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Sevik M.,Veterinary Control Institute | Avc O.,Selcuk University | Ince O.B.,Agriculture and Rural Development Support Institution
Tropical Animal Health and Production | Year: 2015

Enzootic bovine leukosis (EBL) which is caused by bovine leukaemia virus (BLV) has an important economic impact on dairy herds due to reduced milk production and restrictions on livestock exports. This study was conducted to determine the BLV infection status in Central Anatolia Region of Turkey, an important milk production centre, and to examine the risk factors such as purchasing cattle, increasing cattle age, cattle breed and herd size associated with transmission of BLV infection. To estimate the rate of BLV infection, a survey for specific antibodies in 28,982 serum samples from animals belonging to 1116 different herds situated in Central Anatolia Region of Turkey were tested from January 2006 to December 2013. A generalized mixed linear model was used to evaluate the risk factors that influenced BLV seroprevalence. Antibodies against BLV were detected in 431 (2.28 %) of 18,822 Holstein and 29 (0.28 %) of 10,160 Brown Swiss cows. Among 1116 herds, 132 herds (11.82 %) had one or more positive animals. Also results of our study show that the prevalence of BLV infection increased from 2006 to 2011, and it tends to reduce with BLV control programme. Furthermore, we found positive associations between percentage of seropositive animal and increasing cattle age, herd size, cattle breed and purchased cattle. Age-specific prevalence showed that BLV prevalence increased with age. These factors should be taken into consideration for control of BLV infection. © 2015, Springer Science+Business Media Dordrecht. Source

Sevik M.,Veterinary Control Institute | Avci O.,Selcuk University | Dogan M.,Veterinary Control Institute | Ince O.B.,Agriculture and Rural Development Support Institution
BioMed Research International | Year: 2016

Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n = 15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n = 15). A quantitative real-time PCR method was used to detect Capripoxvirus (CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection. © 2016 Murat Şevik et al. Source

Koca S.B.,Suleyman Demirel University of Turkey | Yigit N.O.,Suleyman Demirel University of Turkey | Uzunmehmetoglu E.,Suleyman Demirel University of Turkey | Guclu Z.,Agriculture and Rural Development Support Institution | And 2 more authors.
Israeli Journal of Aquaculture - Bamidgeh | Year: 2015

This experiment was conducted to investigate the effects Daphnia magna enriched with lipid emulsions as an alternative to Artemia, on growth, survival rate, and fatty acid composition of freshwater crayfish (Astacus leptodactylus Esch. 1823). The four treatment groups were (a) unenriched Artemia (UEA), (b) unenriched D. magna (UED), (c) D. magna enriched with redpepper emulsion (DER), and (d) D. magna enriched with anchovy oil emulsion (DEA). All tests were carried out in triplicate for 30 days. The crayfish (mean weight 0.12g) were fed ad libitum once daily. At the end of experiment, the highest eicosapentaenoic acid, 20:5n-3 (EPA) level was found in the DEA group (5.77%). The highest DHA (docosahexaenoic acid, 22:6n-3) level was found in the DER group (2.73%) which was statistically similar to the DEA group. In addition, high n-3 HUFA (high unsaturated fatty acid) levels were detected in enriched D. magna groups with emulsions. However, high EPA levels in enriched D. magna groups with emulsions were not reflected in crayfish tissues, but DHA level was reflected in crayfish tissues fed with anchovy oil emulsion. The crayfish fed with D. magna showed similar growth to that of the Artemia fed groups. The growth of the enriched D. magna groups did not differ. © 2015, Israeli Journal of Aquaculture - Bamidgeh. All rights reserved. Source

Guclu S.S.,Suleyman Demirel University of Turkey | Kucuk F.,Suleyman Demirel University of Turkey | Ertan O.O.,Suleyman Demirel University of Turkey | Guclu Z.,Agriculture and Rural Development Support Institution
Turkish Journal of Fisheries and Aquatic Sciences | Year: 2013

In order to establish the taxonomic and zoogeographic features of the fish fauna of the Büyük Menderes River basin, fish samples were collected using electrofishing equipment, gill nets, trammel nets, seine nets and cast nets from 2010 to 2011. Among the samples, 34 (19 endemic and 6 non-native species) species belonging to 13 families were identified. The most dominant family in the river and its branches is the Cyprinidae with 20 species. Rhodeus amarus, Büyük Menderes River has been reported for the first time. © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey. Source

Dundar A.,Agriculture and Rural Development Support Institution | Saricoban C.,Selcuk University | Yilmaz M.T.,Yildiz Technical University
Meat Science | Year: 2012

A five-factor Central Composite Orthogonal Design was adopted to study simultaneous effects of some processing variables such as NaCl (0-2%), fat (10-30%), ascorbic acid (0-600. ppm), cooking temperature (150-230. °C) and cooking time (5-15. min) on physicochemical properties and heterocyclic aromatic amine (HAA) contents of cooked beef patties. The HAAs analyzed were 2-amino-3-methylimidazo[4,5-f]-quinoline (IQ), 2-amino-3,8-dimethylimidazo[4,5-f]-quinoxaline (MeIQx), 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline (MeIQ), 2-amino-3,4,8-trimethylimidazo[4,5-f]-quinoxaline (4,8-DiMeIQx) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]-pyridine (PhIP), as quantified by high-performance liquid chromatography with photo-diode array detection (HPLC-UV/DAD). It was found that ascorbic acid decreased; however, fat, cooking temperature and time levels increased the contents of IQ, MeIQx, MeIQ and PhIP. In addition, estimated ridge analysis was conducted to find values of the processing variables that maximize and minimize the five HAA contents, revealing that the results obtained would be useful for meat industry aiming to decrease HAA content in cooked meat products. © 2012 Elsevier Ltd. Source

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