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Kuo C.-M.,National Chiao Tung University | Chen T.-Y.,National Chiao Tung University | Lin T.-H.,National Chiao Tung University | Kao C.-Y.,National Chiao Tung University | And 4 more authors.
Bioresource Technology

The development of a culture system for Chlorella sp. GD to efficiently produce biomass and oil for biodiesel production was investigated. Chlorella sp. GD was cultivated with 0%, 25%, 50%, 75% and 100% piggery wastewater (diluted by medium) at 300μmolm-2s-1, a 2% CO2 aeration rate of 0.2vvm and 26±1°C; after a 10-day culture in batch cultures, the maximum specific growth rate and biomass productivity of the microalga obtained in 100% piggery wastewater were 0.839d-1 and 0.681gL-1d-1, respectively. The highest lipid content and lipid productivity were 29.3% and 0.155gL-1d-1 at 25% wastewater, respectively. In semi-continuous cultures, the biomass and lipid productivities with 25-75% wastewater ratios were greater than 0.852 and 0.128gL-1d-1, respectively. These results show that Chlorella sp. GD grows efficiently in piggery wastewater, and that a stable growth performance was achieved for long-term microalgal cultivation in a semi-continuous culture. © 2015 . Source

Wu C.-C.,National Yang Ming University | Weng W.-L.,National Yang Ming University | Lai W.-L.,Chung Shan Medical University | Tsai H.-P.,National Yang Ming University | And 3 more authors.
Evidence-based Complementary and Alternative Medicine

Recent studies have demonstrated beneficial effects of specific probiotics on alleviating obesity-related disorders. Here we aimed to identify probiotics with potential antiobesity activity among 88 lactic acid bacterial strains via in vitro screening assays, and a Lactobacillus plantarum strain K21 was found to harbor abilities required for hydrolyzing bile salt, reducing cholesterol, and inhibiting the accumulation of lipid in 3T3-L1 preadipocytes. Furthermore, effects of K21 on diet-induced obese (DIO) mice were examined. Male C57Bl/6J mice received a normal diet, high-fat diet (HFD), or HFD with K21 administration (109 CFU in 0.2 mL PBS/day) for eight weeks. Supplementation of K21, but not placebo, appeared to alleviate body weight gain and epididymal fat mass accumulation, reduce plasma leptin levels, decrease cholesterol and triglyceride levels, and mitigate liver damage in DIO mice. Moreover, the hepatic expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) related to adipogenesis was significantly downregulated in DIO mice by K21 intervention. We also found that K21 supplementation strengthens intestinal permeability and modulates the amount of Lactobacillus spp., Bifidobacterium spp., and Clostridium perfringens in the cecal contents of DIO mice. In conclusion, our results suggest that dietary intake of K21 protects against the onset of HFD-induced obesity through multiple mechanisms of action. © 2015 Chien-Chen Wu et al. Source

Chiu S.-Y.,National Chiao Tung University | Chiu S.-Y.,Industrial Technology Research Institute of Taiwan | Kao C.-Y.,National Chiao Tung University | Kao C.-Y.,Agricultural Technology Research Institute | And 4 more authors.
Bioresource Technology

Using wastewater for microalgal cultures is beneficial for minimizing the use of freshwater, reducing the cost of nutrient addition, removing nitrogen and phosphorus from wastewater and producing microalgal biomass as bioresources for biofuel or high-value by-products. There are three main sources of wastewater, municipal (domestic), agricultural and industrial wastewater, which contain a variety of ingredients. Some components in the wastewater, such as nitrogen and phosphorus, are useful ingredients for microalgal cultures. In this review, the effects on the biomass and lipid production of microalgal Chlorella cultures using different kinds of wastewater were summarized. The use of the nutrients resource in wastewater for microalgal cultures was also reviewed. The effect of ammonium in wastewater on microalgal Chlorella growth was intensively discussed. In the end, limitations of wastewater-based of microalgal culture were commented in this review article. © 2014 Elsevier Ltd. Source

Cheng C.Y.,National Taiwan University | Lu C.F.,Agricultural Technology Research Institute | Chen Y.H.,National Taiwan University
Journal of International Trade and Economic Development

This study has used the time series data of Australia, China, Denmark, India, Japan, the Netherlands, Norway, the Philippines, South Korea, Spain, and the United States from the World Bank to estimate the agricultural economic shock of Taiwan's accession to the World Trade Organization (WTO). We have adopted the recently developed panel data approach for policy evaluation to construct the annual agricultural growth path of Taiwan, which is mainly based on the cross-sectional correlations between the domestic and international agricultural trade markets in the absence of Taiwan's entry into the WTO. Our results have not only revealed the importance of ex post counterfactual analysis, but also provided empirical evidence that the agricultural economic shock is not as severe as predicted by many ex ante studies. Based on these results, we have concluded that the outcome may have arisen from the slow progress of achieving agricultural trade liberalization under the WTO and the Taiwanese Government's effective adoption of phase-in periods and relative adjustment policies. © 2016 Taylor & Francis. Source

Agricultural Technology Research Institute | Date: 2014-06-30

The invention provides a recombinant polypeptide X-Y for enhancing cell transduction efficiency of a target agent, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Also provided is a method for enhancing cell transduction efficiency of a target agent, comprising conjugating/attaching said target agent with a recombinant polypeptide X-Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Further provided is a pharmaceutical composition comprising a therapeutic agent, wherein said therapeutic agent is modified by conjugating/attaching with a recombinant polypeptide X-Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain.

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