Baoding, China

Agricultural University of Hebei is a university in Baoding, Hebei, China under the provincial government. Wikipedia.

Time filter

Source Type

Li X.,Yanshan University | Zhong F.,Agricultural University of Hebei
Inflammation | Year: 2014

Exposure to nickel (Ni2+) can trigger allergic reactions in susceptible individuals, which is widely accepted as the major cause of allergic contact hypersensitivity (CHS) worldwide. Although Ni2+-induced proinflammatory responses clearly play a pivotal role in CHS, the underlying molecular mechanism has not been fully defined. Here we report that Ni 2+ activates the NLRP3-ASC-caspase-1 immune signaling pathway in antigen-presenting cells, leading to the proteolytic processing and secretion of a proinflammatory cytokine, interleukin-1β (IL-1β). The activation of this signaling axis is independent of phagolysosome-cathepsin B pathway. Instead, Ni2+ induces mitochondrial reactive oxygen species accumulation and cation fluxes, both of which are required for activating the NLRP3-ASC-caspase-1 pathway. Together, these results identified a novel innate immune signaling pathway (NLRP3-ASC-caspase-1-IL-1β) activated by Ni 2+ and provided a mechanistic basis for optimizing the therapeutic intervention against Ni2+-induced allergy in patients. © 2013 Springer Science+Business Media New York.

Wu Q.,Agricultural University of Hebei | Zhao G.,Agricultural University of Hebei | Feng C.,Agricultural University of Hebei | Wang C.,Agricultural University of Hebei | Wang Z.,Agricultural University of Hebei
Journal of Chromatography A | Year: 2011

A graphene-based magnetic nanocomposite was synthesized and used for the first time as an effective adsorbent for the preconcentration of the five carbamate pesticides (metolcarb, carbofuran, pirimicarb, isoprocarb and diethofencarb) in environmental water samples prior to high performance liquid chromatography-diode array detection. The properties of the magnetic nanocomposite were characterized by scanning electron microscopy and X-ray diffraction. This novel graphene-based magnetic nanocomposite showed great adsorptive ability towards the analytes. The method, which takes the advantages of both nanoparticle adsorption and magnetic phase separation from the sample solution, could avoid some of the time-consuming experimental procedures related to the traditional solid phase extraction. Various experimental parameters that could affect the extraction efficiencies have been investigated. Under the optimum conditions, the enrichment factors of the method for the analytes were in the range from 474 to 868. A linear response was achieved in the concentration range of 0.1-50ngmL -1. The limits of detection of the method at a signal to noise ratio of 3 for the pesticides were 0.02-0.04ngmL -1. Compared with the dispersive liquid-liquid microextraction and the ultrasound-assisted surfactant-enhanced emulsification microextraction, much higher enrichment factors and sensitivities were achieved with the developed method. The method has been successfully applied for the determination of the carbamate pesticides in environmental water samples. © 2011 Elsevier B.V.

Our study is to analyse the coinfection effects of porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) in vivo on phagocytosis and interferon mRNA expression level of porcine alveolar macrophages (PAM). Forty-eight 5-week-old healthy piglets were divided randomly into 4 groups of 12 (PCV2, PPV, PCV2/PPV and the control groups). The piglets in PCV2 group were inoculated oronasally with 3 mL of porcine circovirus type2 (PCV2, 10(5.61) TCID50/0.1 mL), PPV group with 3 mL of porcine parvovirus (PPV, 10(6.69) TCID50/0.1 mL), PCV2/PPV group with 3 mL of PCV2 and 3 mL of PPV and the control group with 3 mL of cell culture medium, respectively. Three piglets from each group were sacrificed randomly on 3, 7, 14 and 35 day post infection (dpi) and porcine alveolar macrophages (PAM) were collected to detect viability, phagocytotic capabilities and alpha1- and gamma-interferon (IFN-alpha1 and IFN-gamma) mRNA levels of PAM. The viabilities of PAM from PCV2 group and PCV2/PPV group became weaker than that of control group during the period of 3 - 14 dpi but they were similar to that of control group on 35 dpi; there was no significant difference between the viability of PCV2/PPV group and that of PCV2 group (P > 0.05). The phagocytotic capabilities of PAM from three virus infection groups were lower than that of control group (P < 0.01), among which that of PCV2/PPV group descended more drastically. IFN-alpha1 and IFN-gamma mRNA levels in PAM from PCV2/PPV group were significantly lower than those of PCV2, PPV and control groups (P < 0.01). PCV2/PPV co-infection did not cause further decline of PAM viability but strongly weakened phagocytosis and constantly lowered IFN (IFN-alpha1 and IFN-gamma) mRNA expression levels of PAM.

