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Pournourali M.,Guilan University | Tarang A.R.,Agriculture Biotechnology Research Institute of Iran ABRII | Yousefi M.,Guilan University
Cellular and Molecular Biology | Year: 2015

Prostate cancer (PCa) is the most common malignancy in men and the fourth most common cause of death based on cancer all over the world. Many genes has been shown to be involved in the progress of the prostate cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. The aim of this study was to evaluate the association of ApE1 1349T>G polymorphism and the susceptibility to prostate cancer in northern Iran population. Samples were collected from 100 patients diagnosed with prostate cancer patients and 100 controls subjects and genotyped by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). We observed a significant difference in genotype distributions of ApE1 1349T>G polymorphism between patients and controls (P= 0.039). Our findings revealed individuals with the variant TG and GG had a significant increased risk of prostate cancer (GG: OR= 2.50, 95%CI= 1.063-5.874, P= 0.035. TG: OR= 2.40, 95%CI= 1.16-4.95, P= 0.017). Also, more analyses were showed that G allele were associated with increased risk of prostate cancer (OR= 1.493, 95%CI= 1.007-2.21, P= 0.045). The data from this study indicates that the ApE1 1349T>G polymorphism is associated with increased risk of prostate cancer. Although more studies should be considered with larger number of patients and control subjects to confirm our results. © 2015. Source


Trouillard M.,Institute Of Biologie Physico Chimique | Shahbazi M.,Agriculture Biotechnology Research Institute of Iran ABRII | Moyet L.,French National Center for Scientific Research | Moyet L.,French Atomic Energy Commission | And 12 more authors.
Biochimica et Biophysica Acta - Bioenergetics | Year: 2012

The physiological role of the plastid terminal oxidase (PTOX) involved in plastoquinol oxidation in chloroplasts has been investigated in vivo in tomato leaves. Enzyme activity was assessed by non-invasive methods based on the analysis of the kinetics of chlorophyll fluorescence changes. In the dark, the maximum PTOX rate was smaller than 1 electron per second per PSII. This value was further decreased upon light acclimation, and became almost negligible upon inhibition of the photosynthetic performances by reducing the CO2 availability. In contrast, prolonged exposure to high light resulted in an increase of the overall PTOX activity, which was paralleled by an increased protein accumulation. Under all the conditions tested the enzyme activity always remained about two orders of magnitude lower than that of electron flux through the linear photosynthetic electron pathway. Therefore, PTOX cannot have a role of a safety valve for photogenerated electrons, while it could be involved in acclimation to high light. Moreover, by playing a major role in the control of the stromal redox poise, PTOX is also capable of modulating the balance between linear and cyclic electron flow around PSI during the deactivation phase of carbon assimilation that follows a light to dark transition. © 2012 Elsevier B.V. Source


Khoshkholghsima N.A.,Agriculture Biotechnology Research Institute of Iran ABRII | Rohollahi I.,Shahed University
Horticulture Environment and Biotechnology | Year: 2015

Native grasses are important for the enhancement of landscape. Agropyron cristatum, A.intermedium, Festuca ovina, Festuca arundinaceae, Cynodon dactylon, Bromus inermis and B. confinis are the potential sources of low maintenance turfgrasses for semi-arid regions. This greenhouse study examined the interaction of four different levels of soil water contents with 75, 50, and 25% field capacity (FC) and non-irrigation on physiological and biochemical responses of each accession. Total carbohydrate and sucrose contents of F. arundinaceae were increased significantly under non-irrigated treatment but decreased in the other genotypes. Proline, hydrogen peroxide, and total ascorbate increased in all the species under drought. Also, with decreased soil water content, H2O2 content increased up to 50% in F. arundinaceae. Protein content of F. arundinacea increased significantly under drought stress compared to the control; however, it decreased in other genotypes. Ascorbate peroxidase activities were increased in Festuca sp. and decreased in A. cristatum. It was found that F. arundinacea was able to maintain higher relative water content level and osmotic potential in non-irrigated drought treatment than B. inermis and A. cristatum. It may be speculated that the observed drought stress tolerance was associated with the ability of accumulating compatible solutes and H2O2 signaling cascade. Also, the high activity of ascorbate peroxidase resulted in protection against oxidative damage. It seems that this mechanism worked better in F. arundinacea to be used as low maintenance turfgrass. © 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH. Source


