Dong Y.,Agricultural University of Hebei
RISTI - Revista Iberica de Sistemas e Tecnologias de Informacao | Year: 2016
In order to improve the professional competence of college students, we established a multi-dimensional occupational ability model and a vocational competence evaluation system that was based on sequence clustering and hidden Markov chain theory. Through data mining technology, this paper anticipated the development tendency of vocational capability, which is aimed at providing students with more professional training guidance in terms of vocational ability.
Zhao Y.-P.,Agricultural University of Hebei
Proceedings - 2016 International Conference on Robots and Intelligent System, ICRIS 2016 | Year: 2016
Education,service, management and transaction are the responsibilities of counselors. It covers the breadth of the knowledge of any professional teacher,and it also determines the knowledge,theory and method of the subject, which is necessary to support the knowledge,theory and method. The subjectivity of the counselors is necessarily a subject system supported by a multidisciplinary integrated system. With the advantage of multidisciplinary supported the diversity of counselor work,it can help instructors to adapt to the diversified work,and improve the professional level of the work of counselors. © 2016 IEEE.
Li J.,Agricultural University of Hebei
RISTI - Revista Iberica de Sistemas e Tecnologias de Informacao | Year: 2016
This paper mainly adopts the research method of combining theory with practice as well as integration of analysis and synthesis, discusses the related problems of college students' ideological and political education under the context of network communication, and analyzes the prominent features of network communication compared with traditional communication methods, points out that the life picture constituted by network communication already became an important discourse context for college students' ideological and political education, further from the three aspects of students self-management level is not high, ideological and political education has paid less attention to the development of network communication, network environment supervision system is not perfect, this paper explores the causes of the low effectiveness of college students' ideological and political education and the declining of prestige, and finds out the root cause of the "network aphasia" in ideological and political education. On this basis, the effective path of enhancing ideological and political education under the context of network communication is proposed. © AISTI 2016.
Agricultural University of Hebei and Hebei Zhongnongboyuan Agricultural Equipment Co. | Date: 2015-12-10
A facility agricultural laser measure and control motor rotary tillage flattening machine includes a front axle part, a lifting equipment, a rotary tillage component, a battery pack, a rear axle steering part, a rotary flattening component and a rack. A front axle housing with the rack. The rotary tillage component comprises a DC motor, a gearbox and a rotary tillage blade. The DC motor connects with the gearbox through transmission. An output shaft of the gearbox connects with the rotary tillage blade. The rear axle steering part includes a rotary seat, a rotary shaft, a steering handlebar, a rear axle stand and squirrel-cage hubs. An upper end of the rotary shaft is equipped with a steering handlebar. The rear axle stand is equipped with two squirrel-cage hubs through a supporting axle. The rotary flattening component connects with the rack through an electromotive handspike and a flexible torsion bar.
Li X.,Yanshan University |
Zhong F.,Agricultural University of Hebei
Inflammation | Year: 2014
Exposure to nickel (Ni2+) can trigger allergic reactions in susceptible individuals, which is widely accepted as the major cause of allergic contact hypersensitivity (CHS) worldwide. Although Ni2+-induced proinflammatory responses clearly play a pivotal role in CHS, the underlying molecular mechanism has not been fully defined. Here we report that Ni 2+ activates the NLRP3-ASC-caspase-1 immune signaling pathway in antigen-presenting cells, leading to the proteolytic processing and secretion of a proinflammatory cytokine, interleukin-1β (IL-1β). The activation of this signaling axis is independent of phagolysosome-cathepsin B pathway. Instead, Ni2+ induces mitochondrial reactive oxygen species accumulation and cation fluxes, both of which are required for activating the NLRP3-ASC-caspase-1 pathway. Together, these results identified a novel innate immune signaling pathway (NLRP3-ASC-caspase-1-IL-1β) activated by Ni 2+ and provided a mechanistic basis for optimizing the therapeutic intervention against Ni2+-induced allergy in patients. © 2013 Springer Science+Business Media New York.
Wu Q.,Agricultural University of Hebei |
Zhao G.,Agricultural University of Hebei |
Feng C.,Agricultural University of Hebei |
Wang C.,Agricultural University of Hebei |
Wang Z.,Agricultural University of Hebei
Journal of Chromatography A | Year: 2011
A graphene-based magnetic nanocomposite was synthesized and used for the first time as an effective adsorbent for the preconcentration of the five carbamate pesticides (metolcarb, carbofuran, pirimicarb, isoprocarb and diethofencarb) in environmental water samples prior to high performance liquid chromatography-diode array detection. The properties of the magnetic nanocomposite were characterized by scanning electron microscopy and X-ray diffraction. This novel graphene-based magnetic nanocomposite showed great adsorptive ability towards the analytes. The method, which takes the advantages of both nanoparticle adsorption and magnetic phase separation from the sample solution, could avoid some of the time-consuming experimental procedures related to the traditional solid phase extraction. Various experimental parameters that could affect the extraction efficiencies have been investigated. Under the optimum conditions, the enrichment factors of the method for the analytes were in the range from 474 to 868. A linear response was achieved in the concentration range of 0.1-50ngmL -1. The limits of detection of the method at a signal to noise ratio of 3 for the pesticides were 0.02-0.04ngmL -1. Compared with the dispersive liquid-liquid microextraction and the ultrasound-assisted surfactant-enhanced emulsification microextraction, much higher enrichment factors and sensitivities were achieved with the developed method. The method has been successfully applied for the determination of the carbamate pesticides in environmental water samples. © 2011 Elsevier B.V.
