Agriculture Biotechnology Research Institute of Iran ABRII

Karaj, Iran

Agriculture Biotechnology Research Institute of Iran ABRII

Karaj, Iran
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Shakeri M.,University Putra Malaysia | Oskoueian E.,Agriculture Biotechnology Research Institute of Iran ABRII | Najafi P.,University Putra Malaysia
Istanbul Universitesi Veteriner Fakultesi Dergisi | Year: 2016

The purpose of this study was to investigate effects of coconut milk supplementation on corticosterone and acute phase protein level under high stocking density. A total 300 Cobb 500 male chicks were placed in cages and stocked as 10 birds/cage (normal stocking density) and 15 birds/cage (high stocking density). The treatments were as (i) control diet and stocked at 10 and 15 birds/cage (ii) control diet + 3% coconut milk from 1-42 day and stocked at 10 and 15 birds/cage (iii) control diet + 5% coconut milk from 1-42 day and stocked at 10 and 15 birds/cage. On day 42, 20 birds per treatment were slaughtered to collect blood samples. The results showed higher level of corticosterone and acute phase protein level in control diet compare to other supplemented diets with coconut milk. In conclusion, coconut milk decreased the level of corticosterone and acute phase protein when chicks were subjected to high stocking density.

Zeinalabedini M.,Agriculture Biotechnology Research Institute of Iran ABRII | Khayam-Nekoui M.,Agriculture Biotechnology Research Institute of Iran ABRII | Grigorian V.,University of Tabriz | Gradziel T.M.,University of California at Davis | Martinez-Gomez P.,CSIC - Center of Edafology and Applied Biology of the Segura
Scientia Horticulturae | Year: 2010

Sixteen nuclear and 10 chloroplast SSR markers were evaluated for 40 almond genotypes including cultivated almond, 18 related species and 5 interspecific-hybrid populations. Results establish the value of SSR (nuclear and chloroplast) markers for distinguishing different genetic lineages and characterize an extensive gene pool available to almond genetic improvement. Hierarchical analysis using integrated nuclear and chloroplast DNA markers support Prunus fenzliana, a species native to the northeast Iran, as a probable ancestor of the cultivated almond. Results also established the importance of interspecific hybridization and subsequent genetic introgression in the development of cultivated almond and demonstrate continuing value of an interspecific gene pool for modern cultivar improvement. Molecular results implicate a dissemination of the cultivated almond from Asia to the Eastern Mediterranean and subsequently the Western Mediterranean and the New World is supported by the molecular analysis of regional germplasm. © 2010 Elsevier B.V.

PubMed | University of Alberta, University Of Ilorin and Agriculture Biotechnology Research Institute of Iran ABRII
Type: | Journal: Journal of agricultural and food chemistry | Year: 2017

Revealing the ruminal fermentation patterns and microbial populations as affected by dietary n-6:n-3 PUFA ratio would be useful for further clarifying the role of the rumen in the lipid metabolism of ruminants. The objective of the present study was to investigate the effects of dietary n-6:n-3 PUFA ratios on fermentation characteristics, fatty acid (FA) profiles, and microbial populations in the rumen of goats. A total of twenty-one goats were randomly assigned to three dietary treatments with different n-6:n-3 PUFA ratios of 2.27:1 (low ratio, LR), 5.01:1 (medium ratio, MR), and 10.38:1 (high ratio, HR). After 100 days of feeding, all goats were slaughtered. Dietary n-6:n-3 PUFA ratios had no effect (P > 0.05) on rumen pH and NH

Valdiani A.,University Putra Malaysia | Javanmard A.,Agriculture Biotechnology Research Institute of Iran ABRII | Talei D.,Shahed University | Tan S.G.,University Putra Malaysia | And 3 more authors.
Molecular Biology Reports | Year: 2013

Andrographis paniculata (AP) is a medicinal plant species introduced into Malaysia. To address the genetic structure and evolutionary connectedness of the Malaysian AP with the Indian AP, a DNA sequence analysis was conducted based on 24 microsatellite markers. Out of the 24 primer sets, seven novel microsatellite primers were designed and amplified intra-specifically according to the available Indian AP sequences at the National Centre for Biotechnology Information (NCBI), where 17 of them were amplified using the cross-species strategy by employing the primers belonging to Acanthus ilicifolius Linn (Acanthaceae) and Lumnitzera racemosa Wild (Combretaceae). The primers were then applied on the Malaysian AP accessions. Sixteen of the new microsatellite loci were amplified successfully. Analysis of these microsatellite sequences, revealed some significant differences between the Indian and Malaysian AP accessions in terms of the size and type of the repeat motifs. These findings depicted the cryptic feature of this species. Despite identifying several heterozygous alleles no polymorphism was observed in the detected loci of the selected accessions. This situation was in concordance with the presence of "fixed heterozygosity" phenomenon in the mentioned loci. Accordingly, this was fully consistent with the occurrence of the genetic bottleneck and founder effect within Malaysian AP population. Apart from the amplification of new microsatellites in this species, our observations could be in agreement with the risk of genetic depletion and consequently extinction of this precious herb in Malaysia. This issue should be taken into consideration in the future studies. © 2012 Springer Science+Business Media Dordrecht.