Oskoueian E.,University Putra Malaysia | Oskoueian E.,Agriculture Biotechnology Research Institute of Iran ABRII | Abdullah N.,University Putra Malaysia | Ahmad S.,University Putra Malaysia
Molecules | Year: 2012

Jatropha meal produced from the kernel of Jatropha curcas Linn. grown in Malaysia contains phorbol esters (PEs). The potential benefits of PEs present in the meal as anticancer agent are still not well understood. Hence, this study was conducted to evaluate the cytotoxic effects and mode of actions of PEs isolated from Jatropha meal against breast (MCF-7) and cervical (HeLa) cancer cell lines. Isolated PEs inhibited cells proliferation in a dose-dependent manner of both MCF-7 and HeLa cell lines with the IC 50 of 128.6 ± 2.51 and 133.0 ± 1.96 μg PMA equivalents/mL respectively, while the values for the phorbol 12-myristate 13-acetate (PMA) as positive control were 114.7 ± 1.73 and 119.6 ± 3.73 μg/mL, respectively. Microscopic examination showed significant morphological changes that resemble apoptosis in both cell lines when treated with PEs and PMA at IC 50 concentration after 24 h. Flow cytometry analysis and DNA fragmentation results confirmed the apoptosis induction of PEs and PMA in both cell lines. The PEs isolated from Jatropha meal activated the PKC-δ and down-regulated the proto-oncogenes (c-Myc, c-Fos and c-Jun). These changes probably led to the activation of Caspase-3 protein and apoptosis cell death occurred in MCF-7 and HeLa cell lines upon 24 h treatment with PEs and PMA. Phorbol esters of Jatropha meal were found to be promising as an alternative to replace the chemotherapeutic drugs for cancer therapy. © 2012 by the authors. Source


Hendra R.,University Putra Malaysia | Hendra R.,University of Riau | Ahmad S.,University Putra Malaysia | Oskoueian E.,University Putra Malaysia | And 3 more authors.
BMC Complementary and Alternative Medicine | Year: 2011

Background: Phaleria macrocarpa (Scheff.) Boerl (Thymelaceae) originates from Papua Island, Indonesia and grows in tropical areas. The different parts of the fruit of P. macrocarpa were evaluated for antioxidant, anti-inflammatory, and cytotoxic activities.Methods: Phaleria macrocarpa fruit were divided into pericarp, mesocarp and seed. All parts of the fruit were reflux extracted with methanol. The antioxidant activity of the extracts were characterized in various in vitro model systems such as FTC, TBA, DPPH radical, reducing power and NO radical. Anti-inflammatory assays were done by using NO production by macrophage RAW 264.7 cell lines induced by LPS/IFN-γ and cytotoxic activities were determined by using several cancer cell lines and one normal cell line. Results: The results showed that different parts (pericarp, mesocarp, and seed) of Phaleria macrocarpa fruit contain various amount of total phenolic (59.2 ± 0.04, 60.5 ± 0.17, 47.7 ± 1.04 mg gallic acid equivalent/g DW) and flavonoid compounds (161.3 ± 1.58, 131.7 ± 1.66, 35.9 ± 2.47 mg rutin equivalent/g DW). Pericarp and mesocarp showed high antioxidant activities by using DPPH (71.97%, 62.41%), ferric reducing antioxidant power (92.35%, 78.78%) and NO scavenging activity (65.68%, 53.45%). Ferric thiocyanate and thiobarbituric acid tests showed appreciable antioxidant activity in the percentage hydroperoxides inhibitory activity from pericarp and mesocarp in the last day of the assay. Similarly, the pericarp and mesocarp inhibited inducible nitric oxide synthesis with values of 63.4 ± 1.4% and 69.5 ± 1.4% in macrophage RAW 264.7 cell lines induced by LPS/IFN-γ indicating their notable anti-inflammatory potential. Cytotoxic activities against HT-29, MCF-7, HeLa and Chang cell lines were observed in all parts.Conclusions: These results indicated the possible application of P. macrocarpa fruit as a source of bioactive compounds, potent as an antioxidant, anti inflammatory and cytotoxic agents. © 2011 Hendra et al; licensee BioMed Central Ltd. Source

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