Du L.,Agricultural University of Hebei
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011
To construct and characterize a sigK gene disruption mutant of Bacillus thuringiensis and to study influence of sigK gene disruption on the activation of cry3A gene promoter. We constructed the sigK gene disruption mutant HD delta sigK by inserting kanamycin resistance gene via homologous recombination. Scanning electron microscopy and spore formation analysis were used to detect the abilities of sporulation and crystal protein formation of both the mutant and the wild-type strain. SDS-PAGE analysis was used to detect the expression of crystal protein. Beta-galactosidase assay of cry3A'-lacZ gene fusion was performed to analyze the influence of sigK gene disruption on the activation of cry3A promoter. The growth curve showed that mutant grew slowly in late stationary phase compared to the wild-type strain. Scanning electron microscopy and spore formation analysis indicated that no spore was produced in sigK disruption mutant. SDS-PAGE results exhibited that the expression of cry gene was significantly decreased in the mutant. Beta-galactosidase assay showed that the activation of cry3A promoter was stronger in the mutant than that in HD-73 during late stationary phase, but the disruption of sigK gene had no significant influence on the production of Cry1Ac which was initiated by cry3A gene promoter. These results indicated that sigK gene was one of the essential genes during the sporulation of Bacillus thuringiensis, and influenced the expression of crystal protein. The expression of crystal protein which was initiated by cry3A gene promoter in sigK disruption mutant could be used to develop high-efficiency and safe biological pesticides.
Pan H.,Agricultural University of Hebei
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012
To investigate the effects of canine parvovirus (CPV) non-structural protein-1 (NS1) on the cell apoptosis induced by CPV and preliminarily explore the mechanism of CPV-induced apoptosis. First, the NS1 gene was amplified by PCR from CPV genomic DNA and subcloned into pcDNA3. 1A vector to generate NS1 eukaryotic expression vector pcDNA-NS1. To verify whether pcDNA-NS1 vector can mediate NS1 expression in eukaryotic cells, the human embryo kideny (HEK) 293FT cells were used to transiently express the recombinant NS1. The effects of NS1 on CPV-induced apoptosis were investigated by infecting the F81 host cells with CPV and transfecting the cells with NS1 vector. The apoptosis of the cells was detected by AnnexinV/PI double staining for phosphatidylserine externalization on membrane and by luminescence method for caspase-3/7 activities. The results show that the sequence of NS1 gene amplified was consistent with the GenBank. The NS1 expression vector was shown to be correct and could mediate NS1 expression in eukaryotic cells. The phosphatidylserine on outside of membrane was detected and the caspase-3/7 activities were increased in both CPV-infected cells and NS1-transfected cells. These results indicate that both CPV and NS1 protein can induce the apoptosis of the cells. CPV-induced apoptosis was closely related to its non-structural protein NS1.
Shi H.-Y.,Agricultural University of Hebei |
Zhang Y.-X.,Agricultural University of Hebei
Molecular Biology Reports | Year: 2012
1-Aminocyclopropane-1-carboxylate (ACC) oxidase catalyzes the final reaction of the ethylene biosynthetic pathway, converting ACC into ethylene. Past studies have shown a possible link between ACC oxidase and salicylic acid during fruit ripening in pear, but the relationship has received no more than modest study at the gene expression level. In this study, two cDNA clones encoding putative ACC oxidase, PpACO1 and PpACO2, were isolated from a cDNA library constructed by our own laboratory and produced using mRNA from mesocarp of pear (Pyrus pyrifolia Nakai. cv.Whangkeumbae). One cDNA clone, designated PpACO1 (GenBank accession No. JN807390), comprised an open reading frame of 945 bp encoding a protein of 314 amino acids. The other cDNA, designated PpACO2 (GenBank accession No. JN807392), encodes a protein with 322 amino acids that shares high similarity with the known plant ACOs. Using PCR amplification techniques, two genomic clones corresponding to PpACO1 and PpACO2 were isolated and shown to contain independently three introns with typical GT/AG boundaries defining the splice junctions. The PpACO1 gene product shared 99 % identity with an ACC oxidase from pear (Pyrus 9 bretschneideri Rehd.cv.Yali), and phylogenetic analyses clearly placed the gene product in the ACC oxidase cluster of the pear 2-oxoglutarate-dependent dioxygenase superfamily tree. Quantitative RT-PCR analysis indicated that the two PpACO genes are differentially expressed in pear tissues. PpACO1 and PpACO2 were predominantly expressed in fruit. The transcripts of PpACO1 were accumulated at relatively low levels in early fruit, but strongly high levels in fruit ripening and senescence stages, while the transcripts of PpACO2 were accumulated at higher levels in early fruit and much lower levels with further fruit cell development than the transcripts of PpACO1. In addition, PpACO1 gene was down-regulated in fruit by salicylic acid (SA). Nevertheless, PpACO2 gene was dramatically up-regulated in fruit by SA. These results suggested that the PpACOs may participate in regulation of fruit ripening and in response to SA in pear. © Springer Science+Business Media B.V. 2012.
Zhang K.,Agricultural University of Hebei
Applied Mechanics and Materials | Year: 2014
This paper introduces the working principle of remote access system based on SSL VPN in cloud computing. Meanwhile, it focuses on the operation principle of the SSL protocol and resource management. In addition, it analyses system characteristics such as https login, hardware signature certification and so on. At last, it puts forward some problems and solutions in actual use. © (2014) Trans Tech Publications, Switzerland.