Du L.,Agricultural University of Hebei
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

To construct and characterize a sigK gene disruption mutant of Bacillus thuringiensis and to study influence of sigK gene disruption on the activation of cry3A gene promoter. We constructed the sigK gene disruption mutant HD delta sigK by inserting kanamycin resistance gene via homologous recombination. Scanning electron microscopy and spore formation analysis were used to detect the abilities of sporulation and crystal protein formation of both the mutant and the wild-type strain. SDS-PAGE analysis was used to detect the expression of crystal protein. Beta-galactosidase assay of cry3A'-lacZ gene fusion was performed to analyze the influence of sigK gene disruption on the activation of cry3A promoter. The growth curve showed that mutant grew slowly in late stationary phase compared to the wild-type strain. Scanning electron microscopy and spore formation analysis indicated that no spore was produced in sigK disruption mutant. SDS-PAGE results exhibited that the expression of cry gene was significantly decreased in the mutant. Beta-galactosidase assay showed that the activation of cry3A promoter was stronger in the mutant than that in HD-73 during late stationary phase, but the disruption of sigK gene had no significant influence on the production of Cry1Ac which was initiated by cry3A gene promoter. These results indicated that sigK gene was one of the essential genes during the sporulation of Bacillus thuringiensis, and influenced the expression of crystal protein. The expression of crystal protein which was initiated by cry3A gene promoter in sigK disruption mutant could be used to develop high-efficiency and safe biological pesticides.

Wang Y.,Agricultural University of Hebei | Gao S.,Agricultural University of Hebei | Zang X.,Agricultural University of Hebei | Li J.,Agricultural University of Hebei | Ma J.,Agricultural University of Hebei
Analytica Chimica Acta | Year: 2012

Graphene, a novel class of carbon nanostructures, has great promise for use as sorbent materials because of its ultrahigh specific surface area. A new method using a column packed with graphene as sorbent was developed for the preconcentration of trace amounts of lead (Pb) using dithizone as chelating reagent prior to its determination by flame atomic absorption spectrometry. Some effective parameters on the extraction and complex formation were selected and optimized. Under optimum conditions, the calibration graph was linear in the concentration range of 10.0-600.0μgL -1 with a detection limit of 0.61μgL -1. The relative standard deviation for ten replicate measurements of 20.0 and 400.0μgL -1 of Pb were 3.56 and 3.25%, respectively. Comparative studies showed that graphene is superior to other adsorbents including C18 silica, graphitic carbon, and single- and multi-walled carbon nanotubes for the extraction of Pb. The proposed method was successfully applied in the analysis of environmental water and vegetable samples. Good spiked recoveries over the range of 95.3-100.4% were obtained. This work not only proposes a useful method for sample preconcentration, but also reveals the great potential of graphene as an excellent sorbent material in analytical processes. © 2011 Elsevier B.V.

Huang Y.,CAS Research Center for Eco Environmental Sciences | Chen Z.,CAS Research Center for Eco Environmental Sciences | Liu W.,Agricultural University of Hebei
Plant and Soil | Year: 2012

Background and Aims: Characteristically baseline levels of Sb in the environment are low, but problematic local elevation trends arise from anthropogenic activities such as mining and incineration. Arsenic (analog of Sb) accumulation by rice can be reduced by iron (Fe) plaque. A hydroponic experiment was conducted to investigate whether Fe plaque could reduce the uptake and translocation of different Sb species in different rice cultivars. Methods: After Fe plaque on rice roots was induced in solution containing 0, 0.2, 0.4, 0.7, 1.2, 2.0 mM Fe 2+ for 24 h, seedlings were transferred into nutrient solution with 20 μM Sb(V) or Sb(III) for 3 d. Results: About 60-80% (Sb(III) treatment) and 40-60% (Sb(V) treatment) of the total Sb accumulated in Fe plaque. There was a significant correlation between the concentrations of Sb and Fe on the root surface. A similar relationship was observed in roots and shoots. Cultivar (Jiahua 1) formed the most Fe plaque, had the highest Fe associated Sb sequestration but the lowest Sb concentration in the root interior. Conclusions: Fe plaque may act as a 'buffer' for Sb(V) and Sb(III) in the rhizosphere, and cultivars played an important role in the different species Sb uptake and translocation. © 2011 Springer Science+Business Media B.V.

