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Xiao G.,Zhejiang University of Science and Technology | Xiao H.,Zhejiang University | Xiao H.,Agricultural Products Processing Technology Key Laboratory of Zhejiang Province | Xiao H.,Hangzhou Institute of Calibration and Testing for Quality and Technical Supervision | And 2 more authors.
Dairy Science and Technology | Year: 2014

The determination of nucleotide profile in human milk is an important issue for making nucleotide-fortified dairy products. Here, a method for the simultaneous quantitation of five monophosphate nucleotides (cytidine 5′-monophosphate (CMP), uridine 5′-monophosphate (UMP), adenosine 5′-monophosphate (AMP), inosine 5′-monophosphate (IMP), guanosine 5′-monophosphate (GMP)) in milk using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The nucleotides were separated using a C18 column and gradient elution with water containing 0.1% (v/v) formic acid and acetonitrile. The method was validated with spike recoveries of 90.3-102.7% and 81.2-96.2%, and repeatability relative standard deviations of 2.5-4.3% and 2.6-4.6% for fresh bovine milk and milk powder, respectively. The method was applied for the assay of Chinese human milk samples collected from 59 nursing mothers with 2-4 months of lactation in Zhejiang, China. The mean (±SD) contents of CMP, UMP, AMP, IMP, and GMP are 3.1∈±∈1.8, 2.2∈±∈2.2, 0.23∈±∈0.18, 0.018∈±∈0.023, and 0.24∈±∈0.19 mg.kg-1, respectively. The results of this work may serve as a guideline for the humanizing bovine milk-based infant formulas by supplementing the five monophosphate nucleotides for Chinese infants. © 2014 INRA and Springer-Verlag France.


Jia S.,Zhejiang University | Hu Y.,Zhejiang University | Zhang W.,Zhejiang University | Zhao X.,Zhejiang University | And 7 more authors.
Food and Function | Year: 2015

The present study is to investigate the possible hypoglycemic and hypolipidemic effects of neohesperidin (NHP) derived from Citrus aurantium L. in vivo. KK-Ay mice were used as the diabetic experimental model, whereas C57BL/6 mice were used as normal control for a 6-week study. Treatment of NHP significantly decreased fasting glucose, serum glucose, and glycosylated serum protein (GSP) in KK-Ay mice. It significantly elevated oral glucose tolerance and insulin sensitivity and decreased insulin resistance in the diabetic mice. In addition, NHP significantly decreased serum triglycerides (TG), total cholesterol (TCH), leptin level, and liver index in the KK-Ay mice. NHP also inhibited lipid accumulation in the liver and decreased the size of epididymal adipocyte in the KK-Ay mice. Gene expression of stearoyl-CoA desaturase 1 (SCD-1) and fatty acid synthase (FAS) were significantly inhibited, whereas the expression of acyl-CoA oxidase (ACOX) was significantly induced by NHP treatment in the liver of KK-Ay mice. In addition, elevated level of phosphorylation of hepatic AMPK was observed in NHP-treated mice. Therefore, the activation of the AMPK pathway and regulation of its target genes, including SCD-1, FAS, and ACOX, may play important roles in the hypoglycemic and hypolipidemic effects of NHP in vivo, and NHP may have great potential in the prevention of diabetes and its complications. This journal is © The Royal Society of Chemistry 2015.

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