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Chen Y.-G.,Huazhong Agricultural University | Chen Y.-G.,Spice and Beverage Research Institute | Gu F.-L.,Spice and Beverage Research Institute | Gu F.-L.,Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops | And 8 more authors.
Current Microbiology | Year: 2015

A Gram-positive bacterium, designated strain XY18T, was isolated from a cured vanilla bean in Hainan province, China. Cells were rod-shaped, endospore producing, and peritrichous flagella. Strain XY18T grew at salinities of 0–8 % (w/v) NaCl (optimally 1–4 %), pH 4.0–8.0 (optimally 5.0–7.0 %) and temperature range 20–45 °C (optimally 28–35 °C). The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15:0, iso-C15:0, anteiso-C17:0, and iso-C17:0. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain XY18T was a member of the genus Bacillus, and closely related to B. amyloliquefaciens NBRC 15535T and B. siamensis PD-A10T, with 99.1 and 99.2 % sequence similarity, respectively. However, the DNA–DNA hybridization value between strain XY18T and B. amyloliquefaciens NBRC 15535T was 35.7 %. The genomic DNA G+C content of strain XY18T was 46.4 mol%, significantly differed from B. siamensis PD-A10T (41.4 %), which was higher than the range of 4 % indicative of species. On the basis of polyphasic taxonomic study, including phenotypic features, chemotaxonomy, and phylogenetic analyses, strain XY18T represents a novel species within the genus Bacillus, for which the name Bacillusvanillea sp. nov. is proposed. The type strain is XY18T (=CGMCC 8629 = NCCB 100507). © 2014, Springer Science+Business Media New York.


Chen Y.,Spice and Beverage Research Institute | Chen Y.,Huazhong Agricultural University | Chen Y.,Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops | Gu F.,Spice and Beverage Research Institute | And 8 more authors.
Applied and Environmental Microbiology | Year: 2015

Vanilla beans were analyzed using biochemical methods, which revealed that glucovanillin disperses from the inner part to the outer part of the vanilla bean during the curing process and is simultaneously hydrolyzed by β-D-glucosidase. Enzymatic hydrolysis was found to occur on the surface of the vanilla beans. Transcripts of the β-D-glucosidase gene (bgl) of colonizing microorganisms were detected. The results directly indicate that colonizing microorganisms are involved in glucovanillin hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that the colonizing microorganisms mainly belonged to the Bacillus genus. bgl was detected in all the isolates and presented clustering similar to that of the isolate taxonomy. Furthermore, inoculation of green fluorescent protein-tagged isolates showed that the Bacillus isolates can colonize vanilla beans. Glucovanillin was metabolized as the sole source of carbon in a culture of the isolates within 24 h. These isolates presented unique glucovanillin degradation capabilities. Vanillin was the major volatile compound in the culture. Other compounds, such as α-cubebene, β-pinene, and guaiacol, were detected in some isolate cultures. Colonizing Bacillus isolates were found to hydrolyze glucovanillin in culture, indirectly demonstrating the involvement of colonizing Bacillus isolates in glucovanillin hydrolysis during the vanilla curing process. Based on these results, we conclude that colonizing Bacillus isolates produce β-D-glucosidase, which mediates glucovanillin hydrolysis and influences flavor formation. © 2015, American Society for Microbiology.


Li C.,Deakin University | Li Y.,Deakin University | Li Y.,Agricultural Product Processing Research Institute | She X.,Deakin University | And 5 more authors.
Composites Science and Technology | Year: 2015

We report the synergistic reinforcement and deformation of polyvinyl alcohol (PVA)/graphene/montmorillonite clay (MMT) composites with the tensile properties being improved greatly. Particularly, the tensile strength and modulus of PVA composite with 0.9 wt% graphene and 0.3 wt% of MMT were improved by more than 58% and 43% when compared to the neat PVA, respectively, and were at least 10% higher than the enhanced sum of dual PVA composites with 0.9 wt% graphene and 0.3 wt% MMT. This reinforcement was resulted from the good dispersion and effective interfacial interactions as confirmed from morphology investigation, increased glass transition temperature and the shift of O-H stretching. When there were no fillers i.e. in situ reduced graphene (IRG) or MMT or their loading was low, high alignment of PVA could be observed, with increased crystallinity, melting point, lamellae thickness but narrowed crystallite size distribution. The synergistic reinforcement of PVA achieved from combined incorporation of IRG and MMT will pave the way for the development of stronger PVA composites in various applications. © 2015.