Pan H.,Agricultural University of Hebei
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012

To investigate the effects of canine parvovirus (CPV) non-structural protein-1 (NS1) on the cell apoptosis induced by CPV and preliminarily explore the mechanism of CPV-induced apoptosis. First, the NS1 gene was amplified by PCR from CPV genomic DNA and subcloned into pcDNA3. 1A vector to generate NS1 eukaryotic expression vector pcDNA-NS1. To verify whether pcDNA-NS1 vector can mediate NS1 expression in eukaryotic cells, the human embryo kideny (HEK) 293FT cells were used to transiently express the recombinant NS1. The effects of NS1 on CPV-induced apoptosis were investigated by infecting the F81 host cells with CPV and transfecting the cells with NS1 vector. The apoptosis of the cells was detected by AnnexinV/PI double staining for phosphatidylserine externalization on membrane and by luminescence method for caspase-3/7 activities. The results show that the sequence of NS1 gene amplified was consistent with the GenBank. The NS1 expression vector was shown to be correct and could mediate NS1 expression in eukaryotic cells. The phosphatidylserine on outside of membrane was detected and the caspase-3/7 activities were increased in both CPV-infected cells and NS1-transfected cells. These results indicate that both CPV and NS1 protein can induce the apoptosis of the cells. CPV-induced apoptosis was closely related to its non-structural protein NS1.

Shi H.-Y.,Agricultural University of Hebei | Zhang Y.-X.,Agricultural University of Hebei
Molecular Biology Reports | Year: 2012

1-Aminocyclopropane-1-carboxylate (ACC) oxidase catalyzes the final reaction of the ethylene biosynthetic pathway, converting ACC into ethylene. Past studies have shown a possible link between ACC oxidase and salicylic acid during fruit ripening in pear, but the relationship has received no more than modest study at the gene expression level. In this study, two cDNA clones encoding putative ACC oxidase, PpACO1 and PpACO2, were isolated from a cDNA library constructed by our own laboratory and produced using mRNA from mesocarp of pear (Pyrus pyrifolia Nakai. cv.Whangkeumbae). One cDNA clone, designated PpACO1 (GenBank accession No. JN807390), comprised an open reading frame of 945 bp encoding a protein of 314 amino acids. The other cDNA, designated PpACO2 (GenBank accession No. JN807392), encodes a protein with 322 amino acids that shares high similarity with the known plant ACOs. Using PCR amplification techniques, two genomic clones corresponding to PpACO1 and PpACO2 were isolated and shown to contain independently three introns with typical GT/AG boundaries defining the splice junctions. The PpACO1 gene product shared 99 % identity with an ACC oxidase from pear (Pyrus 9 bretschneideri, and phylogenetic analyses clearly placed the gene product in the ACC oxidase cluster of the pear 2-oxoglutarate-dependent dioxygenase superfamily tree. Quantitative RT-PCR analysis indicated that the two PpACO genes are differentially expressed in pear tissues. PpACO1 and PpACO2 were predominantly expressed in fruit. The transcripts of PpACO1 were accumulated at relatively low levels in early fruit, but strongly high levels in fruit ripening and senescence stages, while the transcripts of PpACO2 were accumulated at higher levels in early fruit and much lower levels with further fruit cell development than the transcripts of PpACO1. In addition, PpACO1 gene was down-regulated in fruit by salicylic acid (SA). Nevertheless, PpACO2 gene was dramatically up-regulated in fruit by SA. These results suggested that the PpACOs may participate in regulation of fruit ripening and in response to SA in pear. © Springer Science+Business Media B.V. 2012.

Zhang K.,Agricultural University of Hebei
Applied Mechanics and Materials | Year: 2014

This paper introduces the working principle of remote access system based on SSL VPN in cloud computing. Meanwhile, it focuses on the operation principle of the SSL protocol and resource management. In addition, it analyses system characteristics such as https login, hardware signature certification and so on. At last, it puts forward some problems and solutions in actual use. © (2014) Trans Tech Publications, Switzerland.

Wu Q.,Agricultural University of Hebei | Feng C.,Agricultural University of Hebei | Wang C.,Agricultural University of Hebei | Wang Z.,Agricultural University of Hebei
Colloids and Surfaces B: Biointerfaces | Year: 2013

A superparamagnetic graphene-Fe3O4 nanocomposite (G/Fe3O4) was synthesized by a facile one-pot solvothermal method. The nanocomposite G/Fe3O4 prepared by the new method was firstly used as an adsorbent to remove dye for water pollution remediation. In comparison with G/Fe3O4 prepared by the in situ chemical coprecipitation, the newly prepared G/Fe3O4 had a higher adsorption efficiency for the dye. The adsorption characteristics of the nanocomposite adsorbent were examined using the organic dye pararosaniline as the adsorbate. The adsorption kinetics, adsorption capacity of the adsorbent, and the effect of the adsorbent dosage and solution pH on the removal efficiency of pararosaniline were investigated. The adsorption capacity of G/Fe3O4 for pararosaniline was evaluated using the Freundlich and Langmuir adsorption isotherm models. The G/Fe3O4 hybrid composite can be easily manipulated in magnetic field for desired separation, leading to an easy removal of the dye from polluted water. The G/Fe3O4 hybrid composite would have a great potential in removing organic dyes from polluted water. © 2012 Elsevier B.V.

Loading Agricultural University of Hebei collaborators
Loading Agricultural University of Hebei collaborators