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Zahirian A.,Islamic Azad University | Padash A.,Islamic Azad University | Jozi S.A.,Islamic Azad University at Tehran | Zamanpour M.,Fars Agriculture and Natural Resources Research Center | Nabavi S.M.B.,Iran University of Science and Technology
Advances in Environmental Biology | Year: 2012

Wetlands with high biomass production and water storage, in addition to supply a part of the food and water required for local people, impose positive effects on their economy, customs and culture. On one hand, migration of different species of birds to the wetlands and on the other hand, high diversity of flora in different plant categories emphasize on the need of proper planning for conservation of biodiversity in the wetlands. Wetland zonation to protect the biological resources is one of the basic steps in this respect. Parishan Wetland with an area of 4800 ha, is one of the permanent freshwater wetlands in Iran, located 12 Km away from the southeast of Kazeroun City in Fars Province. The basic purpose of zoning Parishan Wetland is to conserve biodiversity and develop ecotourism in the region with regard to environmental and ecosystem aspects. Multi Criteria Evaluation (MCE) has been used for this purpose. After identifying the available resources, two protected and recreational zones were identified. The recreational zone, in turn, was divided into a central zone and two proposed zone (1 and 2). All three recreational zones were located on the northwest of Parishan Lake and the rest of the lake area was assigned to conservation zone. The obtained results indicated that, conservation zone is broader than recreational zones. Source

Allahyari M.,Fars Agriculture and Natural Resources Research Center | Bandani A.R.,University of Tehran | Habibi-Rezaei M.,University of Tehran
Journal of Insect Physiology | Year: 2010

The subcellular distributions of six digestive and non-digestive enzymes (α-glucosidase, β-glucosidase, alkaline phosphatase, acid phosphatase, aminopeptidase and lactate dehydrogenase) of Eurygaster integriceps have been studied. The subcellular distributions of acid phosphatase and α-glucosidase are similar and the gradient ultracentrifugation profiles of these two enzymes overlap. Two partially membrane-bound enzymes, alkaline phosphatase and β-glucosidase have similar distributions in differential centrifugation fractions, which are different from that of α-glucosidase. Sucrose gradient ultracentrifugation of membranes from luminal contents showed that β-glucosidase carrying membranes are heavier. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the profile of proteins extracted from β-glucosidase carrying membranes is different from that of α-glucosidase carrying membranes. We conclude that β-glucosidase and aminopeptidase are markers of microvillar membrane (MM) and perimicrovillar space, respectively, while α-glucosidase and acid phosphatase are perimicrovillar markers. In E. integriceps V1 luminal content is a rich source of PMM and MM and that is used to resolve these membranes. © 2009 Elsevier Ltd. Source

Faghihi M.M.,Hormozgan Agriculture and Natural Resources Research Center | Bagheri A.N.,Hormozgan Agriculture and Natural Resources Research Center | Bahrami H.R.,Hormozgan Agriculture and Natural Resources Research Center | Hasanzadeh H.,Hormozgan Agriculture and Natural Resources Research Center | And 5 more authors.
Plant Disease | Year: 2011

Witches'-broom disease of lime (WBDL), caused by 'Candidatus Phytoplasma aurantifolia', has devastated many Mexican lime orchards and is currently a threat to lime production in neighboring provinces of southern Iran. Several reports have suggested transmission and spread of WBDL phytoplasma through the seed of infected plants. In the present study, claims of seed transmission of this phytoplasma were examined. Fruit were collected from infected trees in the infested areas of Minab (Hormozgan Province) and from symptomless trees in noninfested areas. Lime seed from symptomless and witches'-broom-affected trees were sown in separate beds in an insect-proof screenhouse and the resulting seedlings were examined for phytoplasmal infection. Leaf, stem, and root samples were collected from both groups of seedlings every 3 months for 2 years and tested for WBDL phytoplasma using direct and nested polymerase chain reaction (PCR). Repeated PCR tests on the seedlings did not reveal the presence of phytoplasmal DNA. Likewise, symptoms of the disease were not observed on these seedlings after 2 years. PCR assays detected the phytoplasma in coats of some seed from infected trees; however, no excised embryos were positive for the phytoplasma. All positive PCR results were confirmed by restriction fragment length polymorphism assay. One-year-old seedlings derived from seed of noninfected plants appeared more vigorous in terms of height, number of leaves, and fresh weight of shoot compared with those from infected trees. The germination percentage, mean daily germination, peak value, and germination value were significantly higher for seed of fruit from noninfected trees and seed from fruit on asymptomatic branches of infected trees than those from fruit on symptomatic branches of infected trees. © 2011 The American Phytopathological Society. Source

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