Khoshkholghsima N.A.,Agriculture Biotechnology Research Institute of Iran ABRII | Rohollahi I.,Shahed University
Horticulture Environment and Biotechnology | Year: 2015

Native grasses are important for the enhancement of landscape. Agropyron cristatum, A.intermedium, Festuca ovina, Festuca arundinaceae, Cynodon dactylon, Bromus inermis and B. confinis are the potential sources of low maintenance turfgrasses for semi-arid regions. This greenhouse study examined the interaction of four different levels of soil water contents with 75, 50, and 25% field capacity (FC) and non-irrigation on physiological and biochemical responses of each accession. Total carbohydrate and sucrose contents of F. arundinaceae were increased significantly under non-irrigated treatment but decreased in the other genotypes. Proline, hydrogen peroxide, and total ascorbate increased in all the species under drought. Also, with decreased soil water content, H2O2 content increased up to 50% in F. arundinaceae. Protein content of F. arundinacea increased significantly under drought stress compared to the control; however, it decreased in other genotypes. Ascorbate peroxidase activities were increased in Festuca sp. and decreased in A. cristatum. It was found that F. arundinacea was able to maintain higher relative water content level and osmotic potential in non-irrigated drought treatment than B. inermis and A. cristatum. It may be speculated that the observed drought stress tolerance was associated with the ability of accumulating compatible solutes and H2O2 signaling cascade. Also, the high activity of ascorbate peroxidase resulted in protection against oxidative damage. It seems that this mechanism worked better in F. arundinacea to be used as low maintenance turfgrass. © 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH.

Tohidfar M.,Agriculture Biotechnology Research Institute of Iran ABRII | Hossaini R.,University of Tabriz | Shokhandan Bashir N.,University of Tabriz | Meisam T.,Agriculture Biotechnology Research Institute of Iran ABRII
Czech Journal of Genetics and Plant Breeding | Year: 2012

The cotton wilt disease caused by the fungus Verticillium dahliae reduces total yield and quality in cotton worldwide. Since traditional breeding has achieved only low levels of wilt resistance, we used a transgenic approach to produce wilt tolerant cotton. We produced fertile transgenic T 2 cotton lines by Agrobacterium-mediated transfer of an endochitinase gene from Phaseolus. Transgene integration and expression were confirmed by PCR amplification, Southern blot and Western blot analyses. Seedlings of homozygous T 2 plants with high levels of chitinase expression were evaluated for their tolerance to Verticillium dahliae by infecting them with a spore suspension in a greenhouse. The transgenic plants demonstrated higher degrees of tolerance to the fungus than non-transgenic plants, as measured by foliar disease symptoms, vascular discoloration and plant height. Transgenic plants were also taller than the control ones and are currently being grown in the greenhouse and will be crossed with Iranian cotton breeding lines in a future study.

Oskoueian E.,University Putra Malaysia | Oskoueian E.,Agriculture Biotechnology Research Institute of Iran ABRII | Abdullah N.,University Putra Malaysia | Ahmad S.,University Putra Malaysia
Molecules | Year: 2012

Jatropha meal produced from the kernel of Jatropha curcas Linn. grown in Malaysia contains phorbol esters (PEs). The potential benefits of PEs present in the meal as anticancer agent are still not well understood. Hence, this study was conducted to evaluate the cytotoxic effects and mode of actions of PEs isolated from Jatropha meal against breast (MCF-7) and cervical (HeLa) cancer cell lines. Isolated PEs inhibited cells proliferation in a dose-dependent manner of both MCF-7 and HeLa cell lines with the IC 50 of 128.6 ± 2.51 and 133.0 ± 1.96 μg PMA equivalents/mL respectively, while the values for the phorbol 12-myristate 13-acetate (PMA) as positive control were 114.7 ± 1.73 and 119.6 ± 3.73 μg/mL, respectively. Microscopic examination showed significant morphological changes that resemble apoptosis in both cell lines when treated with PEs and PMA at IC 50 concentration after 24 h. Flow cytometry analysis and DNA fragmentation results confirmed the apoptosis induction of PEs and PMA in both cell lines. The PEs isolated from Jatropha meal activated the PKC-δ and down-regulated the proto-oncogenes (c-Myc, c-Fos and c-Jun). These changes probably led to the activation of Caspase-3 protein and apoptosis cell death occurred in MCF-7 and HeLa cell lines upon 24 h treatment with PEs and PMA. Phorbol esters of Jatropha meal were found to be promising as an alternative to replace the chemotherapeutic drugs for cancer therapy. © 2012 by the authors.

