Rapotín, Czech Republic
Rapotín, Czech Republic

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Riha J.,Agriresearch Rapotin Ltd | Riha J.,R.Ø.S.A. | Bezdicek J.,Palacky University
Bulgarian Journal of Agricultural Science | Year: 2016

The aim of this study was to assess the effect of the relative breeding value of the paternal growth of sires on highly valued parts of carcasses in their progeny. The study was carried out on nine Beef breeds in the Czech Republic. Cutting parts evaluated were: round, strip loin and tenderloin (First class meat); rib, shoulder blade (boneless), fore shank, flank, chuck roll + neck (Second-class meat) and separable fat. For statistical data analysis was used STATISTICA 8 software (Statistica 8, StatSoft Inc., 2008. Tulsa, OK). The data were processed using canonical analysis to evaluate basic relationships in the two groups of selected parameters. The group on the right set consisted of selected cuts. The group on the left set consisted of relative breeding values of paternal growth effect, age, weight and breed. Correlation analysis of relative breeding value revealed a significant relationship only to separable fat, flank and strip loin. Although this correlation was significant, their value was low. For the other breeding traits there were no significant correlations with different cutting parts. Based on the results of canonical analysis, 10.42% explained variability in the context of the controlled variables of the left set of parameters (breed, RBVseg, age and weight) can be explained using single cuts. We also found that only 5.95% of the explained variability of meat cuts – variables of the right set of parameters can be explained using breed, RBVseg, age and weight. © 2016, National Centre for Agrarian Sciences. All rights reserved.


Krizova L.,Agriresearch Rapotin Ltd. | Watzkova J.,Agriresearch Rapotin Ltd. | Trinacty J.,Agriresearch Rapotin Ltd. | Richter M.,Agriresearch Rapotin Ltd. | Buchta M.,R.Ø.S.A.
Czech Journal of Animal Science | Year: 2011

The objective of this study was to determine rumen degradability and total digestibility of flavonolignans from a milk thistle fruit expeller in dairy cows considering milk production and changes in plasma flavonolignans. The experiment was carried out on three lactating Holstein cows and was divided into three periods as follows: preliminary period (Pr, 3 days) was used for the diet stabilization followed by the adaptation period (A, 6 days) in which the treatment was applied and by the balance period (B, 4 days). Cows were fed individually twice a day (6:40 and 16:40 h) ad libitum the diet based on maize silage, lucerne hay and supplemental mixture. In the periods A and B the diet was supplemented with 150 g/day of milk thistle fruit expeller applied in two equal portions at each feeding. Average daily intake of dry matter and basic nutrients was similar in all periods (P gt; 0.05). Milk yield and composition were not affected by the treatment (P gt; 0.05). The milk thistle fruit expeller used in this experiment contained 4.10 ± 0.10 mass percentage of the silymarin complex. Digestibility of silybin A and silybin B was 40.0 and 45.5%, respectively. Digestibility of other components of the silymarin complex was 100%. The highest value of the effective degradation was found for taxifolin (59.11%), while the effective degradation of the other flavonolignans ranged from 23.28 to 35.19%. Animals receiving the milk thistle fruit expeller had a higher content of plasma conjugated silybin (P <0.001) than those without its supplementation.


Manga I.,Agriresearch Rapotin Ltd. | Manga I.,Research Institute for Cattle Breeding Ltd. | Riha H.,Research Institute for Cattle Breeding Ltd.
Archiv fur Tierzucht | Year: 2011

The present study investigated the K232A mutation of the diacylglycerol O-acyltransferase 1 gene (DGAT1) in 315 Czech Holstein Cows. The allele frequency was found to be 0.19 for the K allele and 0.81 for the A allele. The results of K232A testing were assessed in relation to average daily milk yield (l), percentage of fat, protein, lactose and milk somatic cell count (SCC, thousand/ml). A GLM procedure was used to analyse the differences among genotypes. The K232A genotypes were significantly associated with milk fat percentage (KK, KA>AA [P≤0.05, P≤0.005]) and milk yield (KK, KA>AA [P≤0.05, P≤0.005]). The K allele was also favourable for SCC levels: Cows with the KA genotype had lower SCC levels than those with the AA genotype (P≤0.05), while cows with the KK genotype showed the lowest levels of SCC at all. This new association of K232A suggests the existence of another gene in the centromeric region on BTA14 linked to DGAT1 with direct effect on the SCC. On the basis of a broad range of DGAT1 protein functions and the non-conservative matter of K232A, a direct effect of K232A on the SCC cannot be ruled out either. © Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.


