Alfonso D.,Agricultural University of Havana |
Cicatelli A.,University of Salerno |
Guarino F.,University of Salerno |
Rodriguez D.,Agricultural University of Havana |
Castiglione S.,University of Salerno
Plant Biosystems | Year: 2017
Leucocroton havanensis Borhidi is an endemic plant species of Cuba able to hyperaccumulate nickel. In order to sustain the conservation of this species, an efficient protocol for its micropropagation, via axillary bud proliferation, is described. We placed apical segments from aseptic seedlings on basal medium supplemented with indole-3-acetic acid (IAA) and 6-benzylaminopurine (BAP) or thidiazuron (TDZ), individually or in combination. On a medium containing 0.5 mg L−1 IAA and 1.0 mg L−1 BAP explants (65.5%) developed axillary buds. Nevertheless, combinations of 0.5 mg L−1 IAA with 0.1 mg L−1 TDZ was the most effective treatment producing the highest number of buds per explant (30.3); while the control treatment, without growth regulators, produced no buds at all. Transfer of buds to medium supplemented with indole-3-butyric acid, indicated that 0.25 mg L−1 is the amount of hormone required to generate roots on young buds (100%). In order to assess DNA variations in micropropagated plants, an Amplified Fragment Length Polymorphism analysis was performed and no genetic variation was detected. This study demonstrates that a high multiplication rate can be obtained by means of the reported protocol, and that plantlets can be readily hardened (96% survival) in a greenhouse by transplanting them on serpentine soil. © 2017 Società Botanica Italiana
Aguayo M.D.Z.,Technical University of Manabí |
Ruano M.P.,Agricultural University of Havana |
Villafuerte X.R.,Amazonian State University
Revista de Investigaciones Veterinarias del Peru | Year: 2016
A cross-sectional epidemiological study was carried out to determine animal and herd risk factors associated to bovine brucellosis. It was randomly selected 2369 cattle in the Manabí province, Ecuador. In addition, an epidemiological study of ecological type was carried out using 20 positive herds (cases) and 20 negative herds (controls) to the disease. In both studies, an epidemiological survey was conducted to determine the main risk factors associated to the disease. The individual study showed a higher risk of having the disease in animals older than 5 years (p<0.0001) and in females (p<0.05). Herds involved in milk production (p<0.05), those that do not check the aniamls before entering the premises (p<0.05) and those without vaccination (p<0.001) had a higher risk of being affected by the disease. It was also demonstrated that there was a greater probability of having abortions in infected herds than in those unaffected (p< 0.02). It is concluded that there was an increased risk of presenting the disease in animals over 5 years old, in dairy herds and in those that do not check the health status of animals prior to the introduction to the farm and in those without vaccination.
Arencibia-Arrebola D.F.,Instituto Finlay |
Rosario-Fernandez L.A.,Institute Farmacia y Alimentos IFAL |
Suarez-Fernandez Y.E.,Agricultural University of Havana
Revista Internacional de Andrologia | Year: 2011
Objective: This article has aimed to compare Balb/c, NMRI, OF-1 and C57BL/6/cenp mice as a biomodel in the assay of the sperm head basal cavity morphology, keeping in mind the frequency of anomalous forms of the basal head and cyclophosphamide-induced (CF) abnormalities. Materials and methods: Twenty animals/group/mice lines were used, administered for 35 days. A negative control group (not administered), two substance-vehicle controls and a positive control administered with 50. mg/kg FG intraperitoneally for five consecutive days were used. Results and discussion: The best experimental biomodel was with the Balb/c mice, with significant difference with the results obtained in the other lines, keeping in mind the spontaneous and induced values in the sperm concentration and frequency of anomalous epididymal sperms. This study will make it possible to use this mice species more efficiently in genotoxic and preclinical toxicology fertility evaluation of drugs, vaccines and other products. © 2011 Sociedad Española de Andrología.
Dominguez Y.,Mogi Das Cruzes University |
Dominguez Y.,Agricultural University of Havana |
Valdes C.M.P.,University of Habana |
De Miranda V.F.O.,São Paulo State University
Phytotaxa | Year: 2014
The carnivorous plant Pinguicula filifolia occurs in western Cuba and shows a restricted distribution due to habitat specificity associated with poor wet soils. Its populations are distributed mainly in south Pinar del Río (Cuba) and in Isla de la Juventud. Plants from one isolated location in NW Pinar del Río shows morphological differences with respect to the rest of the known populations observed in nature. Subsequent detailed morphological analysis of specimens led to the description of Pinguicula filifolia subsp. alba as a new subspecies. It differs from the typical subspecies in several reproductive traits involving flower, fruit and seed characters and is so far known only from the type locality. © 2014 Magnolia Press.
Machado N.,Agricultural University of Havana |
Balmaseda C.,Agricultural University of Havana |
Montoyo A.,University of Alicante
Iberian Conference on Information Systems and Technologies, CISTI | Year: 2014
People use the similarity to store and retrieve information comparing new situations with similar experiences occurred in the past also for learning and concept formation. While equality comparison between two objects can be calculated by computers quickly and accurately similarity is a complex problem to calculate. Even still difficult to determine similarity plays a fundamental role in many applications such as decision-making systems, data mining and pattern recognition. The same applies to the spatial similarity in the processes of recovery and integration of spatial information. In this paper a methodology based on the semantic processing of geospatial information is proposed. It consists of five stages: conceptualization, synthesis, application processing, retrieval and management. It uses ontology of New Genetic Soil Classification of Cuba and applying the measure of semantic similarity Conceptual Distance (DIS-C) combined with an implementation model TDD (Topology-Direction-Distance) to restore and support the user in the selection of spatial scenes. As a case study is considered the town of San Jose de Las Lajas located in the province of Mayabeque in western Cuba. © 2014 AISTI.
