Crops Research | Year: 2013

Faba bean (Vicia faba L.) is a cool season grain legume whose acreage has constantly declined in traditional producer countries as it has been replaced by more productive cereal crops. However, faba bean is still considered to have great potential as rainfed crop. In order to satisfy the renewed interest in faba bean cultivation yield stability should be improved by exploiting different germplasm types and sowing seasons.In order to understand of genotype by environment interactions and to compare cultivar performance over years and locations a spring faba bean network was established with 20 cultivars grown over three crop seasons at 13 contrasting locations covering most of Europe. Analysis was performed by heritability-adjusted genotype plus genotype × environment interaction (HA-GGE) biplot analysis. HA-GGE biplot analyses identified that the effect of genotype by environment interaction was higher than the effect of genotypes, allowing identification of three mega-environments, namely Continental, Oceanic, and Mediterranean, in which cultivar performed differently. This supports the need for specific breeding for each specific geoclimatic area. Espresso was the highest yielding cultivar, being also highly stable over the Oceanic and Continental mega-environments. Cultivars Fuego, Hobbit and SR-1060 had also good yield but with a moderate unstability in both Oceanic and Continental mega-environments. Baraca and Alameda yielded poorly at all environments although were the best yielders at Mediterranean locations. Environments as Sumperk and Premesques were identified as the best core test locations for Continental and Oceanic mega-environments, respectively, being the locations in which best genotypes could be most easily identified. © 2013 Elsevier B.V.

Rubiales D.,CSIC - Institute for Sustainable Agriculture | Avila C.M.,IFAPA Centro Alameda del Obispo | Sillero J.C.,IFAPA Centro Alameda del Obispo | Hybl M.,AGRITEC Ltd. | And 3 more authors.
Field Crops Research | Year: 2012

Ascochyta blight incited by Ascochyta fabae is a widespread disease severely damaging faba bean (Vicia faba) production. Insufficient resistance is available in faba bean cultivars. In an attempt to identify new sources of resistance a germplasm collection of 484 V. faba accessions was screened for resistance to A. fabae under field conditions; stability of resistance of the 19 most resistant accessions was tested in a multi-location experiment in Czech Republic, Estonia, Germany and Spain over two field seasons. Although complete resistance was not found, six accessions showed interesting levels of incomplete resistance. Genotype. ×. environment (G. ×. E) interaction accounted for 42% of the sum of squares of the multi-environment evaluation, revealing instability of the phenotypic expression across environments. Accessions L-831818, V-26 and V-958 stand out for their consistent resistance, being the most resistant and stable across environments. Accessions V-255, V-1020, V-1085 and V-1117 displayed good resistance, but lower stability, with V-1020, V-1085 and V-1117 being more suitable for Córdoba (Mediterranean mega-environment), and V-255 more suitable for Hohenlieth, Jõgeva and Sumperk (Continental mega-environment). © 2011 Elsevier B.V.

Soudek P.,Czech Republic Crop Research Institute | Katrusakova A.,Czech Republic Crop Research Institute | Katrusakova A.,Institute of Chemical Technology Prague | Sedlacek L.,Czech Republic Crop Research Institute | And 6 more authors.
Archives of Environmental Contamination and Toxicology | Year: 2010

The effect of toxic metals on seed germination was studied in 23 cultivars of flax (Linum usitatissimum L.). Toxicity of cadmium, cobalt, copper, zinc, nickel, lead, chromium, and arsenic at five different concentrations (0.01-1 mM) was tested by standard ecotoxicity test. Root length was measured after 72 h of incubation. Elongation inhibition, EC50 value, slope, and NOEC values were calculated. Results were evaluated by principal component analysis, a multidimensional statistical method. The results showed that heavy-metal toxicity decreased in the following order: As3+ ≥ As5+ > Cu2+ > Cd2+ > Co2+ > Cr 6+ > Ni2+ > Pb2+ > Cr3+ > Zn2+. © 2010 Springer Science+Business Media, LLC.