Manga I.,Agriresearch Rapotin Ltd. | Dvorak J.,Mendel University in Brno
Czech Journal of Animal Science | Year: 2010

Alleles A1 and A2 of the Bos taurus CSN2 gene are the most frequent in a number of dairy cattle breeds. In this study, a new allelic discrimination assay using TaqMan fluorogenic probes was developed to detect single nucleotide substitution characterizing the A1/A2 alleles of the CSN2 gene. The method was validated using DNA samples of known genotypes with different concentrations and the results were compared with those for the commonly used problematic ACRS-PCR. We found the TaqMan method to be more effective, 100% reliable and hundred times more sensitive for testing the CSN2 genetic marker in cattle than the ACRS-PCR. As it enabled a rapid analysis of a large number of DNA samples in uniform format without previous DNA quality assessment and without the requirement for post-amplification manipulations, it presents an effective tool for the analysis of large-scale sample sets. The method was applied for testing on a sample of 120 Czech Holstein dairy cows. The observed relative genotype and allele frequencies were as follows: A1A1-0.20, A1A2-0.51, A2A2-0.29; A1-045, A2-0.55.


Bezdicek J.,Palacky University | Stadnik L.,Czech University of Life Sciences | Louda F.,Agriresearch Rapotin Ltd
Bulgarian Journal of Agricultural Science | Year: 2015

The aim of this study was to evaluate the effect of inbreeding depression on age at first calving in Holstein and Czech Fleckvieh cows. Only cows with a complete pedigree to the fifth generation were used. Data were collected from 34 farms in the Czech Republic. For proper comparison, each inbred cow (n=1046) was assigned to at least one noninbred equal (n=1046). Inbred and noninbred pairs were matched on characteristics such as identical father, first calving interval occurring at the same farm and calves born in the same year and month (± 2 months). The PROC GLM of SAS® with five fixed effects was applied to all data. Inbred Holstein and Czech Fleckvieh cows and their matched noninbred equals were then divided according to inbreeding coefficient into four (event. five) groups. For Holstein inbred groups: FX=1.25; FX=3.125; FX=6.0-12.0; FX=25.0; FX>25; respectively all animals, the following extension of age at first calving was found: +2.7; +6.9; +9.5; +18.5; +26.8; +9.6 days, respectively. While for Czech Fleckvieh inbred groups: FX=1.25; FX=3.125; FX=6.0-12.0; FX=25.0; respectively all animals, the prolongation of age at first calving was detected on the level +2.3; +3.9; +9.8; +21.3; +9.8 days, respectively. This study showed the negative effect of inbreeding on prolongation of age at first calving. Deterioration in this reproduction trait occurred even at low levels of the coefficient FX. At higher levels the differences between inbred and noninbred cows were significant (P<0.05-0.01). © 2015, National Centre for Agrarian Sciences. All rights reserved.


Richter M.,Agriresearch Rapotin Ltd. | Krizova L.,Agriresearch Rapotin Ltd. | Trinacty J.,Agriresearch Rapotin Ltd.
Czech Journal of Animal Science | Year: 2010

The aim of this study was to continuously monitor ruminai pH and redox potential of individual dry cows using a newly developed wireless device. Three dry Holstein cows fitted with rumen cannulas were used for the individual measurement of ruminal pH and redox potential (Eh) using a newly developed wireless device. The experiment was carried out in the period of 14 days consisting of a 10-day preliminary period followed by a 4-day measurement period. Cows were fed twice daily the diet based on maize silage, lucerne hay and concentrate. During the measurement period ruminal pH and redox potential were monitored continuously using a developed wireless probe. Average daily feed intake throughout the experiment was 8.2 kg/day. The mean ruminai pH was almost identical in Cows 21 and 25, being 6.79 and 6.75, respectively, and was lower than in Cow 26 (6.86; P < 0.05). The mean Eh of the ruminai fluid was -274 mV in Cow 21 and 26 and -270 mV in Cow 25, while the results did not differ significantly (P > 0.05). The diurnal pattern of ruminai pH and Eh showed a similar trend in all animals. Mean values of rH (Clark's exponent) calculated for Cows 21 and 25 being 4.43 and 4.48, respectively, were lower than the value calculated for Cow 26 (4.59; P < 0.05). This method may be useful for investigating factors affecting the dynamics of ruminai fermentation and may also help in the identification of variables associated with various metabolic disorders.


Trinacty J.,Agriresearch Rapotin Ltd. | Richter M.,Agriresearch Rapotin Ltd. | Krizova L.,Agriresearch Rapotin Ltd.
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2016

The trial was carried out on four Holstein cows with initial milk yield of 27.3 ± 1.7 kg.day-1. Cows were divided into two groups - The first was fed a diet based on extruded rapeseed cake (D-ERC), the second one was fed a diet based on extruded full-fat soybean (D-EFFS), both diets contained maize silage and meadow hay. The experiment was divided into 4 periods of 42 days. Intake of dry matter, crude protein and NEL was not affected by the treatment (P > 0.05) while the intake of PDIA, PDIN and PDIE was lower in D-ERC than in D-EFFS (P<0.05). Milk yield in D-ERC (22.6 kg.d-1) was lower than in D-EFFS (24.7 kg.d-1, P < 0.001) while concentration of milk fat and protein were reverse (P < 0.05). Smaller portion of essential AADI in crude protein intake (CPI) in D-ERC resulted in lower efficiency of CPI utilization for milk protein synthesis in comparison to D-EFFS being 313 and 327 g.kg-1, respectively (P < 0.01). Concentration of AA in blood plasma was not affected by the type of diet except of His and Ile that were higher in D-EFFS (P < 0.01).