Perez S.,Agricultural University of Havana |
Ahmed A.I.S.,Plant Pathology Unit |
Cabezas D.,Agricultural University of Havana
Biotecnologia Aplicada | Year: 2013
Two different Pb concentrations (5 and 10 mg/kg of PbAc2) were used to study the response of Solanum lycopersicum L. cv. Micro-Tom (MT) plants in two vegetative growth phases. Two genes that have been previously reported to be heavy metal inducible genes (superoxide dismutase, SOD, EC 18.104.22.168; and isofl avone reductase, IFR; EC 22.214.171.124) and another one putatively interesting in this fi eld (transcriptionally controlled tumor protein, TCTP) were selected for an expression study using a real time PCR technique. In the fi rst growth phase (germination to fl owering) TCTP was repressed at 10 mg/kg and in the control, SOD expression was low at both concentrations (5 and 10 mg/kg of PbAc2) and IFR was higher at 10 mg/kg PbAc2. In the second phase (fl owering to fructifi cation) three genes were expressed in both concentrations but in the case of TCTP and SOD the expression was higher at 5 mg/kg of PbAc2. SOD and Glutathione reductase (GR; EC 126.96.36.199) were selected for a biochemical study together with the determination of protein concentration using spectrophotometer. SOD was higher at 10 mg/kg PbAc2 showing signifi cant difference with control, and GR had the same behavior but had signifi cant differences with the other two treatments while total proteins were higher for the control showing signifi cant differences at 10 mg/kg of PbAc2. This research suggests that Pb toxicity leads to the induction of key enzymes of antioxidant defense system in tomato plants. © Biotecnología Aplicada 2013.
Carrasco R.C.,University of Alicante |
Serrano A.,University of Alicante |
Castillo-Buergo R.,Agricultural University of Havana
Information Processing and Management | Year: 2016
Bibliographic collections in traditional libraries often compile records from distributed sources where variable criteria have been applied to the normalization of the data. Furthermore, the source records often follow classical standards, such as MARC21, where a strict normalization of author names is not enforced. The identification of equivalent records in large catalogues is therefore required, for example, when migrating the data to new repositories which apply modern specifications for cataloguing, such as the FRBR and RDA standards. An open-source tool has been implemented to assist authority control in bibliographic catalogues when external features (such as the citations found in scientific articles) are not available for the disambiguation of creator names. This tool is based on similarity measures between the variants of author names combined with a parser which interprets the dates and periods associated with the creator. An efficient data structure (the unigram frequency vector trie) has been used to accelerate the identification of variants. The algorithms employed and the attribute grammar are described in detail and their implementation is distributed as an open-source resource to allow for an easier uptake. © 2016 Elsevier Ltd.
Land cover mapping based on the spectral-temporary Landsat 7-ETM+ images classification and in a land cover classes legend in a Cuban western territory [Cartografía de la cobertura de la tierra basada en la clasificación espectro-temporal de imágenes Landsat 7-ETM+ y en una leyenda de clases de ocupación del suelo en un territorio de Cuba occidental]
Ponvert-Delisles Batista R.D.,Agricultural University of Havana |
Reyes Alonso I.,UCT
Revista de Teledeteccion | Year: 2013
The environmental studies generally need to have information about the territory of study is ocupied. This information on the classes of land cover is represented in different types of thematic maps. The present work has as objective to generate the cartography of soil occupation in a territory of western Cuba, using as basic geospatial data multispectral images of the Landsat 7-ETM+ (SLC-off) satellite from two dates and as main auxiliary data the Legend of Classes of Soil Occupation, wichs offer a categorization of the classes bay lebels and themes. The methodology used is based on carrying out supervised classifications supported in the spectral-temporary visual analysis to identify certain problematic classes that have varied its behavior between the two dates and finally to obtain a thematic map that reflects these changes with the major efficiency.
Quindemil Torrijo E.M.,Agricultural University of Havana
ACIMED | Year: 2010
Some considerations are exposed for the introduction of the information literacy in the specialty of Library and Information Science, a model continuity of studies. A technique of the documental analysis was used in the revision of documents that shows the contemporary pedagogic models and the main contributions that serve as a base before the designing of educational proposals. Also, the revision of the study plan allowed considering the employment of methods for the formation for competitions: problem based learning and project oriented learning, taking as an alternative in the design of an educational proposal of information literacy for the development of the informational competence in this professional. ©ECIMED.
Baez Y.A.,Agricultural University of Havana |
Jimenez R.C.C.,University of Alicante
Proceedings - 12th International Conference on Computational Science and Its Applications, ICCSA 2012 | Year: 2012
Path indexes based on suffix trees have shown to be highly efficient structures when dealing with digital collection that consists of structured documents, since they provide a fast response to queries including structural requirements. Nevertheless, when the collection consists of highly heterogeneous documents, suffix trees may be too memory demanding. In such cases, the use of a suffix array as the underlying data storage permits a considerable reduction in space requirements, partially because suffix arrays are a remarkably light data structure and partially because they do not store redundant information regarding the textual content. We describe how a suffix array can be used as the data structure which stores the structural index in a retrieval system and provides a virtual index of all sub paths in the digital collection. We also show how an auxiliary ternary search tree can accelerate the resolution of structural queries with only a marginal increase in memory usage. © 2012 IEEE.