Smykalova I.,AGRITEC Ltd | Horacek J.,AGRITEC Ltd | Kubosiova M.,Mendel University in Brno | Smirous Jr. P.,AGRITEC Ltd | And 4 more authors.
In Vitro Cellular and Developmental Biology - Plant | Year: 2012

Plant regeneration was obtained from cultured anthers and hypocotyl segments of caraway (Carum carvi L.). Microspore-and somatic tissue-derived embryos were compared by observation of the regeneration process under identical induction conditions. Fluorescent microscopy with DAPI staining showed initiation of cell divisions and formation of embryogenic callus and somatic embryos from anther sacs, with production of embryos of both microspore and somatic origin. Induction of somatic embryos from hypocotyl-derived callus was also demonstrated. Isozyme native polyacrylamide gel electrophoresis was used to identify haploids and doubled haploids, and to determine the frequency of spontaneous diploidization of regenerated plants of microspore origin. Donor plants (2n = 20) and their anther-derived derivative plants (n = 10, 2n = 20, 4n = 40) in callus stage or leafy rosette stage were compared. The esterase (EST) band patterns of regenerated plants differed from the heterozygous parental material, suggesting that the regenerated plants were microspore-derived haploid/doubled haploid plants. The similar profile of EST bands between the diploid anther-derived plants and a sample of the donor plants corresponded to a somatic regeneration pathway. Although the selected induction conditions revealed no preference for induction of microspore embryogenesis, the anther culture protocol established for caraway utilizing isozyme segregating EST loci markers is suitable for DH production. © 2011 The Society for In Vitro Biology.

Hradilova J.,Mendel University in Brno | Hradilova J.,Academy of Sciences of the Czech Republic | Rehulka P.,Academy of Sciences of the Czech Republic | Rehulkova H.,Academy of Sciences of the Czech Republic | And 4 more authors.
Electrophoresis | Year: 2010

Cadmium (Cd) is classified as a serious pollutant due to its high toxicity, high carcinogenicity, and widespread presence in the environment. Phytoremediation represents an effective low-cost approach for removing pollutants from contaminated soils, and a crop with significant phytoremediation potential is flax. However, significant differences in Cd accumulation and tolerance were previously found among commercial flax cultivars. Notably, cv. Jitka showed substantially higher tolerance to elevated Cd levels in soil and plant tissues than cv. Tábor. Here, significant changes in the expression of 14 proteins (related to disease/defense, metabolism, protein destination and storage, signal transduction, energy and cell structure) were detected by image and mass spectrometric analysis of two-dimensionally separated proteins extracted from Cd-treated cell suspension cultures derived from these contrasting cultivars. Further, two proteins, ferritin and glutamine synthetase (a key enzyme in glutathione biosynthesis), were only up-regulated by Cd in cv. Jitka, indicating that Cd tolerance mechanisms in this cultivar may include maintenance of low Cd levels at sensitive sites by ferritin and low-molecular weight thiol peptides binding Cd. The identified changes could facilitate marker-assisted breeding for Cd tolerance and the development of transgenic flax lines with enhanced Cd tolerance and accumulation capacities for phytoremediating Cd-contaminated soils. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

Smykalova I.,AGRITEC Ltd. | Vrbova M.,AGRITEC Ltd. | Tejklova E.,AGRITEC Ltd. | Vetrovcova M.,AGRITEC Ltd. | Griga M.,AGRITEC Ltd.
Industrial Crops and Products | Year: 2010

The in vitro shoot-tip culture obtained from hypocotyl segments was established for screening of genetically based ability to accumulate and/or tolerate metals, Cd and Zn, in a set of commercial flax/linseed (Linum usitatissimum L.) varieties grown in the Czech Republic. Callus-induction and organogenesis in explants (bud and root regeneration, shoot development) at increasing concentrations of Cd and Zn in the cultivation medium were evaluated. Sub-lethal Cd and Zn levels (19mg/l Cd2+ and 33mg/l Zn2+) were established for in vitro selection system to find out flax/linseed lines with improved Cd/Zn accumulation and/or Cd/Zn tolerance. Metal accumulation in regenerated shoots, the most important parameter for the assessment of variety accumulation capacity, was influenced by regeneration ability of explants in individual varieties. Group of tolerant varieties was selected using established in vitro method. Two different mechanisms of Cd or Zn tolerance were found out. The selected flax/linseed varieties with the highest metal accumulation capacity can be used for phytoremediation purposes immediately. They also can be employed in the agrobacterial transformation experiments for additional metal accumulation increasing. Varieties with the highest Zn accumulation can be used in next studies with the aim to select variety with the high Zn accumulation in seeds which can be utilized in the case of the Zn deficiency in human diet. © 2010 Elsevier B.V.

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