Beran J.,Czech University of Life Sciences | Stadnik L.,Czech University of Life Sciences | Bezdicek J.,Agriresearch Rapotin Ltd. | Louda F.,Czech University of Life Sciences | And 2 more authors.
Archiv fur Tierzucht | Year: 2012

The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk in extenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawed artificial insemination (AI) doses, and during thermodynamic testing compared to extenders not containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each of the 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMed and Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A total of 640 AI doses were analysed. The volume of samples, sperm concentration, and percentage of motile spermatozoa were evaluated after collection, as was sperm motility after thawing of AI doses and during thermodynamic testing. Percentages of live and dead sperm were also evaluated. The data set was analysed using SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA). The results confirmed significant (P<0.05-0.01) between-sire differences in the volume, density, and activity of sperm as well as in share of live and dead sperm after collection; in decline of sperm motility in the fresh ejaculate, after thawing, and during the entire thermodynamic test, as well as in the share of live and dead sperm after thawing. The extenders ranked by sperm motility are: Optidyl, Triladyl, AndroMed, and Bioxcell, demonstrating the higher quality of AI doses produced using egg yolk extenders. Differences in sperm motility were significant (P<0.05-0.01) during the entirety of thermodynamic testing. Egg yolk extenders had a significantly (P<0.05-0.01) higher percentages of live sperm after thawing. © Leibniz Institute for Farm Animal Biology.


Ghara M.,Indian Institute of Science | Ghara M.,Indian Agricultural Research Institute | Ranganathan Y.,Indian Institute of Science | Ranganathan Y.,Agriresearch Rapotin Ltd. | And 4 more authors.
Arthropod-Plant Interactions | Year: 2014

Differential occupancy of space can lead to species coexistence. The fig-fig wasp pollination system hosts species-specific pollinating and parasitic wasps that develop within galls in a nursery comprising a closed inflorescence, the syconium. This microcosm affords excellent opportunities for investigating spatial partitioning since it harbours a closed community in which all wasp species are dependent on securing safe sites inside the syconium for their developing offspring while differing in life history, egg deposition strategies and oviposition times relative to nursery development. We determined ontogenetic changes in oviposition sites available to the seven-member fig wasp community of Ficus racemosa comprising pollinators, gallers and parasitoids. We used species distribution models (SDMs) for the first time at a microcosm scale to predict patterns of spatial occurrence of nursery occupants. SDMs gave high true-positive and low false-positive site occupancy rates for most occupants indicating species specificity in oviposition sites. The nursery microcosm itself changed with syconium development and sequential egg-laying by different wasp species. The number of sites occupied by offspring of the different wasp species was negatively related to the risk of syconium abortion by the plant host following oviposition. Since unpollinated syconia are usually aborted, parasitic wasps ovipositing into nurseries at the same time as the pollinator targeted many sites, suggesting response to lower risk of syconium abortion owing to reduced risk of pollination failure compared to those species ovipositing before pollination. Wasp life history and oviposition time relative to nursery development contributed to the co-existence of nursery occupants. © 2014 Springer Science+Business Media Dordrecht.


Krizova L.,Agriresearch Rapotin Ltd | Richter M.,Agriresearch Rapotin Ltd | Trinacty J.,Agriresearch Rapotin Ltd | Riha J.,Agriresearch Rapotin Ltd | Kumprechtova D.,Czech University of Life Sciences
Czech Journal of Animal Science | Year: 2011

An experiment was carried out on four dry Holstein cows fitted with rumen cannulas that were divided into two groups. The crossover design experiment was divided into 4 periods of 3 weeks. Each period consisted of a 17-day preliminary period followed by a 4-day experimental period. Cows were fed twice daily the total mixed ration based on maize silage and concentrate. Control cows (Control) received the basal diets while experimental animals (Yeast) received the basal diet supplemented with 3.0 g of live yeast (BIOSAF Sc 47, Lesaffre, France) at each feeding. During each experimental period ruminal pH and redox potential (Eh) were monitored continuously using a developed wireless probe. Further, in each experimental period five samples of ruminal fluid were taken at 6:30, 8:30, 10:30, 13:30 and 16:30 h to determine the content of volatile fatty acids, lactic acids and ammonia. On the last day of each period, blood samples were taken for determination of blood parameters and acid-base balance. Average daily dry matter intake throughout the experiment was 8.2 kg/day and was not affected by the treatment. The average ruminal pH in Control was 6.16 that was significantly lower than in Yeast, being 6.26 (P < 0.001). The diurnal pattern of ruminal pH showed a similar trend in both groups. Mean Eh in Control (-210 mV) differed significantly from Yeast (-223 mV, P < 0.001). The mean value of rH (Clark's Exponent) calculated for Control (5.33) was higher than that calculated for Yeast (5.09, P < 0.001). Total VFA concentrations were on average 40.8mM in Control and 57.2mM in Yeast (P > 0.05). Lactate and ammonia concentrations at individual sampling times and overall mean did not differ significantly between treatments (P > 0.05). Blood pH and C0 2 were not affected by the treatment.

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