Hendra R.,University Putra Malaysia | Hendra R.,University of Riau | Ahmad S.,University Putra Malaysia | Oskoueian E.,University Putra Malaysia | And 3 more authors.
BMC Complementary and Alternative Medicine | Year: 2011

Background: Phaleria macrocarpa (Scheff.) Boerl (Thymelaceae) originates from Papua Island, Indonesia and grows in tropical areas. The different parts of the fruit of P. macrocarpa were evaluated for antioxidant, anti-inflammatory, and cytotoxic activities.Methods: Phaleria macrocarpa fruit were divided into pericarp, mesocarp and seed. All parts of the fruit were reflux extracted with methanol. The antioxidant activity of the extracts were characterized in various in vitro model systems such as FTC, TBA, DPPH radical, reducing power and NO radical. Anti-inflammatory assays were done by using NO production by macrophage RAW 264.7 cell lines induced by LPS/IFN-γ and cytotoxic activities were determined by using several cancer cell lines and one normal cell line. Results: The results showed that different parts (pericarp, mesocarp, and seed) of Phaleria macrocarpa fruit contain various amount of total phenolic (59.2 ± 0.04, 60.5 ± 0.17, 47.7 ± 1.04 mg gallic acid equivalent/g DW) and flavonoid compounds (161.3 ± 1.58, 131.7 ± 1.66, 35.9 ± 2.47 mg rutin equivalent/g DW). Pericarp and mesocarp showed high antioxidant activities by using DPPH (71.97%, 62.41%), ferric reducing antioxidant power (92.35%, 78.78%) and NO scavenging activity (65.68%, 53.45%). Ferric thiocyanate and thiobarbituric acid tests showed appreciable antioxidant activity in the percentage hydroperoxides inhibitory activity from pericarp and mesocarp in the last day of the assay. Similarly, the pericarp and mesocarp inhibited inducible nitric oxide synthesis with values of 63.4 ± 1.4% and 69.5 ± 1.4% in macrophage RAW 264.7 cell lines induced by LPS/IFN-γ indicating their notable anti-inflammatory potential. Cytotoxic activities against HT-29, MCF-7, HeLa and Chang cell lines were observed in all parts.Conclusions: These results indicated the possible application of P. macrocarpa fruit as a source of bioactive compounds, potent as an antioxidant, anti inflammatory and cytotoxic agents. © 2011 Hendra et al; licensee BioMed Central Ltd.

Pournourali M.,Guilan University | Tarang A.R.,Agriculture Biotechnology Research Institute of Iran ABRII | Yousefi M.,Guilan University
Cellular and Molecular Biology | Year: 2015

Prostate cancer (PCa) is the most common malignancy in men and the fourth most common cause of death based on cancer all over the world. Many genes has been shown to be involved in the progress of the prostate cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. The aim of this study was to evaluate the association of ApE1 1349T>G polymorphism and the susceptibility to prostate cancer in northern Iran population. Samples were collected from 100 patients diagnosed with prostate cancer patients and 100 controls subjects and genotyped by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). We observed a significant difference in genotype distributions of ApE1 1349T>G polymorphism between patients and controls (P= 0.039). Our findings revealed individuals with the variant TG and GG had a significant increased risk of prostate cancer (GG: OR= 2.50, 95%CI= 1.063-5.874, P= 0.035. TG: OR= 2.40, 95%CI= 1.16-4.95, P= 0.017). Also, more analyses were showed that G allele were associated with increased risk of prostate cancer (OR= 1.493, 95%CI= 1.007-2.21, P= 0.045). The data from this study indicates that the ApE1 1349T>G polymorphism is associated with increased risk of prostate cancer. Although more studies should be considered with larger number of patients and control subjects to confirm our results. © 2015.

PubMed | Agriculture Biotechnology Research Institute of Iran ABRII and Guilan University
Type: Journal Article | Journal: Taiwanese journal of obstetrics & gynecology | Year: 2017

Infertility is a disease of the reproductive system defined by inability to conceive after having regular unprotected intercourse. Both environmental and genetic factors can be involved in female infertility. Manganese superoxide dismutase (MnSOD) is a crucial mitochondrial antioxidant enzyme that has a key role in cellular defense against agents that induce oxidative stress. The present study was aimed to evaluate the MnSOD A16V gene polymorphism in female infertility in northern Iran.Samples were obtained from 150 patients diagnosed with female infertility and 150 controls and genotyped by the polymerase chain reaction-restriction fragment length polymorphism method.The MnSOD genotype frequencies amongst the 150 cases were A/A=27.3%, A/V=69.4%, and V/V=3.3%; the A and V allele frequencies were 62% and 38%, respectively. The MnSOD genotype frequencies amongst the 150 controls were A/A=33.3%, A/V=48.0%, and V/V=18.7%; the A and V allele were 57% and 43%, respectively. We observed a significant difference in genotype distributions of MnSOD A16V polymorphism between patients and controls (p=0.0001).It is suggested that the MnSOD A16V polymorphism may be associated with a risk of female infertility in northern Iran. More studies should be considered with a larger number of patients and controls to confirm our results.

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