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Çobanlar, Turkey

Madi N.,American University of Beirut | Dany M.,Medical University of South Carolina | Abdoun S.,Agricultural Engineer | Usta J.,American University of Beirut
Journal of the American College of Nutrition | Year: 2016

Introduction: Moringa oleifera (MO) is an important dietary component for many populations in West Africa and the Indian subcontinent. In addition to its highly nutritious value, almost all parts of this plant have been widely used in folk medicine in curing infectious, cardiovascular, gastrointestinal, hepatic, and other diseases. Evidence-based research supported its versatile medicinal properties; however, more rigorous research is required to establish it in cancer therapy. As such, in this study we aim to investigate the in vitro anticancerous effect of Moringa oleifera's aqueous leaf extract. Methods: Moringa extract was prepared by soaking pulverized leaves in hot water mimicking the people's mode of the leaf drink preparation. Several assays were used to study the effect of different percentage concentrations of the extract on viability of A549 cells; levels of adenosine triphosphate (ATP), reactive oxygen species (ROS), and glutathione (GSH) generated; as well as percentage of lactate dehydrogenase (LDH) released at different time points. In addition to mitochondrial membrane potential, apoptotic events were assessed using western blotting for apoptotic markers and immunoflourescent flourescent labeled inhibitor of caspases (FLICA) assay. Results: MO extract treatment resulted in a significant decrease in mitochondrial membrane potential (1 hour) and ATP levels (3 hours), followed by an increase in (6 hours) ROS, caspase activation, proapoptotic proteins expression (p53, SMAC/Diablo, AIF), and PARP-1 cleavage. This eventually resulted in decreased GSH levels and a decrease in viability. The cytotoxic effect was prevented upon pretreatment with antioxidant N-acetyl-cysteine. MO decreased as well the viability of HepG2, CaCo2, Jurkat, and HEK293 cells. Conclusion: Our findings identify a plant extract with an anticancerous effect on cancer cell lines. MO extract exerts its cytotoxic effect in A549 cancer cells by affecting mitochondrial viability and inducing apoptosis in an ROS-dependent manner. 2016 © American College of Nutrition Source


Turan M.A.,Uludag University | Elkarim A.H.A.,University of Khartoum | Taban N.,Agricultural Engineer | Taban S.,Kastamonu University
African Journal of Agricultural Research | Year: 2010

In this study, effect of applied NaCl on shoot and root growth and distribution and accumulation of Na, Cl, N, P, K, Ca, Fe, Zn and Mn in shoot and root of the maize plant (Zea mays L. cv: RX 947) was investigated. The experiment was arranged in a completely randomized design (CRD) under the greenhouse condition. Sodium chloride was applied at the rates of 0, 25, 50, 75 and 100 mM NaCl. Shoot and root growth of the maize plants was inhibited by salinity and NaCl significantly decreased shoot and root dry mass of maize plants. Sodium chloride caused to increase Na, Cl, P, Zn and Mn concentrations in the shoot and root. Applied NaCl decreased N, Ca and Fe concentrations in the shoot, increased N, Ca and Fe concentrations in the root. Sodium, Cl, N, P, Ca, Fe, Zn and Mn accumulated in the root in according to applied NaCl. © 2010 Academic Journals. Source


Turan M.A.,Uludag University | Taban N.,Agricultural Engineer | Turkmen N.,Ankara University | Taban S.,Kastamonu University
Asian Journal of Chemistry | Year: 2010

The aim of this study is to evaluate the selenium concentration of garlic bulbs, which are widely grown in different parts of Turkey. For this purpose, 88 samples of garlic bulbs were collected from eight intensive garlic growing areas of Turkey, namely, Kastamonu (23), Balikesir (18), Kirklareli (11), Mugla (9), Kahramanmaras (8), Karaman (6), Hatay (8) and Antalya (5). The selenium concentrations of the garlic bulbs were determined using ICP-OES techniques. The selenium concentrations in the dry and fresh weight of garlic bulbs were determined as being in the ranges 1.85-9.33 mg/kg and 0.46-2.33 mg/kg, respectively. The overall average of the 88 samples was found to be 3.87 mg/kg in dry weight and 0.97 mg/kg in fresh weight. As a result, the garlic bulbs from Kastamonu showed the highest selenium concentration, which indicates that the recommended dietary allowance of selenium, 70 micrograms, could be supplied by these garlic bulbs. Source


Farah A.B.,Agricultural Engineer | Gomez-Ramos A.,University of Valladolid
Sustainability (Switzerland) | Year: 2014

The "Europe 2020 Strategy" launched by European Institutions is a commitment to increase growth based on the coexistence of both competitiveness and sustainable development. This paper analyzes the competitiveness of production systems in the cereal steppes of Castile, Spain. An indicator based on each production system's profitability threshold was built. The diagnostic analysis methodology allowed the identification of 20 production system models related to agrarian, livestock and mixed farming systems. The results show very different levels of competitiveness which are not necessarily related to the farms' sizes or capitalization levels but mostly to production costs and the farmers' ages. The response of these models to future input and output price scenarios shows that mixed farms are less dependent on external production factors. © 2014 by the authors. Source


Kumar A.,COAE and T | Hooda V.S.,COAE and T | Mukesh S.,Agricultural Engineer
Indian Journal of Agricultural Sciences | Year: 2015

A two-year field study was conducted during 2011 and 2012 at Karnal (Haryana), to evaluate tillage and crop establishment (TCE) options with an objective to improve yield, water productivity and profitability in rice (Oryza sativa L.). The TCE methods had a significant effect on rice yields. Grain yield of mechanically transplanted rice (MTR) in puddled soil was significantly higher (10-22%) than all the other treatments in both the years of study. Conventional till direct seeded rice (CT-DSR) and zero-till direct seeded rice (ZT-DSR) consumed 12-17% less water as compared to puddled transplanted (manual or mechanical) rice during 2011, whereas during 2012, it consumed 5-9% more water than puddled transplanted rice. DSR (CT or ZT) and mechanical transplanting (under both puddled and unpuddled conditions) resulted in a labour saving of 7-8% and 6-12% as compared to manual puddled transplanting, respectively. The benefit: cost ratio was highest in MTR in puddled soil followed by ZT-DSR and was minimum in mechanically transplanted rice in unpuddled soil. Energy output: input ratio was highest in MTR (4.1) in puddled soil followed by manual transplanting (3.7) and was minimum in MTR in unpuddled soil (3.2). Source

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