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Pouralibaba H.R.,CSIC - Institute for Sustainable Agriculture | Pouralibaba H.R.,Dryland Agriculture Research Institute | Rubiales D.,CSIC - Institute for Sustainable Agriculture | Fondevilla S.,CSIC - Institute for Sustainable Agriculture
European Journal of Plant Pathology | Year: 2016

Fusarium oxysporum f.sp. lentis (Fol) is one of the most destructive diseases of lentil (Lens culinaris) worldwide. Although differences in in vitro growth pattern and aggressiveness have been observed in its populations, no pathotypes have been reported so far. The objective of this study was to check for the existence of differences in virulence in Fol and to develop a differential set of lentil accessions able to distinguish the virulence patterns. In a first step 28 lentil-resistant accessions reported elsewhere were inoculated with six isolates of Fol with different geographical origins, which showed a highly significant isolate × accession interaction. Based on their contrasting response, four accessions were selected as a putative differential set. In a second step we characterized the virulence pattern of 52 Fol isolates from Iran, Syria and Algeria using the differential set. This allowed the identification of seven different patterns of virulence, named as pathotypes 1 – 7. Pathotype 7 was virulent on all accessions studied. No correlation was found between the pathotype and the geographical origin of the isolates. This is the first report of the existence of pathotypes in Fol. Based on these results; lentil breeding strategies for resistance to the pathogen should be planned according to the prevalent pathotype present in the region. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging. Source


Pouralibaba H.R.,Dryland Agriculture Research Institute | Rubiales D.,CSIC - Institute for Sustainable Agriculture | Fondevilla S.,CSIC - Institute for Sustainable Agriculture
European Journal of Plant Pathology | Year: 2015

Vascular wilt caused by Fusarium oxysporum f.sp. lentis is a major disease of lentil (Lens culinaris) worldwide. In this study, 196 lentil landraces were screened for resistance under controlled and field conditions. In order to characterize different levels of incomplete resistance, a detailed method was developed to evaluate resistance to this pathogen under controlled conditions assessing symptoms in each leaf separately at weekly intervals and calculating Area under Disease Progress Curve (AUDPC). Consequently, selected accessions were screened in the field and the resistance confirmed. Twelve accession having good levels of resistance were identified. This article provides a new detailed method to evaluate incomplete resistance to the pathogen in lentil and identify new sources of resistance to the disease. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging. Source


Mohammadi M.,Dryland Agriculture Research Institute | Mohammadi M.,Islamic Azad University
African Journal of Biotechnology | Year: 2012

High temperature in terminal growth stages is a major stress of wheat productivity in South-west Iran, as well as in other Mediterranean environments and the long-term spring temperatures trend to increase. Determination of affecting factors or traits helps to improve the yield potential of wheat. The effects of heat stress during and post anthesis for physiologic, phenologic and agronomic traits was evaluated in ten bread wheat genotypes. The research was conducted under field condition in two different dates under less and more heated environments (two different sowing times). Also, source levels were manipulated through 50% spikelet removal at anthesis to evaluate cultivar source/sink limitations to kernel growth. The results depicted that grain yield, kernel number per spike and 1000 kernel weight were reduced by 24.1%, 9.2% and 23.7% in warmer environment, respectively. Hence, kernel weight was more suited for heat stress screening than other traits evaluated in this study. Thus, wheat genotypes that are able to maintain high individual kernel weight despite heat stress may possess a high level of heat tolerance. Furthermore, results indicated that the poor grain filling could mainly be attributed not to sink-limited conditions, but to source-limited conditions. © 2012 Academic Journals. Source


Mohammadi N.,Tarbiat Modares University | Puralibaba H.,Dryland Agriculture Research Institute | Goltapeh E.M.,Tarbiat Modares University | Ahari A.B.,University of Tabriz | Sardrood B.P.,Tarbiat Modares University
Phytoparasitica | Year: 2012

Fusarium oxysporum f. sp. lentis is the most important pathogen of lentil plants, and most areas under lentil cultivation are reported to have a fusarium wilt disease background. The plants are infected in the seedling stage and later stages of their development. Fusarium wilt disease, which has appeared at high incidence rates during recent years, has caused sharp drops in the yield, especially in Moghan, in the northwest of Iran. Forty-five isolates of the pathogen were collected from different regions of the country with two isolates from ICARDA in the summer of 2008 and identified using Nelson's key. The pathogenicity of the collected isolates was studied on a sensitive line (ILL 4605) under greenhouse conditions and significant differences in pathogenicity were found among them. The most pathogenic isolates from three provinces, East Azerbaijan (EA 30), Ardebil (Ar 3) and Khorasan (Kh 45), were selected and used in screening of 55 developed lines under greenhouse and field conditions. In the greenhouse, test plants were inoculated by immersing root tips in spore suspension and sowing seeds in pre-infested pot soil. Field tests were carried out in a naturally highly infested farm. At all stages, the plant response to the disease was based on the percentage of dead plants. Cluster analyses of the greenhouse and field data led to the selection of three lines (81S15, FLIP2007-42 L and FLIP2009-18 L) that were resistant under greenhouse and field conditions. © 2011 Springer Science + Business Media B.V. Source


Karimizadeh R.,Dryland Agriculture Research Institute | Mohammadi M.,Dryland Agriculture Research Institute | Shefazadeh M.K.,Islamic Azad University at Yasuj
Journal of Food, Agriculture and Environment | Year: 2011

This study was carried out to determine the yield performances of ten lentil genotypes across five locations in Iran for two years from 2003-2004 to 2004-2005 growing seasons. The goal of this research was to provide biologically meaningful interpretation of genotype-environment (GE) interactions and determine stable genotypes by using adjusted Additive Main effect and Multiplicative Interaction (AMMI) model, environmental variance, b i regression coefficient and Wricke ecovalence. Reliability of genotypes was evaluated with I i index. AMMI ANOVA showed that environments, genotypes and GE interactions were highly significant (P<0.01). F GH1 and F GH2 tests were done for better control of Type-1 error rates. After use of adjusted AMMI, F GH1 and F GH2 indicated only first two IPCA axes of AMMI model were significant at the 0.01 probability level and reminded in the model. The b i regression coefficient showed genotypes 5 (Flip 92-12L) and 10 (Gachsaran) as the genotypes with the greatest stability because of their b value was significantly lower than 1, but genotype 9 (ILL 6199) possessed average stability due to its regression coefficient near to 1(b i = 1.02) and can be consider as well adapted genotype across the environments because good mean yield. Genotypes 8 (Flip 96-9L) and 9 (ILL 6199) with the lowest S 2 and W 2 i values (the most stable genotypes) were also within the highest yielding group, and thus performed as the widely adapted genotypes across the test environments in environmental variance and Wricke ecovalence stability methods. Reliability index of genotypes showed that genotypes 9(ILL 6199) and 1(Flip 96-7L) were the most reliable genotypes and were selected in this research. AMMI Stability Value (ASV) parameter of AMMI model correlated significantly and positively with the S 2 and W 2 i stability parameters (r = 0.851**, r = 0.818**, respectively), also two parameters S 2 and W 2 i showed high and positive correlation (r = 0.997***) and reliability index didn't show any correlation with other parameters. However, genotype 9 with high yield stability in evaluated stability methods, early maturity, high 1000-kernel weight and favorable plant height, was selected for stable and excel genotype in this research. Source

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