Zhou M.,Agricultural Product Processing Research Institute | Li P.,Agricultural Product Processing Research Institute | Wang G.,Agricultural Product Processing Research Institute | Yang Z.,Agricultural Product Processing Research Institute | And 2 more authors.
Applied Mechanics and Materials | Year: 2012

Functionalized chitosan (CS) were widely used as drug delivery system in the chemotherapy of various disease. In this work, folate (FA) was conjugated into chitosan molecular as targeting ligand based on Schiff reaction between -NH 2 group of CS and -COOH group of FA. And nanoparticles were made by emulsion method with vanillin novel cross-linking agent. The FA modified CS and its nanoparticles were characterized by Fourier transform spectroscopy (FT-IR), scanning electron microscope (SEM) and Zeta potential. SEM results confirmed the nanoparticles made from FA-CS conjugate were spherical in shape and were about 100 nm in size. Zeta potential analysis revealed that the nanoparticles were negatively charged with charge density of -7.73mV. © (2012) Trans Tech Publications.


Kong L.X.,Deakin University | Wang Q.,State Engineering and Technology Research Center for Key Tropical Crops | Luo Y.,Agricultural Product Processing Research Institute | Feng C.,Agricultural Product Processing Research Institute | And 5 more authors.
Journal of Nanomaterials | Year: 2012

A highly performing natural rubber/silica (NR/SiO2) nanocomposite with a SiO2 loading of 2wt was prepared by combining similar dissolve mutually theory with latex compounding techniques. Before polymerization, double bonds were introduced onto the surface of the SiO2 particles with the silane-coupling agent. The core-shell structure silica-poly(methyl methacrylate), SiO2-PMMA, nanoparticles were formed by grafting polymerization of MMA on the surface of the modified SiO2 particles via in situ emulsion, and then NR/SiO2 nanocomposite was prepared by blending SiO2-PMMA and PMMA-modified NR (NR-PMMA). The Fourier transform infrared spectroscopy results show that PMMA has been successfully introduced onto the surface of SiO2, which can be well dispersed in NR matrix and present good interfacial adhesion with NR phase. Compared with those of pure NR, the thermal resistance and tensile properties of NR/SiO2 nanocomposite are significantly improved. © 2012 Qinghuang Wang et al.


Zhang F.Q.,Agricultural Product Processing Research Institute | Wang Y.Z.,Agricultural Product Processing Research Institute | Chen M.,Agricultural Product Processing Research Institute | Huang M.F.,Agricultural Product Processing Research Institute
Applied Mechanics and Materials | Year: 2014

In this article, the molecular weight of wet Natural Rubber (NR) with different initial moisture contents, 3.80%, 10.05%, 19.76%, respectively, dried by microwave at frequency of 2450MHz was investigated using GPC. It can be seen from the results that an obvious trend of Mn and Mw changes was presented. With the increment of initial moisture contents, Mn and Mw decreased gradually. The molecular weight distribution (d) increased with the initial moisture contents increment, but quite closer to each other. © (2014) Trans Tech Publications, Switzerland.


Zhang F.Q.,Agricultural Product Processing Research Institute | Wang Y.Z.,Agricultural Product Processing Research Institute | Chen M.,Agricultural Product Processing Research Institute | Huang M.F.,Agricultural Product Processing Research Institute | Zeng R.Z.,Rubber Research Institute of China
Advanced Materials Research | Year: 2014

In this work, the rheological behavior of uncured natural rubber with different initial moisture contents dried by microwave was studied using RPA under frequency sweep and strain sweep modes. It can be seen from the results that the variation trend of viscous torque S' versus sweep frequency kept with the trend of elastic torque S' versus sweep frequency. Moreover, the viscous torque S' values were lower than those for elastic torque S', a good linear relation curve was obtained by plotting elastic torque S' versus sweep frequency. The curve of storage shear modulus G' versus sweep strain presented a linear relationship, showed a linear viscoelastic behavior of uncured natural rubber with different initial moisture contents. This may be explained by the degradation of molecular chains of natural rubber and the van der waals forces between them. © (2014) Trans Tech Publications, Switzerland.


