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Saskatoon, Canada

The narrow genetic base in spring Brassica napus (AACC) canola is a limitation for continued improvement of this crop. This research focused on broadening of genetic diversity in spring canola by using B. oleracea (CC). Seeds of B. oleracea contain high levels of erucic acid and glucosinolates, which are undesired in canola. Therefore, inheritance of these traits and the prospect of developing spring canola with allelic diversity introgressed from B. oleracea were investigated in B. napus Х B. oleracea interspecific progenies. Zero-erucic plants in F2 generation occurred at a lower frequency than expected based on segregation involving only the C-genome erucic acid alleles. Selection in F2 to F3 focused on zero erucic acid, while focus in later generation was for low glucosinolate and B. napus plants. In the F6, 31% zero erucic families had low glucosinolate content. Flow cytometry analysis of the F8 families showed no significant difference from the B. napus parent. Genetic diversity analysis by using simple sequence repeat markers from the C-genome chromosomes showed that the F8 families received up to 54% alleles from B. oleracea. The results demonstrate the feasibility of enriching genetic diversity in B. napus canola by using B. oleracea. © 2015, Agricultural Institute of Canada. All Rights Reserved. Source

Whittle C.A.,National Research Council Canada | Malik M.R.,National Research Council Canada | Malik M.R.,Agrisoma Biosciences Inc. | Li R.,National Research Council Canada | Krochko J.E.,National Research Council Canada
Plant Molecular Biology | Year: 2010

Transcriptome data for plant reproductive organs/cells currently is very limited as compared to sporophytic tissues. Here, we constructed cDNA libraries and obtained ESTs for Brassica napus pollen (4,864 ESTs), microspores (i.e., early stage pollen development; 6,539 ESTs) and ovules (10,468 ESTs). Clustering and assembly of the 21,871 ESTs yielded a total of 10,782 unigenes, with 3,362 contigs and 7,420 singletons. The pollen transcriptome contained high levels of polygalacturonases and pectinesterases, which are involved in cell wall synthesis and expansion, and very few transcription factors or transcripts related to protein synthesis. The set of genes expressed in mature pollen showed little overlap with genes expressed in ovules or in microspores, suggesting in the latter case that a marked differentiation had occurred from the early microspore stages through to pollen development. Remarkably, the microspores and ovules exhibited a high number of co-expressed genes (N = 1,283) and very similar EST functional profiles, including high transcript numbers for transcriptional and translational processing genes, protein modification genes and unannotated genes. In addition, examination of expression values for genes co-expressed among microspores and ovules revealed a highly statistically significant correlation among these two tissues (R = 0.360, P = 1.2 × 10 -40) as well as a lack of differentially expressed genes. Overall, the results provide new insights into the transcriptional profile of rarely studied ovules, the transcript changes during pollen development, transcriptional regulation of pollen tube growth and germination, and describe the parallels in the transcript populations of microspore and ovules which could have implications for understanding the molecular foundation of microspore totipotency in B. napus. © Her Majesty the Queen in Right of Canada 2009. Source

The present invention relates to transgenic plants comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acid comprising a coding sequence operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant, said nucleic acid being stably integrated at or adjacent to rDNA sequences, or a seed, organ, tissue, part or cell thereof, or a descendant of said plant, seed, organ, tissue, part or cell; methods of producing the transgenic plants; and methods of producing oil using the transgenic plants.

Agrisoma Biosciences Inc. | Date: 2011-12-20

(Based on Intent to Use) Agricultural seeds(Based on 44(d) Priority Application) Agricultural seeds.

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