Wang Y.-Z.,Agricultural Product Processing Research Institute | Zhang F.-Q.,Agricultural Product Processing Research Institute | Chen M.,Agricultural Product Processing Research Institute | Lv M.-Z.,Agricultural Product Processing Research Institute | Huang H.-H.,Agricultural Product Processing Research Institute
Advanced Materials Research | Year: 2011

In this work, the cross-linking density and frequency sweep, temperature sweep of NR dried by microwave were tested by using nuclear magnetic resonance spectrometer (NMR) and rubber process analyzer (RPA), respectively. And the test results were compared with those of NR dried by hot-air. The results showed that the cross-linking density and A(Mc) for NR dried by microwave were bigger than those for NR dried by hot-air, but the A(T2) of latter was bigger; the frequency sweep results showed that the elastic modulus of NR dried by hot-air increased slower with the increase of frequency than those of NR dried by microwave , and the tand of NR dried by hot-air decreased with the increase of frequency slower than those of NR dried by microwave in low frequency phase, respectively; the temperature sweep results showed that the elastic modulus of NR dried by hot air were smaller compared with those of NR dried by microwave in the entire scanning process, but, the tand value of former was bigger. © (2011) Trans Tech Publications.


PubMed | Agricultural Product Processing Research Institute
Type: Journal Article | Journal: Drug delivery | Year: 2014

The present study aims to prepare and evaluate carbon nanotubes (CNTs)-based colon-specific microspheres using irinotecan as a model of drug. The synthesis of CNTs-based microspheres including attachment of folate-chitosan conjugate and irinotecan to CNTs via non-covalent interaction, followed by microencapsulation with Eudragit S-100 by an oil-in-oil solvent evaporation technique. The obtained samples were characterized in case of surface morphology, drug loading efficiency and particle size. In vitro drug release behavior was studied in different pH medium and the obtained data were subjected to kinetic equations. It was found that the Eudragit-coated microparticles were spherical with smooth surface, and the particle size varied with the core/coat ratio. In vitro drug release shows that the irinotecan released in a slow and sustained fashion from the CNTs-based carriers without coating with Eudragit. No drug release was observed from Eudragit-coated microspheres when the medium pH below 7, while when the pH reached 7.4, the coating layer of Eudragit began to dissolve and a controlled release of irinotecan was observed. The cell viability test indicates that the drug free FA-CS decorated CNTs had no influence on the cell proliferation rates of HT-29 cells, while the irinotecan-loaded CNTs drug system proved to be the most cytotoxic.


Zhang F.Q.,Agricultural Product Processing Research Institute | Wang Y.Z.,Agricultural Product Processing Research Institute | Chen M.,Agricultural Product Processing Research Institute | Huang M.F.,Agricultural Product Processing Research Institute | Zeng R.Z.,Rubber Research Institute of China
Applied Mechanics and Materials | Year: 2014

In this work, the crosslink density of uncured natural rubber with different initial moisture contents, 3.80%, 10.05%, 19.76%, 23.68%, respectively, dried by microwave at frequency of 2450MHz was studied using nuclear magnetic resonance spectrometer. Seen from the results, the effect of initial moisture content of wet natural rubber on crosslink density and processability were obvious. With the increment of initial moisture content of wet natural rubber, the crosslink density of natural rubber after microwave dried with the frequency of 2450MHz decreased gradually. The crosslink density and A(Mc) of natural with the lower initial moisture content was bigger compared with natural rubber with the higher initial moisture content. © (2014) Trans Tech Publications, Switzerland.

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